Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672232/ https://www.ncbi.nlm.nih.gov/pubmed/38003914 http://dx.doi.org/10.3390/jpm13111599 |
_version_ | 1785140342883876864 |
---|---|
author | Kussainova, Assiya Bulgakova, Olga Aripova, Akmaral Ibragimova, Milana Pulliero, Alessandra Begimbetova, Dinara Bersimbaev, Rakhmetkazhi Izzotti, Alberto |
author_facet | Kussainova, Assiya Bulgakova, Olga Aripova, Akmaral Ibragimova, Milana Pulliero, Alessandra Begimbetova, Dinara Bersimbaev, Rakhmetkazhi Izzotti, Alberto |
author_sort | Kussainova, Assiya |
collection | PubMed |
description | Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos. |
format | Online Article Text |
id | pubmed-10672232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106722322023-11-12 Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line Kussainova, Assiya Bulgakova, Olga Aripova, Akmaral Ibragimova, Milana Pulliero, Alessandra Begimbetova, Dinara Bersimbaev, Rakhmetkazhi Izzotti, Alberto J Pers Med Article Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos. MDPI 2023-11-12 /pmc/articles/PMC10672232/ /pubmed/38003914 http://dx.doi.org/10.3390/jpm13111599 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kussainova, Assiya Bulgakova, Olga Aripova, Akmaral Ibragimova, Milana Pulliero, Alessandra Begimbetova, Dinara Bersimbaev, Rakhmetkazhi Izzotti, Alberto Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title | Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title_full | Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title_fullStr | Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title_full_unstemmed | Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title_short | Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line |
title_sort | molecular and cellular mechanism of action of chrysotile asbestos in mrc5 cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672232/ https://www.ncbi.nlm.nih.gov/pubmed/38003914 http://dx.doi.org/10.3390/jpm13111599 |
work_keys_str_mv | AT kussainovaassiya molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT bulgakovaolga molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT aripovaakmaral molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT ibragimovamilana molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT pullieroalessandra molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT begimbetovadinara molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT bersimbaevrakhmetkazhi molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline AT izzottialberto molecularandcellularmechanismofactionofchrysotileasbestosinmrc5cellline |