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Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro

Members of the family Coronaviridae cause diseases in mammals, birds, and wildlife (bats), some of which may be transmissible to humans or specific to humans. In the human population, they can cause a wide range of diseases, mainly affecting the respiratory and digestive systems. In the scientific d...

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Autores principales: Hinkov, Anton, Tsvetkov, Venelin, Shkondrov, Aleksandar, Krasteva, Ilina, Shishkov, Stoyan, Shishkova, Kalina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671514/
https://www.ncbi.nlm.nih.gov/pubmed/38003714
http://dx.doi.org/10.3390/ijms242216525
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author Hinkov, Anton
Tsvetkov, Venelin
Shkondrov, Aleksandar
Krasteva, Ilina
Shishkov, Stoyan
Shishkova, Kalina
author_facet Hinkov, Anton
Tsvetkov, Venelin
Shkondrov, Aleksandar
Krasteva, Ilina
Shishkov, Stoyan
Shishkova, Kalina
author_sort Hinkov, Anton
collection PubMed
description Members of the family Coronaviridae cause diseases in mammals, birds, and wildlife (bats), some of which may be transmissible to humans or specific to humans. In the human population, they can cause a wide range of diseases, mainly affecting the respiratory and digestive systems. In the scientific databases, there are huge numbers of research articles about the antiviral, antifungal, antibacterial, antiviral, and anthelmintic activities of medicinal herbs and crops with different ethnobotanical backgrounds. The subject of our research is the antiviral effect of isolated saponins, a purified saponin mixture, and a methanol extract of Astragalus glycyphyllos L. In the studies conducted for the cytotoxic effect of the substances, CC(50) (cytotoxic concentration 50) and MTC (maximum tolerable concentration) were determined by the colorimetric method (MTT assay). The virus was cultured in the MDBK cell line. As a result of the experiments carried out on the influence of substances on viral replication (using MTT-based colorimetric assay for detection of human Coronavirus replication inhibition), it was found that the extract and the purified saponin mixture inhibited 100% viral replication. The calculated selective indices are about 13 and 18, respectively. The obtained results make them promising for a preparation with anti-Coronavirus action.
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spelling pubmed-106715142023-11-20 Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro Hinkov, Anton Tsvetkov, Venelin Shkondrov, Aleksandar Krasteva, Ilina Shishkov, Stoyan Shishkova, Kalina Int J Mol Sci Article Members of the family Coronaviridae cause diseases in mammals, birds, and wildlife (bats), some of which may be transmissible to humans or specific to humans. In the human population, they can cause a wide range of diseases, mainly affecting the respiratory and digestive systems. In the scientific databases, there are huge numbers of research articles about the antiviral, antifungal, antibacterial, antiviral, and anthelmintic activities of medicinal herbs and crops with different ethnobotanical backgrounds. The subject of our research is the antiviral effect of isolated saponins, a purified saponin mixture, and a methanol extract of Astragalus glycyphyllos L. In the studies conducted for the cytotoxic effect of the substances, CC(50) (cytotoxic concentration 50) and MTC (maximum tolerable concentration) were determined by the colorimetric method (MTT assay). The virus was cultured in the MDBK cell line. As a result of the experiments carried out on the influence of substances on viral replication (using MTT-based colorimetric assay for detection of human Coronavirus replication inhibition), it was found that the extract and the purified saponin mixture inhibited 100% viral replication. The calculated selective indices are about 13 and 18, respectively. The obtained results make them promising for a preparation with anti-Coronavirus action. MDPI 2023-11-20 /pmc/articles/PMC10671514/ /pubmed/38003714 http://dx.doi.org/10.3390/ijms242216525 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hinkov, Anton
Tsvetkov, Venelin
Shkondrov, Aleksandar
Krasteva, Ilina
Shishkov, Stoyan
Shishkova, Kalina
Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title_full Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title_fullStr Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title_full_unstemmed Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title_short Effect of a Total Extract and Saponins from Astragalus glycyphyllos L. on Human Coronavirus Replication In Vitro
title_sort effect of a total extract and saponins from astragalus glycyphyllos l. on human coronavirus replication in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671514/
https://www.ncbi.nlm.nih.gov/pubmed/38003714
http://dx.doi.org/10.3390/ijms242216525
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