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In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues

Previous research has reported on hidden damage within the dentin introduced by cryopreservation, but the effect on the mechanical properties of the hard tissues at tooth level remains unclear. The main objective of this study is to investigate the effect of cryopreservation on the mechanical proper...

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Autores principales: De Roo, Noëmi M. C., Toulouse, Kaat, Thierens, Laurent A. M., Henry, Silke, De Buyser, Stefanie, Temmerman, Liesbeth, Verbeeck, Ronald M. H., De Pauw, Guy A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672544/
https://www.ncbi.nlm.nih.gov/pubmed/37998120
http://dx.doi.org/10.3390/jfb14110551
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author De Roo, Noëmi M. C.
Toulouse, Kaat
Thierens, Laurent A. M.
Henry, Silke
De Buyser, Stefanie
Temmerman, Liesbeth
Verbeeck, Ronald M. H.
De Pauw, Guy A. M.
author_facet De Roo, Noëmi M. C.
Toulouse, Kaat
Thierens, Laurent A. M.
Henry, Silke
De Buyser, Stefanie
Temmerman, Liesbeth
Verbeeck, Ronald M. H.
De Pauw, Guy A. M.
author_sort De Roo, Noëmi M. C.
collection PubMed
description Previous research has reported on hidden damage within the dentin introduced by cryopreservation, but the effect on the mechanical properties of the hard tissues at tooth level remains unclear. The main objective of this study is to investigate the effect of cryopreservation on the mechanical properties of teeth. A matched sample of 234 premolars of 117 children (9 ≤ age ≤ 16 years), bilaterally extracted for orthodontic reasons, were included. For each child, one tooth was randomly allocated to the cryopreservation group and the contralateral tooth was assigned to the control group. Static compression tests were performed to determine load to failure, stiffness, and toughness. In a subgroup of 20 teeth, a cyclic preloading or chewing simulation was performed. Additionally, the fracture mode was determined, and the microstructure of the fractured surfaces was examined using a scanning electron microscope (SEM). Linear mixed model analyses could not detect a statistical difference in the mean load to failure (p = 0.549), mean toughness (p = 0.968), or mean stiffness (p = 0.150) between cryopreserved and non-cryopreserved teeth. No significant difference in load to failure after cyclic preloading was detected between groups (p = 0.734). SEM analysis revealed comparable fracture characteristics between groups. It is concluded that cryopreservation does not affect the mean load to failure, stiffness, or toughness of teeth, indicating that hidden damage in the dentin is not critical at tooth level.
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spelling pubmed-106725442023-11-17 In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues De Roo, Noëmi M. C. Toulouse, Kaat Thierens, Laurent A. M. Henry, Silke De Buyser, Stefanie Temmerman, Liesbeth Verbeeck, Ronald M. H. De Pauw, Guy A. M. J Funct Biomater Article Previous research has reported on hidden damage within the dentin introduced by cryopreservation, but the effect on the mechanical properties of the hard tissues at tooth level remains unclear. The main objective of this study is to investigate the effect of cryopreservation on the mechanical properties of teeth. A matched sample of 234 premolars of 117 children (9 ≤ age ≤ 16 years), bilaterally extracted for orthodontic reasons, were included. For each child, one tooth was randomly allocated to the cryopreservation group and the contralateral tooth was assigned to the control group. Static compression tests were performed to determine load to failure, stiffness, and toughness. In a subgroup of 20 teeth, a cyclic preloading or chewing simulation was performed. Additionally, the fracture mode was determined, and the microstructure of the fractured surfaces was examined using a scanning electron microscope (SEM). Linear mixed model analyses could not detect a statistical difference in the mean load to failure (p = 0.549), mean toughness (p = 0.968), or mean stiffness (p = 0.150) between cryopreserved and non-cryopreserved teeth. No significant difference in load to failure after cyclic preloading was detected between groups (p = 0.734). SEM analysis revealed comparable fracture characteristics between groups. It is concluded that cryopreservation does not affect the mean load to failure, stiffness, or toughness of teeth, indicating that hidden damage in the dentin is not critical at tooth level. MDPI 2023-11-17 /pmc/articles/PMC10672544/ /pubmed/37998120 http://dx.doi.org/10.3390/jfb14110551 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Roo, Noëmi M. C.
Toulouse, Kaat
Thierens, Laurent A. M.
Henry, Silke
De Buyser, Stefanie
Temmerman, Liesbeth
Verbeeck, Ronald M. H.
De Pauw, Guy A. M.
In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title_full In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title_fullStr In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title_full_unstemmed In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title_short In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues
title_sort in vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672544/
https://www.ncbi.nlm.nih.gov/pubmed/37998120
http://dx.doi.org/10.3390/jfb14110551
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