Cargando…

Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E

Repurposing vitamins as antiviral supporting agents is a rapid approach used to control emerging viral infections. Although there is considerable evidence supporting the use of vitamin supplementation in viral infections, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Moatasim, Yassmin, Kutkat, Omnia, Osman, Ahmed M., Gomaa, Mokhtar R., Okda, Faten, El Sayes, Mohamed, Kamel, Mina Nabil, Gaballah, Mohamed, Mostafa, Ahmed, El-Shesheny, Rabeh, Kayali, Ghazi, Ali, Mohamed A., Kandeil, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673013/
https://www.ncbi.nlm.nih.gov/pubmed/38004788
http://dx.doi.org/10.3390/microorganisms11112777
_version_ 1785149571547004928
author Moatasim, Yassmin
Kutkat, Omnia
Osman, Ahmed M.
Gomaa, Mokhtar R.
Okda, Faten
El Sayes, Mohamed
Kamel, Mina Nabil
Gaballah, Mohamed
Mostafa, Ahmed
El-Shesheny, Rabeh
Kayali, Ghazi
Ali, Mohamed A.
Kandeil, Ahmed
author_facet Moatasim, Yassmin
Kutkat, Omnia
Osman, Ahmed M.
Gomaa, Mokhtar R.
Okda, Faten
El Sayes, Mohamed
Kamel, Mina Nabil
Gaballah, Mohamed
Mostafa, Ahmed
El-Shesheny, Rabeh
Kayali, Ghazi
Ali, Mohamed A.
Kandeil, Ahmed
author_sort Moatasim, Yassmin
collection PubMed
description Repurposing vitamins as antiviral supporting agents is a rapid approach used to control emerging viral infections. Although there is considerable evidence supporting the use of vitamin supplementation in viral infections, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the specific role of each vitamin in defending against coronaviruses remains unclear. Antiviral activities of available vitamins on the infectivity and replication of human coronaviruses, namely, SARS-CoV-2, Middle East respiratory syndrome coronavirus (MERS-CoV), and human coronavirus 229E (HCoV-229E), were investigated using in silico and in vitro studies. We identified potential broad-spectrum inhibitor effects of Hydroxocobalamin and Methylcobalamin against the three tested CoVs. Cyanocobalamin could selectively affect SARS-CoV-2 but not MERS-CoV and HCoV-229E. Methylcobalamin showed significantly higher inhibition values on SARS-CoV-2 compared with Hydroxocobalamin and Cyanocobalamin, while Hydroxocobalamin showed the highest potent antiviral activity against MERS-CoV and Cyanocobalamin against HCoV-229E. Furthermore, in silico studies were performed for these promising vitamins to investigate their interaction with SARS-CoV-2, MERS-CoV, and HCoV-229E viral-specific cell receptors (ACE2, DPP4, and hAPN protein, respectively) and viral proteins (S-RBD, 3CL pro, RdRp), suggesting that Hydroxocobalamin, Methylcobalamin, and Cyanocobalamin may have significant binding affinity to these proteins. These results show that Methylcobalamin may have potential benefits for coronavirus-infected patients.
format Online
Article
Text
id pubmed-10673013
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106730132023-11-15 Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E Moatasim, Yassmin Kutkat, Omnia Osman, Ahmed M. Gomaa, Mokhtar R. Okda, Faten El Sayes, Mohamed Kamel, Mina Nabil Gaballah, Mohamed Mostafa, Ahmed El-Shesheny, Rabeh Kayali, Ghazi Ali, Mohamed A. Kandeil, Ahmed Microorganisms Article Repurposing vitamins as antiviral supporting agents is a rapid approach used to control emerging viral infections. Although there is considerable evidence supporting the use of vitamin supplementation in viral infections, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the specific role of each vitamin in defending against coronaviruses remains unclear. Antiviral activities of available vitamins on the infectivity and replication of human coronaviruses, namely, SARS-CoV-2, Middle East respiratory syndrome coronavirus (MERS-CoV), and human coronavirus 229E (HCoV-229E), were investigated using in silico and in vitro studies. We identified potential broad-spectrum inhibitor effects of Hydroxocobalamin and Methylcobalamin against the three tested CoVs. Cyanocobalamin could selectively affect SARS-CoV-2 but not MERS-CoV and HCoV-229E. Methylcobalamin showed significantly higher inhibition values on SARS-CoV-2 compared with Hydroxocobalamin and Cyanocobalamin, while Hydroxocobalamin showed the highest potent antiviral activity against MERS-CoV and Cyanocobalamin against HCoV-229E. Furthermore, in silico studies were performed for these promising vitamins to investigate their interaction with SARS-CoV-2, MERS-CoV, and HCoV-229E viral-specific cell receptors (ACE2, DPP4, and hAPN protein, respectively) and viral proteins (S-RBD, 3CL pro, RdRp), suggesting that Hydroxocobalamin, Methylcobalamin, and Cyanocobalamin may have significant binding affinity to these proteins. These results show that Methylcobalamin may have potential benefits for coronavirus-infected patients. MDPI 2023-11-15 /pmc/articles/PMC10673013/ /pubmed/38004788 http://dx.doi.org/10.3390/microorganisms11112777 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moatasim, Yassmin
Kutkat, Omnia
Osman, Ahmed M.
Gomaa, Mokhtar R.
Okda, Faten
El Sayes, Mohamed
Kamel, Mina Nabil
Gaballah, Mohamed
Mostafa, Ahmed
El-Shesheny, Rabeh
Kayali, Ghazi
Ali, Mohamed A.
Kandeil, Ahmed
Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title_full Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title_fullStr Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title_full_unstemmed Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title_short Potent Antiviral Activity of Vitamin B12 against Severe Acute Respiratory Syndrome Coronavirus 2, Middle East Respiratory Syndrome Coronavirus, and Human Coronavirus 229E
title_sort potent antiviral activity of vitamin b12 against severe acute respiratory syndrome coronavirus 2, middle east respiratory syndrome coronavirus, and human coronavirus 229e
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673013/
https://www.ncbi.nlm.nih.gov/pubmed/38004788
http://dx.doi.org/10.3390/microorganisms11112777
work_keys_str_mv AT moatasimyassmin potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT kutkatomnia potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT osmanahmedm potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT gomaamokhtarr potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT okdafaten potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT elsayesmohamed potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT kamelminanabil potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT gaballahmohamed potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT mostafaahmed potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT elsheshenyrabeh potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT kayalighazi potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT alimohameda potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e
AT kandeilahmed potentantiviralactivityofvitaminb12againstsevereacuterespiratorysyndromecoronavirus2middleeastrespiratorysyndromecoronavirusandhumancoronavirus229e