Cargando…
Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts
The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant (Ribes nigrum L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1),...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673464/ https://www.ncbi.nlm.nih.gov/pubmed/38005180 http://dx.doi.org/10.3390/molecules28227459 |
_version_ | 1785140628864106496 |
---|---|
author | Staszowska-Karkut, Monika Chilczuk, Barbara Materska, Małgorzata Kontek, Renata Marciniak, Beata |
author_facet | Staszowska-Karkut, Monika Chilczuk, Barbara Materska, Małgorzata Kontek, Renata Marciniak, Beata |
author_sort | Staszowska-Karkut, Monika |
collection | PubMed |
description | The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant (Ribes nigrum L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH(•) and ABTS(+•)), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) cancer cells. Phenolics were identified using HPLC-QTOF-MS, and twelve compounds were quantified by HPLC-DAD. Finally, principal component analysis was carried out to assess the relationship between the tested factors. The results confirmed that blackcurrant leaves are a rich source of phenolics with high antioxidant activity and anticancer properties. It was demonstrated that the F2 fraction had the highest content of phenolics and the highest antiradical activity. Additionally, only this fraction showed cytotoxic activity against HCT 116 cells. It was confirmed that both the blackcurrant leaf extract and its fractions are a promising source of condensed active compounds and can be used as natural functional food additives. |
format | Online Article Text |
id | pubmed-10673464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106734642023-11-07 Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts Staszowska-Karkut, Monika Chilczuk, Barbara Materska, Małgorzata Kontek, Renata Marciniak, Beata Molecules Article The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant (Ribes nigrum L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH(•) and ABTS(+•)), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) cancer cells. Phenolics were identified using HPLC-QTOF-MS, and twelve compounds were quantified by HPLC-DAD. Finally, principal component analysis was carried out to assess the relationship between the tested factors. The results confirmed that blackcurrant leaves are a rich source of phenolics with high antioxidant activity and anticancer properties. It was demonstrated that the F2 fraction had the highest content of phenolics and the highest antiradical activity. Additionally, only this fraction showed cytotoxic activity against HCT 116 cells. It was confirmed that both the blackcurrant leaf extract and its fractions are a promising source of condensed active compounds and can be used as natural functional food additives. MDPI 2023-11-07 /pmc/articles/PMC10673464/ /pubmed/38005180 http://dx.doi.org/10.3390/molecules28227459 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Staszowska-Karkut, Monika Chilczuk, Barbara Materska, Małgorzata Kontek, Renata Marciniak, Beata Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title | Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title_full | Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title_fullStr | Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title_full_unstemmed | Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title_short | Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts |
title_sort | phenolic compounds in fractionated blackcurrant leaf extracts in relation to the biological activity of the extracts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673464/ https://www.ncbi.nlm.nih.gov/pubmed/38005180 http://dx.doi.org/10.3390/molecules28227459 |
work_keys_str_mv | AT staszowskakarkutmonika phenoliccompoundsinfractionatedblackcurrantleafextractsinrelationtothebiologicalactivityoftheextracts AT chilczukbarbara phenoliccompoundsinfractionatedblackcurrantleafextractsinrelationtothebiologicalactivityoftheextracts AT materskamałgorzata phenoliccompoundsinfractionatedblackcurrantleafextractsinrelationtothebiologicalactivityoftheextracts AT kontekrenata phenoliccompoundsinfractionatedblackcurrantleafextractsinrelationtothebiologicalactivityoftheextracts AT marciniakbeata phenoliccompoundsinfractionatedblackcurrantleafextractsinrelationtothebiologicalactivityoftheextracts |