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Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea

Major clinical symptoms of Theileria infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including Theileria in Korea, only a few have focused on Theileria infection in deer, including the Korean wa...

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Autores principales: Chung, Chang Uk, Lee, Haeseung, Seo, Min-Goo, Lee, Seung-Hun, Kim, Kyoo-Tae, Nazim, Kaifa, Song, Jung-Sun, Bae, Dong Hwa, Rhee, Man Hee, Kwon, Oh-Deog, Kwak, Dongmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673458/
https://www.ncbi.nlm.nih.gov/pubmed/38004751
http://dx.doi.org/10.3390/microorganisms11112740
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author Chung, Chang Uk
Lee, Haeseung
Seo, Min-Goo
Lee, Seung-Hun
Kim, Kyoo-Tae
Nazim, Kaifa
Song, Jung-Sun
Bae, Dong Hwa
Rhee, Man Hee
Kwon, Oh-Deog
Kwak, Dongmi
author_facet Chung, Chang Uk
Lee, Haeseung
Seo, Min-Goo
Lee, Seung-Hun
Kim, Kyoo-Tae
Nazim, Kaifa
Song, Jung-Sun
Bae, Dong Hwa
Rhee, Man Hee
Kwon, Oh-Deog
Kwak, Dongmi
author_sort Chung, Chang Uk
collection PubMed
description Major clinical symptoms of Theileria infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including Theileria in Korea, only a few have focused on Theileria infection in deer, including the Korean water deer. Blood samples from 160 deer were collected and subjected to DNA extraction and polymerase chain reaction (PCR). Next, PCR-positive samples were sequenced and analyzed by constructing a phylogenetic tree. The results showed that the overall infection rate of Theileria was 8.1% (13/160). Infection rates of 100% were observed in the northern and southern regions. However, the study’s limitation was its small sample size, wherein five and one samples were analyzed from the northern and southern regions, respectively. The central region exhibited the lowest infection rate of 2.9% (4/140). Infection rates also differed based on seasons, with the highest (18.4%, 9/49) being observed in spring, followed by that in summer (8.9%, 4/45). However, no infection was observed during autumn and winter. A phylogenetic analysis indicated that the PCR-positive samples contained Theileria luwenshuni, which usually infects small ruminants, such as goats and sheep.
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spelling pubmed-106734582023-11-09 Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea Chung, Chang Uk Lee, Haeseung Seo, Min-Goo Lee, Seung-Hun Kim, Kyoo-Tae Nazim, Kaifa Song, Jung-Sun Bae, Dong Hwa Rhee, Man Hee Kwon, Oh-Deog Kwak, Dongmi Microorganisms Article Major clinical symptoms of Theileria infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including Theileria in Korea, only a few have focused on Theileria infection in deer, including the Korean water deer. Blood samples from 160 deer were collected and subjected to DNA extraction and polymerase chain reaction (PCR). Next, PCR-positive samples were sequenced and analyzed by constructing a phylogenetic tree. The results showed that the overall infection rate of Theileria was 8.1% (13/160). Infection rates of 100% were observed in the northern and southern regions. However, the study’s limitation was its small sample size, wherein five and one samples were analyzed from the northern and southern regions, respectively. The central region exhibited the lowest infection rate of 2.9% (4/140). Infection rates also differed based on seasons, with the highest (18.4%, 9/49) being observed in spring, followed by that in summer (8.9%, 4/45). However, no infection was observed during autumn and winter. A phylogenetic analysis indicated that the PCR-positive samples contained Theileria luwenshuni, which usually infects small ruminants, such as goats and sheep. MDPI 2023-11-09 /pmc/articles/PMC10673458/ /pubmed/38004751 http://dx.doi.org/10.3390/microorganisms11112740 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chung, Chang Uk
Lee, Haeseung
Seo, Min-Goo
Lee, Seung-Hun
Kim, Kyoo-Tae
Nazim, Kaifa
Song, Jung-Sun
Bae, Dong Hwa
Rhee, Man Hee
Kwon, Oh-Deog
Kwak, Dongmi
Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title_full Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title_fullStr Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title_full_unstemmed Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title_short Molecular Detection and Genotyping of Theileria spp. in Deer (Cervidae) in Korea
title_sort molecular detection and genotyping of theileria spp. in deer (cervidae) in korea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673458/
https://www.ncbi.nlm.nih.gov/pubmed/38004751
http://dx.doi.org/10.3390/microorganisms11112740
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