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Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis

Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describ...

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Autores principales: Sierra-López, Francisco, Castelan-Ramírez, Ismael, Hernández-Martínez, Dolores, Salazar-Villatoro, Lizbeth, Segura-Cobos, David, Flores-Maldonado, Catalina, Hernández-Ramírez, Verónica Ivonne, Villamar-Duque, Tomás Ernesto, Méndez-Cruz, Adolfo René, Talamás-Rohana, Patricia, Omaña-Molina, Maritza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673465/
https://www.ncbi.nlm.nih.gov/pubmed/38004773
http://dx.doi.org/10.3390/microorganisms11112762
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author Sierra-López, Francisco
Castelan-Ramírez, Ismael
Hernández-Martínez, Dolores
Salazar-Villatoro, Lizbeth
Segura-Cobos, David
Flores-Maldonado, Catalina
Hernández-Ramírez, Verónica Ivonne
Villamar-Duque, Tomás Ernesto
Méndez-Cruz, Adolfo René
Talamás-Rohana, Patricia
Omaña-Molina, Maritza
author_facet Sierra-López, Francisco
Castelan-Ramírez, Ismael
Hernández-Martínez, Dolores
Salazar-Villatoro, Lizbeth
Segura-Cobos, David
Flores-Maldonado, Catalina
Hernández-Ramírez, Verónica Ivonne
Villamar-Duque, Tomás Ernesto
Méndez-Cruz, Adolfo René
Talamás-Rohana, Patricia
Omaña-Molina, Maritza
author_sort Sierra-López, Francisco
collection PubMed
description Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describing the pathogenic processes carried out by these protozoans more precisely, we considered it important to determine the emission of extracellular vesicles (EVs) as part of the contact-independent pathogenicity mechanisms of A. culbertsoni, a highly pathogenic strain. Through transmission electronic microscopy (TEM) and nanoparticle tracking analysis (NTA), EVs were characterized. EVs showed lipid membrane and a size between 60 and 855 nm. The secretion of large vesicles was corroborated by confocal and TEM microscopy. The SDS-PAGE of EVs showed proteins of 45 to 200 kDa. Antigenic recognition was determined by Western Blot, and the internalization of EVs by trophozoites was observed through Dil-labeled EVs. In addition, some EVs biological characteristics were determined, such as proteolytic, hemolytic and COX activity. Furthermore, we highlighted the presence of leishmanolysin in trophozites and EVs. These results suggest that EVs are part of a contact-independent mechanism, which, together with contact-dependent ones, allow for a better understanding of the pathogenicity carried out by Acanthamoeba culbertsoni.
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spelling pubmed-106734652023-11-14 Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis Sierra-López, Francisco Castelan-Ramírez, Ismael Hernández-Martínez, Dolores Salazar-Villatoro, Lizbeth Segura-Cobos, David Flores-Maldonado, Catalina Hernández-Ramírez, Verónica Ivonne Villamar-Duque, Tomás Ernesto Méndez-Cruz, Adolfo René Talamás-Rohana, Patricia Omaña-Molina, Maritza Microorganisms Article Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describing the pathogenic processes carried out by these protozoans more precisely, we considered it important to determine the emission of extracellular vesicles (EVs) as part of the contact-independent pathogenicity mechanisms of A. culbertsoni, a highly pathogenic strain. Through transmission electronic microscopy (TEM) and nanoparticle tracking analysis (NTA), EVs were characterized. EVs showed lipid membrane and a size between 60 and 855 nm. The secretion of large vesicles was corroborated by confocal and TEM microscopy. The SDS-PAGE of EVs showed proteins of 45 to 200 kDa. Antigenic recognition was determined by Western Blot, and the internalization of EVs by trophozoites was observed through Dil-labeled EVs. In addition, some EVs biological characteristics were determined, such as proteolytic, hemolytic and COX activity. Furthermore, we highlighted the presence of leishmanolysin in trophozites and EVs. These results suggest that EVs are part of a contact-independent mechanism, which, together with contact-dependent ones, allow for a better understanding of the pathogenicity carried out by Acanthamoeba culbertsoni. MDPI 2023-11-14 /pmc/articles/PMC10673465/ /pubmed/38004773 http://dx.doi.org/10.3390/microorganisms11112762 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sierra-López, Francisco
Castelan-Ramírez, Ismael
Hernández-Martínez, Dolores
Salazar-Villatoro, Lizbeth
Segura-Cobos, David
Flores-Maldonado, Catalina
Hernández-Ramírez, Verónica Ivonne
Villamar-Duque, Tomás Ernesto
Méndez-Cruz, Adolfo René
Talamás-Rohana, Patricia
Omaña-Molina, Maritza
Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title_full Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title_fullStr Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title_full_unstemmed Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title_short Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis
title_sort extracellular vesicles secreted by acanthamoeba culbertsoni have cox and proteolytic activity and induce hemolysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673465/
https://www.ncbi.nlm.nih.gov/pubmed/38004773
http://dx.doi.org/10.3390/microorganisms11112762
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