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Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized Electroporation

The introduction of plasmids into Agrobacterium cells is one of the key steps in the Agrobacterium-mediated transformation of plants for gene editing applications. Depending on chromosomal background, some Agrobacterium strains exhibit a very low transformation efficiency, which results in a low num...

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Detalles Bibliográficos
Autores principales: Liu, Xiang, Miceli, Joseph F., Patton, Sabrina, Murray, Melissa, Evans, John, Wei, Xiaoping, Wang, Pohao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671908/
https://www.ncbi.nlm.nih.gov/pubmed/38004357
http://dx.doi.org/10.3390/life13112217