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Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta

While exercise (EX) during pregnancy is beneficial for both mother and child, little is known about the mechanisms by which maternal exercise mediates changes in utero. Six-week-old female C57BL/6 mice were divided into two groups: with (exercise, EX; N = 7) or without (sedentary, SED; N = 8) access...

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Autores principales: Ruebel, Meghan L., Borengasser, Sarah J., Zhong, Ying, Kang, Ping, Faske, Jennifer, Shankar, Kartik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671464/
https://www.ncbi.nlm.nih.gov/pubmed/38003633
http://dx.doi.org/10.3390/ijms242216441
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author Ruebel, Meghan L.
Borengasser, Sarah J.
Zhong, Ying
Kang, Ping
Faske, Jennifer
Shankar, Kartik
author_facet Ruebel, Meghan L.
Borengasser, Sarah J.
Zhong, Ying
Kang, Ping
Faske, Jennifer
Shankar, Kartik
author_sort Ruebel, Meghan L.
collection PubMed
description While exercise (EX) during pregnancy is beneficial for both mother and child, little is known about the mechanisms by which maternal exercise mediates changes in utero. Six-week-old female C57BL/6 mice were divided into two groups: with (exercise, EX; N = 7) or without (sedentary, SED; N = 8) access to voluntary running wheels. EX was provided via 24 h access to wheels for 10 weeks prior to conception until late pregnancy (18.5 days post coitum). Sex-stratified placentas and fetal livers were collected. Microarray analysis of SED and EX placentas revealed that EX affected gene transcript expression of 283 and 661 transcripts in male and female placentas, respectively (±1.4-fold, p < 0.05). Gene Set Enrichment and Ingenuity Pathway Analyses of male placentas showed that EX led to inhibition of signaling pathways, biological functions, and down-regulation of transcripts related to lipid and steroid metabolism, while EX in female placentas led to activation of pathways, biological functions, and gene expression related to muscle growth, brain, vascular development, and growth factors. Overall, our results suggest that the effects of maternal EX on the placenta and presumably on the offspring are sexually dimorphic.
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spelling pubmed-106714642023-11-17 Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta Ruebel, Meghan L. Borengasser, Sarah J. Zhong, Ying Kang, Ping Faske, Jennifer Shankar, Kartik Int J Mol Sci Article While exercise (EX) during pregnancy is beneficial for both mother and child, little is known about the mechanisms by which maternal exercise mediates changes in utero. Six-week-old female C57BL/6 mice were divided into two groups: with (exercise, EX; N = 7) or without (sedentary, SED; N = 8) access to voluntary running wheels. EX was provided via 24 h access to wheels for 10 weeks prior to conception until late pregnancy (18.5 days post coitum). Sex-stratified placentas and fetal livers were collected. Microarray analysis of SED and EX placentas revealed that EX affected gene transcript expression of 283 and 661 transcripts in male and female placentas, respectively (±1.4-fold, p < 0.05). Gene Set Enrichment and Ingenuity Pathway Analyses of male placentas showed that EX led to inhibition of signaling pathways, biological functions, and down-regulation of transcripts related to lipid and steroid metabolism, while EX in female placentas led to activation of pathways, biological functions, and gene expression related to muscle growth, brain, vascular development, and growth factors. Overall, our results suggest that the effects of maternal EX on the placenta and presumably on the offspring are sexually dimorphic. MDPI 2023-11-17 /pmc/articles/PMC10671464/ /pubmed/38003633 http://dx.doi.org/10.3390/ijms242216441 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruebel, Meghan L.
Borengasser, Sarah J.
Zhong, Ying
Kang, Ping
Faske, Jennifer
Shankar, Kartik
Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title_full Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title_fullStr Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title_full_unstemmed Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title_short Maternal Exercise Prior to and during Gestation Induces Sex-Specific Alterations in the Mouse Placenta
title_sort maternal exercise prior to and during gestation induces sex-specific alterations in the mouse placenta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671464/
https://www.ncbi.nlm.nih.gov/pubmed/38003633
http://dx.doi.org/10.3390/ijms242216441
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