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Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound

An investigation into the mechanisms of action on bacteria involving exposure to stress factors was conducted in this study. The effects of ultrasound on Escherichia coli K-12 MG1655 and its isogenic mutant, ∆gadW, under high power ultrasound treatments (26 kHz) were screened and identified by analy...

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Autores principales: Spiteri, David, Griffin, Sholeem, Karatzas, Kimon Andreas, Scerri, Christian, Valdramidis, Vasilis P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673019/
https://www.ncbi.nlm.nih.gov/pubmed/38004779
http://dx.doi.org/10.3390/microorganisms11112768
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author Spiteri, David
Griffin, Sholeem
Karatzas, Kimon Andreas
Scerri, Christian
Valdramidis, Vasilis P.
author_facet Spiteri, David
Griffin, Sholeem
Karatzas, Kimon Andreas
Scerri, Christian
Valdramidis, Vasilis P.
author_sort Spiteri, David
collection PubMed
description An investigation into the mechanisms of action on bacteria involving exposure to stress factors was conducted in this study. The effects of ultrasound on Escherichia coli K-12 MG1655 and its isogenic mutant, ∆gadW, under high power ultrasound treatments (26 kHz) were screened and identified by analysing their transcriptome differences between primary and secondary sequential treatments using RNA-Seq. This also helped to assess any developed protection for cells between different generations. According to our results, 1825 genes of all tested conditions were expressed, playing different roles in the cell. The expression of these genes is associated with DNA damage, cell membrane integrity, and also metabolic effects. The studied strains also showed different differential expressed genes (DEGs), with some genes being directly responsible for defence mechanisms, while others play an indirect effect due to cell damage. A gradual decrease in the expression of the genes, as we moved from just one cycle of ultrasound treatment to sequential treatment, was evident from a heat map analysis of the results. Overall, E. coli K-12 builds a self-protection mechanism by increasing the expression of genes involved in the respiration for increased growth, and production of flagellum and pili. It can be concluded that high power ultrasound is a technology that triggers several different defence mechanisms which directly link to E. coli.
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spelling pubmed-106730192023-11-14 Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound Spiteri, David Griffin, Sholeem Karatzas, Kimon Andreas Scerri, Christian Valdramidis, Vasilis P. Microorganisms Article An investigation into the mechanisms of action on bacteria involving exposure to stress factors was conducted in this study. The effects of ultrasound on Escherichia coli K-12 MG1655 and its isogenic mutant, ∆gadW, under high power ultrasound treatments (26 kHz) were screened and identified by analysing their transcriptome differences between primary and secondary sequential treatments using RNA-Seq. This also helped to assess any developed protection for cells between different generations. According to our results, 1825 genes of all tested conditions were expressed, playing different roles in the cell. The expression of these genes is associated with DNA damage, cell membrane integrity, and also metabolic effects. The studied strains also showed different differential expressed genes (DEGs), with some genes being directly responsible for defence mechanisms, while others play an indirect effect due to cell damage. A gradual decrease in the expression of the genes, as we moved from just one cycle of ultrasound treatment to sequential treatment, was evident from a heat map analysis of the results. Overall, E. coli K-12 builds a self-protection mechanism by increasing the expression of genes involved in the respiration for increased growth, and production of flagellum and pili. It can be concluded that high power ultrasound is a technology that triggers several different defence mechanisms which directly link to E. coli. MDPI 2023-11-14 /pmc/articles/PMC10673019/ /pubmed/38004779 http://dx.doi.org/10.3390/microorganisms11112768 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spiteri, David
Griffin, Sholeem
Karatzas, Kimon Andreas
Scerri, Christian
Valdramidis, Vasilis P.
Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title_full Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title_fullStr Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title_full_unstemmed Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title_short Escherichia coli K-12 Transcriptomics for Assessing the Mechanism of Action of High-Power Ultrasound
title_sort escherichia coli k-12 transcriptomics for assessing the mechanism of action of high-power ultrasound
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673019/
https://www.ncbi.nlm.nih.gov/pubmed/38004779
http://dx.doi.org/10.3390/microorganisms11112768
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