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Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care

This research work aims to develop functional toothpastes with combined enamel remineralization and antibacterial effects using nano-hydroxyapatites (nHAPs) and birch extract. Eleven toothpastes (notated as P1–P11) were designed featuring different concentrations of birch extract and a constant conc...

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Autores principales: Florea, Alexandra-Diana, Dobrota, Cristina Teodora, Carpa, Rahela, Racz, Csaba-Pal, Tomoaia, Gheorghe, Mocanu, Aurora, Avram, Alexandra, Soritau, Olga, Pop, Lucian Cristian, Tomoaia-Cotisel, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672495/
https://www.ncbi.nlm.nih.gov/pubmed/38005073
http://dx.doi.org/10.3390/ma16227143
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author Florea, Alexandra-Diana
Dobrota, Cristina Teodora
Carpa, Rahela
Racz, Csaba-Pal
Tomoaia, Gheorghe
Mocanu, Aurora
Avram, Alexandra
Soritau, Olga
Pop, Lucian Cristian
Tomoaia-Cotisel, Maria
author_facet Florea, Alexandra-Diana
Dobrota, Cristina Teodora
Carpa, Rahela
Racz, Csaba-Pal
Tomoaia, Gheorghe
Mocanu, Aurora
Avram, Alexandra
Soritau, Olga
Pop, Lucian Cristian
Tomoaia-Cotisel, Maria
author_sort Florea, Alexandra-Diana
collection PubMed
description This research work aims to develop functional toothpastes with combined enamel remineralization and antibacterial effects using nano-hydroxyapatites (nHAPs) and birch extract. Eleven toothpastes (notated as P1–P11) were designed featuring different concentrations of birch extract and a constant concentration of pure nHAPs or substituted nHAPs (HAP-5%Zn, HAP-0.23%Mg-3.9%Zn-2%Si-10%Sr, and HAP-2.5%Mg-2.9%Si-1.34%Zn). In vitro assessments involved treating artificially demineralized enamel slices and analyzing surface repair and remineralization using Atomic Force Microscopy (AFM). The Agar Disk Diffusion method was used to measure antibacterial activity against Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis, Streptococcus mutans, and Staphylococcus aureus. Topographic images of enamel structure and surface roughness, as well as the ability of nHAP nanoparticles to form self-assembled layers, revealed excellent restorative properties of the tested toothpastes, with enamel nanostructure normalization occurring as soon as 10 days after treatment. The outcomes highlighted enamel morphology improvements due to the toothpaste treatment also having various efficacious antibacterial effects. Promising results were obtained using P5 toothpaste, containing HAP-5%Zn (3.4%) and birch extract (1.3%), indicating notable remineralization and good antibacterial properties. This study represents a significant advancement in oral care by introducing toothpaste formulations that simultaneously promote enamel health through effective remineralization and bacterial inhibition.
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spelling pubmed-106724952023-11-13 Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care Florea, Alexandra-Diana Dobrota, Cristina Teodora Carpa, Rahela Racz, Csaba-Pal Tomoaia, Gheorghe Mocanu, Aurora Avram, Alexandra Soritau, Olga Pop, Lucian Cristian Tomoaia-Cotisel, Maria Materials (Basel) Article This research work aims to develop functional toothpastes with combined enamel remineralization and antibacterial effects using nano-hydroxyapatites (nHAPs) and birch extract. Eleven toothpastes (notated as P1–P11) were designed featuring different concentrations of birch extract and a constant concentration of pure nHAPs or substituted nHAPs (HAP-5%Zn, HAP-0.23%Mg-3.9%Zn-2%Si-10%Sr, and HAP-2.5%Mg-2.9%Si-1.34%Zn). In vitro assessments involved treating artificially demineralized enamel slices and analyzing surface repair and remineralization using Atomic Force Microscopy (AFM). The Agar Disk Diffusion method was used to measure antibacterial activity against Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis, Streptococcus mutans, and Staphylococcus aureus. Topographic images of enamel structure and surface roughness, as well as the ability of nHAP nanoparticles to form self-assembled layers, revealed excellent restorative properties of the tested toothpastes, with enamel nanostructure normalization occurring as soon as 10 days after treatment. The outcomes highlighted enamel morphology improvements due to the toothpaste treatment also having various efficacious antibacterial effects. Promising results were obtained using P5 toothpaste, containing HAP-5%Zn (3.4%) and birch extract (1.3%), indicating notable remineralization and good antibacterial properties. This study represents a significant advancement in oral care by introducing toothpaste formulations that simultaneously promote enamel health through effective remineralization and bacterial inhibition. MDPI 2023-11-13 /pmc/articles/PMC10672495/ /pubmed/38005073 http://dx.doi.org/10.3390/ma16227143 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Florea, Alexandra-Diana
Dobrota, Cristina Teodora
Carpa, Rahela
Racz, Csaba-Pal
Tomoaia, Gheorghe
Mocanu, Aurora
Avram, Alexandra
Soritau, Olga
Pop, Lucian Cristian
Tomoaia-Cotisel, Maria
Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title_full Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title_fullStr Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title_full_unstemmed Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title_short Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
title_sort optimization of functional toothpaste formulation containing nano-hydroxyapatite and birch extract for daily oral care
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672495/
https://www.ncbi.nlm.nih.gov/pubmed/38005073
http://dx.doi.org/10.3390/ma16227143
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