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Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized Electroporation
The introduction of plasmids into Agrobacterium cells is one of the key steps in the Agrobacterium-mediated transformation of plants for gene editing applications. Depending on chromosomal background, some Agrobacterium strains exhibit a very low transformation efficiency, which results in a low num...
Autores principales: | Liu, Xiang, Miceli, Joseph F., Patton, Sabrina, Murray, Melissa, Evans, John, Wei, Xiaoping, Wang, Pohao |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671908/ https://www.ncbi.nlm.nih.gov/pubmed/38004357 http://dx.doi.org/10.3390/life13112217 |
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