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Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi

Bacterial diseases caused by Vibrio spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant Vibrio strains, phage therapy is being explored as a potential alternative to antibio...

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Autores principales: Li, Yingying, Yun, Huayi, Chen, Ruo, Jiao, Nianzhi, Zheng, Qiang, Yang, Yunlan, Zhang, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671443/
https://www.ncbi.nlm.nih.gov/pubmed/38003392
http://dx.doi.org/10.3390/ijms242216202
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author Li, Yingying
Yun, Huayi
Chen, Ruo
Jiao, Nianzhi
Zheng, Qiang
Yang, Yunlan
Zhang, Rui
author_facet Li, Yingying
Yun, Huayi
Chen, Ruo
Jiao, Nianzhi
Zheng, Qiang
Yang, Yunlan
Zhang, Rui
author_sort Li, Yingying
collection PubMed
description Bacterial diseases caused by Vibrio spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant Vibrio strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against Vibrio harveyi BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems.
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spelling pubmed-106714432023-11-11 Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi Li, Yingying Yun, Huayi Chen, Ruo Jiao, Nianzhi Zheng, Qiang Yang, Yunlan Zhang, Rui Int J Mol Sci Article Bacterial diseases caused by Vibrio spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant Vibrio strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against Vibrio harveyi BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems. MDPI 2023-11-11 /pmc/articles/PMC10671443/ /pubmed/38003392 http://dx.doi.org/10.3390/ijms242216202 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yingying
Yun, Huayi
Chen, Ruo
Jiao, Nianzhi
Zheng, Qiang
Yang, Yunlan
Zhang, Rui
Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title_full Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title_fullStr Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title_full_unstemmed Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title_short Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi
title_sort characterization of a vibriophage infecting pathogenic vibrio harveyi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671443/
https://www.ncbi.nlm.nih.gov/pubmed/38003392
http://dx.doi.org/10.3390/ijms242216202
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