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Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describ...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673465/ https://www.ncbi.nlm.nih.gov/pubmed/38004773 http://dx.doi.org/10.3390/microorganisms11112762 |
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author | Sierra-López, Francisco Castelan-Ramírez, Ismael Hernández-Martínez, Dolores Salazar-Villatoro, Lizbeth Segura-Cobos, David Flores-Maldonado, Catalina Hernández-Ramírez, Verónica Ivonne Villamar-Duque, Tomás Ernesto Méndez-Cruz, Adolfo René Talamás-Rohana, Patricia Omaña-Molina, Maritza |
author_facet | Sierra-López, Francisco Castelan-Ramírez, Ismael Hernández-Martínez, Dolores Salazar-Villatoro, Lizbeth Segura-Cobos, David Flores-Maldonado, Catalina Hernández-Ramírez, Verónica Ivonne Villamar-Duque, Tomás Ernesto Méndez-Cruz, Adolfo René Talamás-Rohana, Patricia Omaña-Molina, Maritza |
author_sort | Sierra-López, Francisco |
collection | PubMed |
description | Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describing the pathogenic processes carried out by these protozoans more precisely, we considered it important to determine the emission of extracellular vesicles (EVs) as part of the contact-independent pathogenicity mechanisms of A. culbertsoni, a highly pathogenic strain. Through transmission electronic microscopy (TEM) and nanoparticle tracking analysis (NTA), EVs were characterized. EVs showed lipid membrane and a size between 60 and 855 nm. The secretion of large vesicles was corroborated by confocal and TEM microscopy. The SDS-PAGE of EVs showed proteins of 45 to 200 kDa. Antigenic recognition was determined by Western Blot, and the internalization of EVs by trophozoites was observed through Dil-labeled EVs. In addition, some EVs biological characteristics were determined, such as proteolytic, hemolytic and COX activity. Furthermore, we highlighted the presence of leishmanolysin in trophozites and EVs. These results suggest that EVs are part of a contact-independent mechanism, which, together with contact-dependent ones, allow for a better understanding of the pathogenicity carried out by Acanthamoeba culbertsoni. |
format | Online Article Text |
id | pubmed-10673465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106734652023-11-14 Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis Sierra-López, Francisco Castelan-Ramírez, Ismael Hernández-Martínez, Dolores Salazar-Villatoro, Lizbeth Segura-Cobos, David Flores-Maldonado, Catalina Hernández-Ramírez, Verónica Ivonne Villamar-Duque, Tomás Ernesto Méndez-Cruz, Adolfo René Talamás-Rohana, Patricia Omaña-Molina, Maritza Microorganisms Article Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describing the pathogenic processes carried out by these protozoans more precisely, we considered it important to determine the emission of extracellular vesicles (EVs) as part of the contact-independent pathogenicity mechanisms of A. culbertsoni, a highly pathogenic strain. Through transmission electronic microscopy (TEM) and nanoparticle tracking analysis (NTA), EVs were characterized. EVs showed lipid membrane and a size between 60 and 855 nm. The secretion of large vesicles was corroborated by confocal and TEM microscopy. The SDS-PAGE of EVs showed proteins of 45 to 200 kDa. Antigenic recognition was determined by Western Blot, and the internalization of EVs by trophozoites was observed through Dil-labeled EVs. In addition, some EVs biological characteristics were determined, such as proteolytic, hemolytic and COX activity. Furthermore, we highlighted the presence of leishmanolysin in trophozites and EVs. These results suggest that EVs are part of a contact-independent mechanism, which, together with contact-dependent ones, allow for a better understanding of the pathogenicity carried out by Acanthamoeba culbertsoni. MDPI 2023-11-14 /pmc/articles/PMC10673465/ /pubmed/38004773 http://dx.doi.org/10.3390/microorganisms11112762 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sierra-López, Francisco Castelan-Ramírez, Ismael Hernández-Martínez, Dolores Salazar-Villatoro, Lizbeth Segura-Cobos, David Flores-Maldonado, Catalina Hernández-Ramírez, Verónica Ivonne Villamar-Duque, Tomás Ernesto Méndez-Cruz, Adolfo René Talamás-Rohana, Patricia Omaña-Molina, Maritza Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title | Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title_full | Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title_fullStr | Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title_full_unstemmed | Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title_short | Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis |
title_sort | extracellular vesicles secreted by acanthamoeba culbertsoni have cox and proteolytic activity and induce hemolysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673465/ https://www.ncbi.nlm.nih.gov/pubmed/38004773 http://dx.doi.org/10.3390/microorganisms11112762 |
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