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Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats

Paracetamol or acetaminophen (PAC) is a commonly used analgesic and antipyretic drug. It has been shown that overdoses beyond the therapeutic range can cause hepatotoxicity and acute liver injury. The most common cause of drug-induced liver injury (DILI) in Saudi Arabia and worldwide is paracetamol...

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Autores principales: Koshak, Maimonah Fuad, El-Readi, Mahmoud Zaki, Elzubier, Mohamed Elzubier, Refaat, Bassem, Almaimani, Riyad Adnan, Idris, Shakir, Althubiti, Mohammad, Al-Amodi, Hiba Saeed, Eid, Safaa Yehia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672227/
https://www.ncbi.nlm.nih.gov/pubmed/37999416
http://dx.doi.org/10.3390/md21110592
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author Koshak, Maimonah Fuad
El-Readi, Mahmoud Zaki
Elzubier, Mohamed Elzubier
Refaat, Bassem
Almaimani, Riyad Adnan
Idris, Shakir
Althubiti, Mohammad
Al-Amodi, Hiba Saeed
Eid, Safaa Yehia
author_facet Koshak, Maimonah Fuad
El-Readi, Mahmoud Zaki
Elzubier, Mohamed Elzubier
Refaat, Bassem
Almaimani, Riyad Adnan
Idris, Shakir
Althubiti, Mohammad
Al-Amodi, Hiba Saeed
Eid, Safaa Yehia
author_sort Koshak, Maimonah Fuad
collection PubMed
description Paracetamol or acetaminophen (PAC) is a commonly used analgesic and antipyretic drug. It has been shown that overdoses beyond the therapeutic range can cause hepatotoxicity and acute liver injury. The most common cause of drug-induced liver injury (DILI) in Saudi Arabia and worldwide is paracetamol overdose. Fucoxanthin (FUC) is an allenic carotenoid that is found in edible brown seaweeds, and it has antioxidant and anti-inflammatory effects. Several studies have shown the potential therapeutic effects of FUC in diabetes, cancers, and inflammatory disorders. This study aims to investigate the protective effect of FUC against PAC-induced acute liver injury in rats. FUC was administered (100, 200, and 500 mg/kg, p.o.) for 7 days, and then the liver injury was induced by the administration of PAC (2000 mg/kg, oral). Blood and liver tissue samples were collected from PAC-positive untreated, treated, and negative control rats. Biochemical and inflammatory parameters in the blood were measured. In addition, RT-PCR, Western blotting, and immunohistochemistry were performed for liver tissue. The serum levels of liver biomarkers (ALT, AST, and ALP) increased after PAC-induced liver toxicity; FUC-treated rats showed lower levels compared to the positive control. There was an increase in the expression of TNF-α, IL-1, IL-6, NF-kB, INF-γ, and iNOS and a decrease in IL-10, IL-22, and IL-10R expression after the FUC treatment of injured liver rats. For the hepatic inflammation and PAC-toxicity-induced oxidative stress genes and proteins, FUC-treated rats (100, 200, and 500 mg/kg) showed a reduction in the expression of oxidative stress genes. These results showed that FUC protected the liver against PAC-induced injury through antioxidant and anti-inflammatory actions. However, further clinical studies are required to confirm the findings.
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spelling pubmed-106722272023-11-14 Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats Koshak, Maimonah Fuad El-Readi, Mahmoud Zaki Elzubier, Mohamed Elzubier Refaat, Bassem Almaimani, Riyad Adnan Idris, Shakir Althubiti, Mohammad Al-Amodi, Hiba Saeed Eid, Safaa Yehia Mar Drugs Article Paracetamol or acetaminophen (PAC) is a commonly used analgesic and antipyretic drug. It has been shown that overdoses beyond the therapeutic range can cause hepatotoxicity and acute liver injury. The most common cause of drug-induced liver injury (DILI) in Saudi Arabia and worldwide is paracetamol overdose. Fucoxanthin (FUC) is an allenic carotenoid that is found in edible brown seaweeds, and it has antioxidant and anti-inflammatory effects. Several studies have shown the potential therapeutic effects of FUC in diabetes, cancers, and inflammatory disorders. This study aims to investigate the protective effect of FUC against PAC-induced acute liver injury in rats. FUC was administered (100, 200, and 500 mg/kg, p.o.) for 7 days, and then the liver injury was induced by the administration of PAC (2000 mg/kg, oral). Blood and liver tissue samples were collected from PAC-positive untreated, treated, and negative control rats. Biochemical and inflammatory parameters in the blood were measured. In addition, RT-PCR, Western blotting, and immunohistochemistry were performed for liver tissue. The serum levels of liver biomarkers (ALT, AST, and ALP) increased after PAC-induced liver toxicity; FUC-treated rats showed lower levels compared to the positive control. There was an increase in the expression of TNF-α, IL-1, IL-6, NF-kB, INF-γ, and iNOS and a decrease in IL-10, IL-22, and IL-10R expression after the FUC treatment of injured liver rats. For the hepatic inflammation and PAC-toxicity-induced oxidative stress genes and proteins, FUC-treated rats (100, 200, and 500 mg/kg) showed a reduction in the expression of oxidative stress genes. These results showed that FUC protected the liver against PAC-induced injury through antioxidant and anti-inflammatory actions. However, further clinical studies are required to confirm the findings. MDPI 2023-11-14 /pmc/articles/PMC10672227/ /pubmed/37999416 http://dx.doi.org/10.3390/md21110592 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koshak, Maimonah Fuad
El-Readi, Mahmoud Zaki
Elzubier, Mohamed Elzubier
Refaat, Bassem
Almaimani, Riyad Adnan
Idris, Shakir
Althubiti, Mohammad
Al-Amodi, Hiba Saeed
Eid, Safaa Yehia
Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title_full Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title_fullStr Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title_full_unstemmed Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title_short Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
title_sort antioxidative and anti-inflammatory protective effects of fucoxanthin against paracetamol-induced hepatotoxicity in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672227/
https://www.ncbi.nlm.nih.gov/pubmed/37999416
http://dx.doi.org/10.3390/md21110592
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