Cargando…
A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration
The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671656/ https://www.ncbi.nlm.nih.gov/pubmed/38003269 http://dx.doi.org/10.3390/ijms242216080 |
_version_ | 1785140209203019776 |
---|---|
author | Humenik, Filip Danko, Ján Krešáková, Lenka Vdoviaková, Katarína Vrabec, Vladimír Vasilová, Emília Giretová, Mária Tóth, Štefan Fagová, Zuzana Babík, Ján Medvecký, Ľubomír |
author_facet | Humenik, Filip Danko, Ján Krešáková, Lenka Vdoviaková, Katarína Vrabec, Vladimír Vasilová, Emília Giretová, Mária Tóth, Štefan Fagová, Zuzana Babík, Ján Medvecký, Ľubomír |
author_sort | Humenik, Filip |
collection | PubMed |
description | The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting. |
format | Online Article Text |
id | pubmed-10671656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106716562023-11-08 A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration Humenik, Filip Danko, Ján Krešáková, Lenka Vdoviaková, Katarína Vrabec, Vladimír Vasilová, Emília Giretová, Mária Tóth, Štefan Fagová, Zuzana Babík, Ján Medvecký, Ľubomír Int J Mol Sci Article The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting. MDPI 2023-11-08 /pmc/articles/PMC10671656/ /pubmed/38003269 http://dx.doi.org/10.3390/ijms242216080 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Humenik, Filip Danko, Ján Krešáková, Lenka Vdoviaková, Katarína Vrabec, Vladimír Vasilová, Emília Giretová, Mária Tóth, Štefan Fagová, Zuzana Babík, Ján Medvecký, Ľubomír A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title | A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title_full | A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title_fullStr | A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title_full_unstemmed | A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title_short | A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration |
title_sort | chitosan-based biomaterial combined with mesenchymal stem cell-conditioned medium for wound healing and skin regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671656/ https://www.ncbi.nlm.nih.gov/pubmed/38003269 http://dx.doi.org/10.3390/ijms242216080 |
work_keys_str_mv | AT humenikfilip achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT dankojan achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT kresakovalenka achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vdoviakovakatarina achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vrabecvladimir achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vasilovaemilia achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT giretovamaria achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT tothstefan achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT fagovazuzana achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT babikjan achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT medveckylubomir achitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT humenikfilip chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT dankojan chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT kresakovalenka chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vdoviakovakatarina chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vrabecvladimir chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT vasilovaemilia chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT giretovamaria chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT tothstefan chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT fagovazuzana chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT babikjan chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration AT medveckylubomir chitosanbasedbiomaterialcombinedwithmesenchymalstemcellconditionedmediumforwoundhealingandskinregeneration |