Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line

Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrat...

Descripción completa

Detalles Bibliográficos
Autores principales: Kussainova, Assiya, Bulgakova, Olga, Aripova, Akmaral, Ibragimova, Milana, Pulliero, Alessandra, Begimbetova, Dinara, Bersimbaev, Rakhmetkazhi, Izzotti, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672232/
https://www.ncbi.nlm.nih.gov/pubmed/38003914
http://dx.doi.org/10.3390/jpm13111599
_version_ 1785140342883876864
author Kussainova, Assiya
Bulgakova, Olga
Aripova, Akmaral
Ibragimova, Milana
Pulliero, Alessandra
Begimbetova, Dinara
Bersimbaev, Rakhmetkazhi
Izzotti, Alberto
author_facet Kussainova, Assiya
Bulgakova, Olga
Aripova, Akmaral
Ibragimova, Milana
Pulliero, Alessandra
Begimbetova, Dinara
Bersimbaev, Rakhmetkazhi
Izzotti, Alberto
author_sort Kussainova, Assiya
collection PubMed
description Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.
format Online
Article
Text
id pubmed-10672232
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106722322023-11-12 Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line Kussainova, Assiya Bulgakova, Olga Aripova, Akmaral Ibragimova, Milana Pulliero, Alessandra Begimbetova, Dinara Bersimbaev, Rakhmetkazhi Izzotti, Alberto J Pers Med Article Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos. MDPI 2023-11-12 /pmc/articles/PMC10672232/ /pubmed/38003914 http://dx.doi.org/10.3390/jpm13111599 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kussainova, Assiya
Bulgakova, Olga
Aripova, Akmaral
Ibragimova, Milana
Pulliero, Alessandra
Begimbetova, Dinara
Bersimbaev, Rakhmetkazhi
Izzotti, Alberto
Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title_full Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title_fullStr Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title_full_unstemmed Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title_short Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
title_sort molecular and cellular mechanism of action of chrysotile asbestos in mrc5 cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672232/
https://www.ncbi.nlm.nih.gov/pubmed/38003914
http://dx.doi.org/10.3390/jpm13111599
work_keys_str_mv AT kussainovaassiya molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT bulgakovaolga molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT aripovaakmaral molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT ibragimovamilana molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT pullieroalessandra molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT begimbetovadinara molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT bersimbaevrakhmetkazhi molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline
AT izzottialberto molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline