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An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection

Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS)...

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Autores principales: Skakic, Ivana, Francis, Jasmine E., Dekiwadia, Chaitali, Aibinu, Ibukun, Huq, Mohsina, Taki, Aya C., Walduck, Anna, Smooker, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674275/
https://www.ncbi.nlm.nih.gov/pubmed/38005984
http://dx.doi.org/10.3390/vaccines11111652
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author Skakic, Ivana
Francis, Jasmine E.
Dekiwadia, Chaitali
Aibinu, Ibukun
Huq, Mohsina
Taki, Aya C.
Walduck, Anna
Smooker, Peter M.
author_facet Skakic, Ivana
Francis, Jasmine E.
Dekiwadia, Chaitali
Aibinu, Ibukun
Huq, Mohsina
Taki, Aya C.
Walduck, Anna
Smooker, Peter M.
author_sort Skakic, Ivana
collection PubMed
description Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS) silica templates giving rise to nanocapsules of average diameter 47 nm. Both were shown to be highly monodispersed and relatively homogenous in structure. Various combinations of the nanocapsules in formulation were assessed as vaccines in a mouse model of H. pylori infection. Immune responses were evaluated and protective efficacy assessed. It was demonstrated that vaccination of mice with the larger nanocapsules combined with an adjuvant was able to significantly reduce colonisation.
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spelling pubmed-106742752023-10-27 An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection Skakic, Ivana Francis, Jasmine E. Dekiwadia, Chaitali Aibinu, Ibukun Huq, Mohsina Taki, Aya C. Walduck, Anna Smooker, Peter M. Vaccines (Basel) Article Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS) silica templates giving rise to nanocapsules of average diameter 47 nm. Both were shown to be highly monodispersed and relatively homogenous in structure. Various combinations of the nanocapsules in formulation were assessed as vaccines in a mouse model of H. pylori infection. Immune responses were evaluated and protective efficacy assessed. It was demonstrated that vaccination of mice with the larger nanocapsules combined with an adjuvant was able to significantly reduce colonisation. MDPI 2023-10-27 /pmc/articles/PMC10674275/ /pubmed/38005984 http://dx.doi.org/10.3390/vaccines11111652 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skakic, Ivana
Francis, Jasmine E.
Dekiwadia, Chaitali
Aibinu, Ibukun
Huq, Mohsina
Taki, Aya C.
Walduck, Anna
Smooker, Peter M.
An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title_full An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title_fullStr An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title_full_unstemmed An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title_short An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
title_sort evaluation of urease a subunit nanocapsules as a vaccine in a mouse model of helicobacter pylori infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674275/
https://www.ncbi.nlm.nih.gov/pubmed/38005984
http://dx.doi.org/10.3390/vaccines11111652
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