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Postmortem Alteration of Purine Metabolism in Coronary Artery Disease

A new approach for assisting in the diagnosis of coronary artery disease (CAD) as a cause of death is essential in cases where complete autopsy examinations are not feasible. The purine pathway has been associated with CAD patients, but the understanding of this pathway in postmortem changes needs t...

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Autores principales: Somtua, Phakchira, Jaikang, Churdsak, Konguthaithip, Giatgong, Intui, Kanicnan, Watcharakhom, Somlada, O’Brien, Timothy E., Amornlertwatana, Yutti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673240/
https://www.ncbi.nlm.nih.gov/pubmed/37999231
http://dx.doi.org/10.3390/metabo13111135
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author Somtua, Phakchira
Jaikang, Churdsak
Konguthaithip, Giatgong
Intui, Kanicnan
Watcharakhom, Somlada
O’Brien, Timothy E.
Amornlertwatana, Yutti
author_facet Somtua, Phakchira
Jaikang, Churdsak
Konguthaithip, Giatgong
Intui, Kanicnan
Watcharakhom, Somlada
O’Brien, Timothy E.
Amornlertwatana, Yutti
author_sort Somtua, Phakchira
collection PubMed
description A new approach for assisting in the diagnosis of coronary artery disease (CAD) as a cause of death is essential in cases where complete autopsy examinations are not feasible. The purine pathway has been associated with CAD patients, but the understanding of this pathway in postmortem changes needs to be explored. This study investigated the levels of blood purine metabolites in CAD after death. Heart blood samples (n = 60) were collected and divided into CAD (n = 23) and control groups (n = 37). Purine metabolites were measured via proton nuclear magnetic resonance. Guanosine triphosphate (GTP), nicotinamide adenine dinucleotide (NAD), and xanthine levels significantly decreased (p < 0.05); conversely, adenine and deoxyribose 5-phosphate levels significantly increased (p < 0.05) in the CAD group compared to the control group. Decreasing xanthine levels may serve as a marker for predicting the cause of death in CAD (AUC = 0.7). Our findings suggest that the purine pathway was interrupted by physiological processes after death, causing the metabolism of the deceased to differ from that of the living. Additionally, xanthine levels should be studied further to better understand their relationship with CAD and used as a biomarker for CAD diagnosis under decomposition and skeletonization settings.
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spelling pubmed-106732402023-11-08 Postmortem Alteration of Purine Metabolism in Coronary Artery Disease Somtua, Phakchira Jaikang, Churdsak Konguthaithip, Giatgong Intui, Kanicnan Watcharakhom, Somlada O’Brien, Timothy E. Amornlertwatana, Yutti Metabolites Article A new approach for assisting in the diagnosis of coronary artery disease (CAD) as a cause of death is essential in cases where complete autopsy examinations are not feasible. The purine pathway has been associated with CAD patients, but the understanding of this pathway in postmortem changes needs to be explored. This study investigated the levels of blood purine metabolites in CAD after death. Heart blood samples (n = 60) were collected and divided into CAD (n = 23) and control groups (n = 37). Purine metabolites were measured via proton nuclear magnetic resonance. Guanosine triphosphate (GTP), nicotinamide adenine dinucleotide (NAD), and xanthine levels significantly decreased (p < 0.05); conversely, adenine and deoxyribose 5-phosphate levels significantly increased (p < 0.05) in the CAD group compared to the control group. Decreasing xanthine levels may serve as a marker for predicting the cause of death in CAD (AUC = 0.7). Our findings suggest that the purine pathway was interrupted by physiological processes after death, causing the metabolism of the deceased to differ from that of the living. Additionally, xanthine levels should be studied further to better understand their relationship with CAD and used as a biomarker for CAD diagnosis under decomposition and skeletonization settings. MDPI 2023-11-08 /pmc/articles/PMC10673240/ /pubmed/37999231 http://dx.doi.org/10.3390/metabo13111135 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Somtua, Phakchira
Jaikang, Churdsak
Konguthaithip, Giatgong
Intui, Kanicnan
Watcharakhom, Somlada
O’Brien, Timothy E.
Amornlertwatana, Yutti
Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title_full Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title_fullStr Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title_full_unstemmed Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title_short Postmortem Alteration of Purine Metabolism in Coronary Artery Disease
title_sort postmortem alteration of purine metabolism in coronary artery disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673240/
https://www.ncbi.nlm.nih.gov/pubmed/37999231
http://dx.doi.org/10.3390/metabo13111135
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