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Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells
This study aimed to investigate the optimal conditions for Papanicolaou (Pap) smear to increase the success rate of target cell isolation through manual microdissection (MMD) and prevent cell spread. Pap smears were prepared using an HPV42-positive SurePath™ liquid-based cytology case, and 46 and 50...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672744/ https://www.ncbi.nlm.nih.gov/pubmed/38004711 http://dx.doi.org/10.3390/microorganisms11112700 |
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author | Okayama, Kaori Kakinuma, Mao Tajima, Saki Nagasawa, Norika Ishii, Yasuyoshi Oda, Mizue Kimura, Hirokazu Okodo, Mitsuaki |
author_facet | Okayama, Kaori Kakinuma, Mao Tajima, Saki Nagasawa, Norika Ishii, Yasuyoshi Oda, Mizue Kimura, Hirokazu Okodo, Mitsuaki |
author_sort | Okayama, Kaori |
collection | PubMed |
description | This study aimed to investigate the optimal conditions for Papanicolaou (Pap) smear to increase the success rate of target cell isolation through manual microdissection (MMD) and prevent cell spread. Pap smears were prepared using an HPV42-positive SurePath™ liquid-based cytology case, and 46 and 50 koilocytes were used in wet and dried Pap smears, respectively, to verify the success rate of target cell isolation using MMD based on the HPV detection rate. During MMD, the microscopic examination of both specimens revealed that cells in dried smears could be easily identified; however, cell debris remained in the surrounding area after MMD. Although it was difficult to observe cells in wet smears, there was no cell debris. When the needle tip was immersed in DNA lysate after cell isolation through MMD, a difference in cell solubility was found between dry and wet smears. HPV42 was detected in 94.7% and 97.4% of dried and wet Pap smears, respectively, via polymerase chain reaction genotyping using lysed cell solution; the detection rates were not significantly different. The isolation of target cells from wet Pap smears using MMD reduced the risk of contamination and increased the success rate of HPV detection. This study might facilitate the identification of new CIN-derived HPV-infected cells using MMD with wet Pap smears. |
format | Online Article Text |
id | pubmed-10672744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106727442023-11-03 Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells Okayama, Kaori Kakinuma, Mao Tajima, Saki Nagasawa, Norika Ishii, Yasuyoshi Oda, Mizue Kimura, Hirokazu Okodo, Mitsuaki Microorganisms Communication This study aimed to investigate the optimal conditions for Papanicolaou (Pap) smear to increase the success rate of target cell isolation through manual microdissection (MMD) and prevent cell spread. Pap smears were prepared using an HPV42-positive SurePath™ liquid-based cytology case, and 46 and 50 koilocytes were used in wet and dried Pap smears, respectively, to verify the success rate of target cell isolation using MMD based on the HPV detection rate. During MMD, the microscopic examination of both specimens revealed that cells in dried smears could be easily identified; however, cell debris remained in the surrounding area after MMD. Although it was difficult to observe cells in wet smears, there was no cell debris. When the needle tip was immersed in DNA lysate after cell isolation through MMD, a difference in cell solubility was found between dry and wet smears. HPV42 was detected in 94.7% and 97.4% of dried and wet Pap smears, respectively, via polymerase chain reaction genotyping using lysed cell solution; the detection rates were not significantly different. The isolation of target cells from wet Pap smears using MMD reduced the risk of contamination and increased the success rate of HPV detection. This study might facilitate the identification of new CIN-derived HPV-infected cells using MMD with wet Pap smears. MDPI 2023-11-03 /pmc/articles/PMC10672744/ /pubmed/38004711 http://dx.doi.org/10.3390/microorganisms11112700 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Okayama, Kaori Kakinuma, Mao Tajima, Saki Nagasawa, Norika Ishii, Yasuyoshi Oda, Mizue Kimura, Hirokazu Okodo, Mitsuaki Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title | Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title_full | Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title_fullStr | Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title_full_unstemmed | Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title_short | Optimal Papanicolaou Smear Conditions for Manual Microdissection of Single Target Cells |
title_sort | optimal papanicolaou smear conditions for manual microdissection of single target cells |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672744/ https://www.ncbi.nlm.nih.gov/pubmed/38004711 http://dx.doi.org/10.3390/microorganisms11112700 |
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