Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products

Quantum dot nanobeads (QBs) were used as signal source to develop competitive lateral flow immunoassay (LFIA) for the detection of chloramphenicol (CAP). The quantitative detection of CAP was achieved by calculating the total color difference (∆E) values of the test line (T line) using the images of...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Qian, Fan, Ling, Liu, Xiuying, Tang, Yiwei, Wang, Pingping, Shu, Zaixi, Zhang, Wei, Zhu, Lijie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673565/
https://www.ncbi.nlm.nih.gov/pubmed/38005218
http://dx.doi.org/10.3390/molecules28227496
_version_ 1785140652687753216
author Han, Qian
Fan, Ling
Liu, Xiuying
Tang, Yiwei
Wang, Pingping
Shu, Zaixi
Zhang, Wei
Zhu, Lijie
author_facet Han, Qian
Fan, Ling
Liu, Xiuying
Tang, Yiwei
Wang, Pingping
Shu, Zaixi
Zhang, Wei
Zhu, Lijie
author_sort Han, Qian
collection PubMed
description Quantum dot nanobeads (QBs) were used as signal source to develop competitive lateral flow immunoassay (LFIA) for the detection of chloramphenicol (CAP). The quantitative detection of CAP was achieved by calculating the total color difference (∆E) values of the test line (T line) using the images of test strips. QB-based LFIA (QBs-LFIA) allowed the effective dynamic linear detection of CAP in the range of 0.1–1.5 ng/mL. The limit of detection (LOD) was 3.0 ng/mL, which was 50 and 667 times lower than those achieved for two different brands of colloidal gold kits. The recoveries of CAP during real-sample detection were 82.82–104.91% at spiked levels of 0.1, 0.7, and 1.5 ng/mL. These results indicate that the developed QBs-LFIA facilitates the sensitive detection of CAP.
format Online
Article
Text
id pubmed-10673565
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106735652023-11-09 Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products Han, Qian Fan, Ling Liu, Xiuying Tang, Yiwei Wang, Pingping Shu, Zaixi Zhang, Wei Zhu, Lijie Molecules Article Quantum dot nanobeads (QBs) were used as signal source to develop competitive lateral flow immunoassay (LFIA) for the detection of chloramphenicol (CAP). The quantitative detection of CAP was achieved by calculating the total color difference (∆E) values of the test line (T line) using the images of test strips. QB-based LFIA (QBs-LFIA) allowed the effective dynamic linear detection of CAP in the range of 0.1–1.5 ng/mL. The limit of detection (LOD) was 3.0 ng/mL, which was 50 and 667 times lower than those achieved for two different brands of colloidal gold kits. The recoveries of CAP during real-sample detection were 82.82–104.91% at spiked levels of 0.1, 0.7, and 1.5 ng/mL. These results indicate that the developed QBs-LFIA facilitates the sensitive detection of CAP. MDPI 2023-11-09 /pmc/articles/PMC10673565/ /pubmed/38005218 http://dx.doi.org/10.3390/molecules28227496 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Qian
Fan, Ling
Liu, Xiuying
Tang, Yiwei
Wang, Pingping
Shu, Zaixi
Zhang, Wei
Zhu, Lijie
Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title_full Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title_fullStr Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title_full_unstemmed Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title_short Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products
title_sort lateral flow immunoassay based on quantum-dot nanobeads for detection of chloramphenicol in aquatic products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673565/
https://www.ncbi.nlm.nih.gov/pubmed/38005218
http://dx.doi.org/10.3390/molecules28227496
work_keys_str_mv AT hanqian lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT fanling lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT liuxiuying lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT tangyiwei lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT wangpingping lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT shuzaixi lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT zhangwei lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts
AT zhulijie lateralflowimmunoassaybasedonquantumdotnanobeadsfordetectionofchloramphenicolinaquaticproducts

Ejemplares similares