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The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.

After crosses, the identification of true hybrids is not only the most important step in the initiation of a breeding program but also plays a crucial role in the improvement of hybrid varieties. However, current morphological or molecular-based hybrid identification methods are time-consuming and c...

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Autores principales: Sari, Hatice, Eker, Tuba, Sari, Duygu, Aksoy, Munevver, Bakır, Melike, Dogdu, Veysel, Toker, Cengiz, Canci, Huseyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672187/
https://www.ncbi.nlm.nih.gov/pubmed/38004362
http://dx.doi.org/10.3390/life13112222
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author Sari, Hatice
Eker, Tuba
Sari, Duygu
Aksoy, Munevver
Bakır, Melike
Dogdu, Veysel
Toker, Cengiz
Canci, Huseyin
author_facet Sari, Hatice
Eker, Tuba
Sari, Duygu
Aksoy, Munevver
Bakır, Melike
Dogdu, Veysel
Toker, Cengiz
Canci, Huseyin
author_sort Sari, Hatice
collection PubMed
description After crosses, the identification of true hybrids is not only the most important step in the initiation of a breeding program but also plays a crucial role in the improvement of hybrid varieties. However, current morphological or molecular-based hybrid identification methods are time-consuming and costly approaches that require knowledge and skill, as well as specific lab equipment. In the current study, xenia, direct or immediate effect of pollen on seeds was used to identify true hybrids in the genus Pisum L. for the first time without growing F(1) plants. The current study was therefore aimed to (i) elucidate the xenia effect on seeds in intra- and interspecific crosses between P. sativum L. subsp. sativum var. sativum or var. arvense L. Poir. and its wild relatives, including P. sativum subsp. elatius (M. Bieb.) Aschers & Graebn. and P. fulvum Sibth. & Sm., and (ii) illuminate the beneficialness of the xenia effect in a practical improvement of the genus Pisum L. The pea cultivars, including P. sativum subsp. sativum var. sativum and P. sativum subsp. sativum var. arvense, were therefore crossed with P. sativum subsp. elatius and P. fulvum, and the occurrence of the xenia effect was studied on the seeds of fertilized female plants immediately after the crosses. It was concluded that using the xenia effect for the early detection of true hybrid immediately after crossing was not only the fastest, most reliable, and least expensive option as early selection criteria, but that xenia also provided information about dominant seed and pod traits after double fertilization.
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spelling pubmed-106721872023-11-18 The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L. Sari, Hatice Eker, Tuba Sari, Duygu Aksoy, Munevver Bakır, Melike Dogdu, Veysel Toker, Cengiz Canci, Huseyin Life (Basel) Article After crosses, the identification of true hybrids is not only the most important step in the initiation of a breeding program but also plays a crucial role in the improvement of hybrid varieties. However, current morphological or molecular-based hybrid identification methods are time-consuming and costly approaches that require knowledge and skill, as well as specific lab equipment. In the current study, xenia, direct or immediate effect of pollen on seeds was used to identify true hybrids in the genus Pisum L. for the first time without growing F(1) plants. The current study was therefore aimed to (i) elucidate the xenia effect on seeds in intra- and interspecific crosses between P. sativum L. subsp. sativum var. sativum or var. arvense L. Poir. and its wild relatives, including P. sativum subsp. elatius (M. Bieb.) Aschers & Graebn. and P. fulvum Sibth. & Sm., and (ii) illuminate the beneficialness of the xenia effect in a practical improvement of the genus Pisum L. The pea cultivars, including P. sativum subsp. sativum var. sativum and P. sativum subsp. sativum var. arvense, were therefore crossed with P. sativum subsp. elatius and P. fulvum, and the occurrence of the xenia effect was studied on the seeds of fertilized female plants immediately after the crosses. It was concluded that using the xenia effect for the early detection of true hybrid immediately after crossing was not only the fastest, most reliable, and least expensive option as early selection criteria, but that xenia also provided information about dominant seed and pod traits after double fertilization. MDPI 2023-11-18 /pmc/articles/PMC10672187/ /pubmed/38004362 http://dx.doi.org/10.3390/life13112222 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sari, Hatice
Eker, Tuba
Sari, Duygu
Aksoy, Munevver
Bakır, Melike
Dogdu, Veysel
Toker, Cengiz
Canci, Huseyin
The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title_full The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title_fullStr The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title_full_unstemmed The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title_short The Fastest and Most Reliable Identification of True Hybrids in the Genus Pisum L.
title_sort fastest and most reliable identification of true hybrids in the genus pisum l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672187/
https://www.ncbi.nlm.nih.gov/pubmed/38004362
http://dx.doi.org/10.3390/life13112222
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