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The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia

The Zizania latifolia is usually infected by the obligate parasitic fungus Ustilago esculenta to form an edible fleshy stem which is an aquatic vegetable called Jiaobai in China. The infection by the teliospore (T) strain of U. esculenta induces Z. latifolia forming gray fleshy stems, while the myce...

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Autores principales: Li, Yipeng, Hu, Cailin, Song, Ruiqi, Yin, Zhihui, Wang, Lingyun, Shi, Lin, Li, Wei, Zheng, Zhaisheng, Yang, Mengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672487/
https://www.ncbi.nlm.nih.gov/pubmed/37998872
http://dx.doi.org/10.3390/jof9111067
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author Li, Yipeng
Hu, Cailin
Song, Ruiqi
Yin, Zhihui
Wang, Lingyun
Shi, Lin
Li, Wei
Zheng, Zhaisheng
Yang, Mengfei
author_facet Li, Yipeng
Hu, Cailin
Song, Ruiqi
Yin, Zhihui
Wang, Lingyun
Shi, Lin
Li, Wei
Zheng, Zhaisheng
Yang, Mengfei
author_sort Li, Yipeng
collection PubMed
description The Zizania latifolia is usually infected by the obligate parasitic fungus Ustilago esculenta to form an edible fleshy stem which is an aquatic vegetable called Jiaobai in China. The infection by the teliospore (T) strain of U. esculenta induces Z. latifolia forming gray fleshy stems, while the mycelia-teliospore (MT) strain of U. esculenta induces white fleshy stems which are more suitable for edibility than gray fleshy stems. The mechanism of this phenomenon is still largely unknown. One of the possible causes is the diversity of endophytic microbial communities between these two fleshy stems. Therefore, we utilized fungal ITS1 and bacterial 16S rDNA amplicon sequencing to investigate the diversity of endophytic microbial communities in the two different fleshy stems of Z. latifolia. The results revealed that the α diversity and richness of endophytic fungi in white Z. latifolia were significantly greater than in gray Z. latifolia. The dominant fungal genus in both fleshy stems was U. esculenta, which accounted for over 90% of the endophytic fungi. The community composition of endophytic fungi in gray and white Z. latifolia was different except for U. esculenta, and a negative correlation was observed between U. esculenta and other endophytic fungi. In addition, the dominant bacterial genus in gray Z. latifolia was Alcaligenaceae which is also negatively correlated with other bacterium communities. Additionally, the co-occurrence network of white Z. latifolia was found to have a stronger scale, connectivity, and complexity compared to that of gray Z. latifolia. And the detected beneficial bacteria and pathogens in the stems of Z. latifolia potentially compete for resources. Furthermore, the function of endophytic bacteria is more abundant than endophytic fungi in Z. latifolia. This research investigated the correlation between the development of Z. latifolia fleshy stems and endophytic microbial communities. Our findings indicate that the composition of endophytic microbial communities is closely related to the type of Z. latifolia fleshy stems. This research also suggests the potential utilization of specific microbial communities to enhance the growth and development of Z. latifolia, thereby contributing to the breeding of Z. latifolia.
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spelling pubmed-106724872023-11-01 The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia Li, Yipeng Hu, Cailin Song, Ruiqi Yin, Zhihui Wang, Lingyun Shi, Lin Li, Wei Zheng, Zhaisheng Yang, Mengfei J Fungi (Basel) Article The Zizania latifolia is usually infected by the obligate parasitic fungus Ustilago esculenta to form an edible fleshy stem which is an aquatic vegetable called Jiaobai in China. The infection by the teliospore (T) strain of U. esculenta induces Z. latifolia forming gray fleshy stems, while the mycelia-teliospore (MT) strain of U. esculenta induces white fleshy stems which are more suitable for edibility than gray fleshy stems. The mechanism of this phenomenon is still largely unknown. One of the possible causes is the diversity of endophytic microbial communities between these two fleshy stems. Therefore, we utilized fungal ITS1 and bacterial 16S rDNA amplicon sequencing to investigate the diversity of endophytic microbial communities in the two different fleshy stems of Z. latifolia. The results revealed that the α diversity and richness of endophytic fungi in white Z. latifolia were significantly greater than in gray Z. latifolia. The dominant fungal genus in both fleshy stems was U. esculenta, which accounted for over 90% of the endophytic fungi. The community composition of endophytic fungi in gray and white Z. latifolia was different except for U. esculenta, and a negative correlation was observed between U. esculenta and other endophytic fungi. In addition, the dominant bacterial genus in gray Z. latifolia was Alcaligenaceae which is also negatively correlated with other bacterium communities. Additionally, the co-occurrence network of white Z. latifolia was found to have a stronger scale, connectivity, and complexity compared to that of gray Z. latifolia. And the detected beneficial bacteria and pathogens in the stems of Z. latifolia potentially compete for resources. Furthermore, the function of endophytic bacteria is more abundant than endophytic fungi in Z. latifolia. This research investigated the correlation between the development of Z. latifolia fleshy stems and endophytic microbial communities. Our findings indicate that the composition of endophytic microbial communities is closely related to the type of Z. latifolia fleshy stems. This research also suggests the potential utilization of specific microbial communities to enhance the growth and development of Z. latifolia, thereby contributing to the breeding of Z. latifolia. MDPI 2023-11-01 /pmc/articles/PMC10672487/ /pubmed/37998872 http://dx.doi.org/10.3390/jof9111067 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yipeng
Hu, Cailin
Song, Ruiqi
Yin, Zhihui
Wang, Lingyun
Shi, Lin
Li, Wei
Zheng, Zhaisheng
Yang, Mengfei
The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title_full The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title_fullStr The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title_full_unstemmed The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title_short The Difference in Diversity between Endophytic Microorganisms in White and Grey Zizania latifolia
title_sort difference in diversity between endophytic microorganisms in white and grey zizania latifolia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672487/
https://www.ncbi.nlm.nih.gov/pubmed/37998872
http://dx.doi.org/10.3390/jof9111067
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