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A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration

The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation...

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Autores principales: Humenik, Filip, Danko, Ján, Krešáková, Lenka, Vdoviaková, Katarína, Vrabec, Vladimír, Vasilová, Emília, Giretová, Mária, Tóth, Štefan, Fagová, Zuzana, Babík, Ján, Medvecký, Ľubomír
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671656/
https://www.ncbi.nlm.nih.gov/pubmed/38003269
http://dx.doi.org/10.3390/ijms242216080
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author Humenik, Filip
Danko, Ján
Krešáková, Lenka
Vdoviaková, Katarína
Vrabec, Vladimír
Vasilová, Emília
Giretová, Mária
Tóth, Štefan
Fagová, Zuzana
Babík, Ján
Medvecký, Ľubomír
author_facet Humenik, Filip
Danko, Ján
Krešáková, Lenka
Vdoviaková, Katarína
Vrabec, Vladimír
Vasilová, Emília
Giretová, Mária
Tóth, Štefan
Fagová, Zuzana
Babík, Ján
Medvecký, Ľubomír
author_sort Humenik, Filip
collection PubMed
description The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting.
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spelling pubmed-106716562023-11-08 A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration Humenik, Filip Danko, Ján Krešáková, Lenka Vdoviaková, Katarína Vrabec, Vladimír Vasilová, Emília Giretová, Mária Tóth, Štefan Fagová, Zuzana Babík, Ján Medvecký, Ľubomír Int J Mol Sci Article The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting. MDPI 2023-11-08 /pmc/articles/PMC10671656/ /pubmed/38003269 http://dx.doi.org/10.3390/ijms242216080 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Humenik, Filip
Danko, Ján
Krešáková, Lenka
Vdoviaková, Katarína
Vrabec, Vladimír
Vasilová, Emília
Giretová, Mária
Tóth, Štefan
Fagová, Zuzana
Babík, Ján
Medvecký, Ľubomír
A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title_full A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title_fullStr A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title_full_unstemmed A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title_short A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
title_sort chitosan-based biomaterial combined with mesenchymal stem cell-conditioned medium for wound healing and skin regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671656/
https://www.ncbi.nlm.nih.gov/pubmed/38003269
http://dx.doi.org/10.3390/ijms242216080
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