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FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats
Goat intramuscular fat (IMF) deposition is precisely regulated by many key genes as well as transcription factors. Nevertheless, the potential of the regulators of goat IMF deposition remains undefined. In this work, we reported that the transcription factor FOS is expressed at a low level at the ea...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671551/ https://www.ncbi.nlm.nih.gov/pubmed/38003034 http://dx.doi.org/10.3390/genes14112088 |
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author | Hu, Tingting Li, Zhibin Gong, Chengsi Xiong, Yan Sun, Shiyu Xing, Jiani Li, Yanyan Li, Ruiwen Wang, Youli Wang, Yong Lin, Yaqiu |
author_facet | Hu, Tingting Li, Zhibin Gong, Chengsi Xiong, Yan Sun, Shiyu Xing, Jiani Li, Yanyan Li, Ruiwen Wang, Youli Wang, Yong Lin, Yaqiu |
author_sort | Hu, Tingting |
collection | PubMed |
description | Goat intramuscular fat (IMF) deposition is precisely regulated by many key genes as well as transcription factors. Nevertheless, the potential of the regulators of goat IMF deposition remains undefined. In this work, we reported that the transcription factor FOS is expressed at a low level at the early differentiation stage and at a high level in late differentiation. The overexpression of FOS inhibited intramuscular adipocyte lipid accumulation and significantly downregulated the expressions of PPARγ, C/EBPβ, C/EBPα, AP2, SREBP1, FASN, ACC, HSL, and ATGL. Consistently, the knockdown of FOS, facilitated by two distinct siRNAs, significantly promoted intramuscular adipocyte lipid accumulation. Moreover, our analysis revealed multiple potential binding sites for FOS on the promoters of PPARγ, C/EBPβ, and C/EBPα. The expression changes in PPARγ, C/EBPβ, and C/EBPα during intramuscular adipogenesis were opposite to that of FOS. In summary, FOS inhibits intramuscular lipogenesis in goats and potentially negatively regulates the expressions of PPARγ, C/EBPβ, and C/EBPα genes. Our research will provide valuable data for the underlying molecular mechanism of the FOS regulation network of intramuscular lipogenesis. |
format | Online Article Text |
id | pubmed-10671551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106715512023-11-17 FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats Hu, Tingting Li, Zhibin Gong, Chengsi Xiong, Yan Sun, Shiyu Xing, Jiani Li, Yanyan Li, Ruiwen Wang, Youli Wang, Yong Lin, Yaqiu Genes (Basel) Article Goat intramuscular fat (IMF) deposition is precisely regulated by many key genes as well as transcription factors. Nevertheless, the potential of the regulators of goat IMF deposition remains undefined. In this work, we reported that the transcription factor FOS is expressed at a low level at the early differentiation stage and at a high level in late differentiation. The overexpression of FOS inhibited intramuscular adipocyte lipid accumulation and significantly downregulated the expressions of PPARγ, C/EBPβ, C/EBPα, AP2, SREBP1, FASN, ACC, HSL, and ATGL. Consistently, the knockdown of FOS, facilitated by two distinct siRNAs, significantly promoted intramuscular adipocyte lipid accumulation. Moreover, our analysis revealed multiple potential binding sites for FOS on the promoters of PPARγ, C/EBPβ, and C/EBPα. The expression changes in PPARγ, C/EBPβ, and C/EBPα during intramuscular adipogenesis were opposite to that of FOS. In summary, FOS inhibits intramuscular lipogenesis in goats and potentially negatively regulates the expressions of PPARγ, C/EBPβ, and C/EBPα genes. Our research will provide valuable data for the underlying molecular mechanism of the FOS regulation network of intramuscular lipogenesis. MDPI 2023-11-17 /pmc/articles/PMC10671551/ /pubmed/38003034 http://dx.doi.org/10.3390/genes14112088 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Tingting Li, Zhibin Gong, Chengsi Xiong, Yan Sun, Shiyu Xing, Jiani Li, Yanyan Li, Ruiwen Wang, Youli Wang, Yong Lin, Yaqiu FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title | FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title_full | FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title_fullStr | FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title_full_unstemmed | FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title_short | FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats |
title_sort | fos inhibits the differentiation of intramuscular adipocytes in goats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671551/ https://www.ncbi.nlm.nih.gov/pubmed/38003034 http://dx.doi.org/10.3390/genes14112088 |
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