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An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection
Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS)...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674275/ https://www.ncbi.nlm.nih.gov/pubmed/38005984 http://dx.doi.org/10.3390/vaccines11111652 |
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author | Skakic, Ivana Francis, Jasmine E. Dekiwadia, Chaitali Aibinu, Ibukun Huq, Mohsina Taki, Aya C. Walduck, Anna Smooker, Peter M. |
author_facet | Skakic, Ivana Francis, Jasmine E. Dekiwadia, Chaitali Aibinu, Ibukun Huq, Mohsina Taki, Aya C. Walduck, Anna Smooker, Peter M. |
author_sort | Skakic, Ivana |
collection | PubMed |
description | Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS) silica templates giving rise to nanocapsules of average diameter 47 nm. Both were shown to be highly monodispersed and relatively homogenous in structure. Various combinations of the nanocapsules in formulation were assessed as vaccines in a mouse model of H. pylori infection. Immune responses were evaluated and protective efficacy assessed. It was demonstrated that vaccination of mice with the larger nanocapsules combined with an adjuvant was able to significantly reduce colonisation. |
format | Online Article Text |
id | pubmed-10674275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106742752023-10-27 An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection Skakic, Ivana Francis, Jasmine E. Dekiwadia, Chaitali Aibinu, Ibukun Huq, Mohsina Taki, Aya C. Walduck, Anna Smooker, Peter M. Vaccines (Basel) Article Using removable silica templates, protein nanocapsules comprising the A subunit of Helicobacter pylori urease (UreA) were synthesised. The templates were of two sizes, with solid core mesoporous shell (SC/MS) silica templates giving rise to nanocapsules of average diameter 510 nm and mesoporous (MS) silica templates giving rise to nanocapsules of average diameter 47 nm. Both were shown to be highly monodispersed and relatively homogenous in structure. Various combinations of the nanocapsules in formulation were assessed as vaccines in a mouse model of H. pylori infection. Immune responses were evaluated and protective efficacy assessed. It was demonstrated that vaccination of mice with the larger nanocapsules combined with an adjuvant was able to significantly reduce colonisation. MDPI 2023-10-27 /pmc/articles/PMC10674275/ /pubmed/38005984 http://dx.doi.org/10.3390/vaccines11111652 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Skakic, Ivana Francis, Jasmine E. Dekiwadia, Chaitali Aibinu, Ibukun Huq, Mohsina Taki, Aya C. Walduck, Anna Smooker, Peter M. An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title | An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title_full | An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title_fullStr | An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title_full_unstemmed | An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title_short | An Evaluation of Urease A Subunit Nanocapsules as a Vaccine in a Mouse Model of Helicobacter pylori Infection |
title_sort | evaluation of urease a subunit nanocapsules as a vaccine in a mouse model of helicobacter pylori infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674275/ https://www.ncbi.nlm.nih.gov/pubmed/38005984 http://dx.doi.org/10.3390/vaccines11111652 |
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