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Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents

Smooth muscle tissue (SMT) is one of the main structural components of visceral organs, acting as a key factor in the development of adaptive and pathological conditions. Despite the crucial part of SMT in the gastrointestinal tract activity, the mechanisms of its gravisensitivity are still insuffic...

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Autores principales: Samoilenko, Tatyana, Shishkina, Viktoriya, Antakova, Lyubov, Goryushkina, Yelena, Kostin, Andrey, Buchwalow, Igor, Tiemann, Markus, Atiakshin, Dmitrii
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671600/
https://www.ncbi.nlm.nih.gov/pubmed/38003728
http://dx.doi.org/10.3390/ijms242216539
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author Samoilenko, Tatyana
Shishkina, Viktoriya
Antakova, Lyubov
Goryushkina, Yelena
Kostin, Andrey
Buchwalow, Igor
Tiemann, Markus
Atiakshin, Dmitrii
author_facet Samoilenko, Tatyana
Shishkina, Viktoriya
Antakova, Lyubov
Goryushkina, Yelena
Kostin, Andrey
Buchwalow, Igor
Tiemann, Markus
Atiakshin, Dmitrii
author_sort Samoilenko, Tatyana
collection PubMed
description Smooth muscle tissue (SMT) is one of the main structural components of visceral organs, acting as a key factor in the development of adaptive and pathological conditions. Despite the crucial part of SMT in the gastrointestinal tract activity, the mechanisms of its gravisensitivity are still insufficiently studied. The study evaluated the content of smooth muscle actin (α-SMA) in the membranes of the gastric fundus and jejunum in C57BL/6N mice (30-day space flight), in Mongolian gerbils Meriones unguiculatus (12-day orbital flight) and after anti-orthostatic suspension according to E.R. Morey-Holton. A morphometric analysis of α-SMA in the muscularis externa of the stomach and jejunum of mice and Mongolian gerbils from space flight groups revealed a decreased area of the immunopositive regions, a fact indicating a weakening of the SMT functional activity. Gravisensitivity of the contractile structures of the digestive system may be due to changes in the myofilament structural components of the smooth myocytes or myofibroblast actin. A simulated antiorthostatic suspension revealed no significant changes in the content of the α-SMA expression level, a fact supporting an alteration in the functional properties of the muscularis externa of the digestive hollow organs under weightless environment. The data obtained contribute to the novel mechanisms of the SMT contractile apparatus remodeling during orbital flights and can be used to improve preventive measures in space biomedicine.
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spelling pubmed-106716002023-11-20 Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents Samoilenko, Tatyana Shishkina, Viktoriya Antakova, Lyubov Goryushkina, Yelena Kostin, Andrey Buchwalow, Igor Tiemann, Markus Atiakshin, Dmitrii Int J Mol Sci Article Smooth muscle tissue (SMT) is one of the main structural components of visceral organs, acting as a key factor in the development of adaptive and pathological conditions. Despite the crucial part of SMT in the gastrointestinal tract activity, the mechanisms of its gravisensitivity are still insufficiently studied. The study evaluated the content of smooth muscle actin (α-SMA) in the membranes of the gastric fundus and jejunum in C57BL/6N mice (30-day space flight), in Mongolian gerbils Meriones unguiculatus (12-day orbital flight) and after anti-orthostatic suspension according to E.R. Morey-Holton. A morphometric analysis of α-SMA in the muscularis externa of the stomach and jejunum of mice and Mongolian gerbils from space flight groups revealed a decreased area of the immunopositive regions, a fact indicating a weakening of the SMT functional activity. Gravisensitivity of the contractile structures of the digestive system may be due to changes in the myofilament structural components of the smooth myocytes or myofibroblast actin. A simulated antiorthostatic suspension revealed no significant changes in the content of the α-SMA expression level, a fact supporting an alteration in the functional properties of the muscularis externa of the digestive hollow organs under weightless environment. The data obtained contribute to the novel mechanisms of the SMT contractile apparatus remodeling during orbital flights and can be used to improve preventive measures in space biomedicine. MDPI 2023-11-20 /pmc/articles/PMC10671600/ /pubmed/38003728 http://dx.doi.org/10.3390/ijms242216539 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Samoilenko, Tatyana
Shishkina, Viktoriya
Antakova, Lyubov
Goryushkina, Yelena
Kostin, Andrey
Buchwalow, Igor
Tiemann, Markus
Atiakshin, Dmitrii
Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title_full Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title_fullStr Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title_full_unstemmed Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title_short Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents
title_sort smooth muscle actin as a criterion for gravisensitivity of stomach and jejunum in laboratory rodents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671600/
https://www.ncbi.nlm.nih.gov/pubmed/38003728
http://dx.doi.org/10.3390/ijms242216539
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