UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity

The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H(2)O(2)-degraded polysaccharides from Sargassum fusiforme. Results indicated that two fractions with different monosaccharide compositions and morphologic...

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Autores principales: Chang, Shiyuan, Chen, Xiaoyong, Chen, Yifan, You, Lijun, Hileuskaya, Kseniya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672335/
https://www.ncbi.nlm.nih.gov/pubmed/37999385
http://dx.doi.org/10.3390/md21110561
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author Chang, Shiyuan
Chen, Xiaoyong
Chen, Yifan
You, Lijun
Hileuskaya, Kseniya
author_facet Chang, Shiyuan
Chen, Xiaoyong
Chen, Yifan
You, Lijun
Hileuskaya, Kseniya
author_sort Chang, Shiyuan
collection PubMed
description The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H(2)O(2)-degraded polysaccharides from Sargassum fusiforme. Results indicated that two fractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW 264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fucp, t-Manp, 6-Galp, t-Fucp, and 3,4-GlcAp. These results would provide theoretical support for studying the structural properties and biological activities of UV/H(2)O(2)-degraded polysaccharides.
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spelling pubmed-106723352023-10-26 UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity Chang, Shiyuan Chen, Xiaoyong Chen, Yifan You, Lijun Hileuskaya, Kseniya Mar Drugs Article The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H(2)O(2)-degraded polysaccharides from Sargassum fusiforme. Results indicated that two fractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW 264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fucp, t-Manp, 6-Galp, t-Fucp, and 3,4-GlcAp. These results would provide theoretical support for studying the structural properties and biological activities of UV/H(2)O(2)-degraded polysaccharides. MDPI 2023-10-26 /pmc/articles/PMC10672335/ /pubmed/37999385 http://dx.doi.org/10.3390/md21110561 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Shiyuan
Chen, Xiaoyong
Chen, Yifan
You, Lijun
Hileuskaya, Kseniya
UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title_full UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title_fullStr UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title_full_unstemmed UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title_short UV/H(2)O(2)-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
title_sort uv/h(2)o(2)-degraded polysaccharides from sargassum fusiforme: purification, structural properties, and anti-inflammatory activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672335/
https://www.ncbi.nlm.nih.gov/pubmed/37999385
http://dx.doi.org/10.3390/md21110561
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