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Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells

Radiotherapy, in which X-rays are commonly used, is one of the most effective procedures for treating cancer. However, some cancer cells become resistant to radiation therapy, leading to poor prognosis. Therefore, a new therapeutic method is required to prevent cancer cells from acquiring radiation...

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Autores principales: Ito, Hiromu, Shoji, Yoshimi, Ueno, Megumi, Matsumoto, Ken-ichiro, Nakanishi, Ikuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674178/
https://www.ncbi.nlm.nih.gov/pubmed/38004516
http://dx.doi.org/10.3390/pharmaceutics15112536
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author Ito, Hiromu
Shoji, Yoshimi
Ueno, Megumi
Matsumoto, Ken-ichiro
Nakanishi, Ikuo
author_facet Ito, Hiromu
Shoji, Yoshimi
Ueno, Megumi
Matsumoto, Ken-ichiro
Nakanishi, Ikuo
author_sort Ito, Hiromu
collection PubMed
description Radiotherapy, in which X-rays are commonly used, is one of the most effective procedures for treating cancer. However, some cancer cells become resistant to radiation therapy, leading to poor prognosis. Therefore, a new therapeutic method is required to prevent cancer cells from acquiring radiation resistance. Photodynamic therapy (PDT) is a cancer treatment that uses photosensitizers, such as porphyrin compounds, and low-powered laser irradiation. We previously reported that reactive oxygen species (ROS) derived from mitochondria induce the expression of a porphyrin transporter (HCP1) and that laser irradiation enhances the cytotoxic effect. In addition, X-ray irradiation induces the production of mitochondrial ROS. Therefore, radioresistant cancer cells established with continuous X-ray irradiation would also overexpress ROS, and photodynamic therapy could be an effective therapeutic method. In this study, we established radioresistant cancer cells and examined the therapeutic effects and mechanisms with photodynamic therapy. We confirmed that X-ray-resistant cells showed overgeneration of mitochondrial ROS and elevated expression of HCP1, which led to the active accumulation of porphyrin and an increase in cytotoxicity with laser irradiation. Thus, photodynamic therapy is a promising treatment for X-ray-resistant cancers.
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spelling pubmed-106741782023-10-26 Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells Ito, Hiromu Shoji, Yoshimi Ueno, Megumi Matsumoto, Ken-ichiro Nakanishi, Ikuo Pharmaceutics Article Radiotherapy, in which X-rays are commonly used, is one of the most effective procedures for treating cancer. However, some cancer cells become resistant to radiation therapy, leading to poor prognosis. Therefore, a new therapeutic method is required to prevent cancer cells from acquiring radiation resistance. Photodynamic therapy (PDT) is a cancer treatment that uses photosensitizers, such as porphyrin compounds, and low-powered laser irradiation. We previously reported that reactive oxygen species (ROS) derived from mitochondria induce the expression of a porphyrin transporter (HCP1) and that laser irradiation enhances the cytotoxic effect. In addition, X-ray irradiation induces the production of mitochondrial ROS. Therefore, radioresistant cancer cells established with continuous X-ray irradiation would also overexpress ROS, and photodynamic therapy could be an effective therapeutic method. In this study, we established radioresistant cancer cells and examined the therapeutic effects and mechanisms with photodynamic therapy. We confirmed that X-ray-resistant cells showed overgeneration of mitochondrial ROS and elevated expression of HCP1, which led to the active accumulation of porphyrin and an increase in cytotoxicity with laser irradiation. Thus, photodynamic therapy is a promising treatment for X-ray-resistant cancers. MDPI 2023-10-26 /pmc/articles/PMC10674178/ /pubmed/38004516 http://dx.doi.org/10.3390/pharmaceutics15112536 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ito, Hiromu
Shoji, Yoshimi
Ueno, Megumi
Matsumoto, Ken-ichiro
Nakanishi, Ikuo
Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title_full Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title_fullStr Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title_full_unstemmed Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title_short Photodynamic Therapy for X-ray-Induced Radiation-Resistant Cancer Cells
title_sort photodynamic therapy for x-ray-induced radiation-resistant cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674178/
https://www.ncbi.nlm.nih.gov/pubmed/38004516
http://dx.doi.org/10.3390/pharmaceutics15112536
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