Cargando…
Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination
Effective disinfection procedures in healthcare facilities are essential to prevent transmission. Chemical disinfectants, hydrogen peroxide vapour (HPV) systems and ultraviolet (UV) light are commonly used methods. An emerging method, violet-blue light at 405 nm, has shown promise for surface disinf...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674356/ https://www.ncbi.nlm.nih.gov/pubmed/38003802 http://dx.doi.org/10.3390/pathogens12111338 |
_version_ | 1785140808693841920 |
---|---|
author | Amodeo, Davide Manzi, Pietro De Palma, Isa Puccio, Alessandro Nante, Nicola Barcaccia, Mariella Marini, Daniele Pietrella, Donatella |
author_facet | Amodeo, Davide Manzi, Pietro De Palma, Isa Puccio, Alessandro Nante, Nicola Barcaccia, Mariella Marini, Daniele Pietrella, Donatella |
author_sort | Amodeo, Davide |
collection | PubMed |
description | Effective disinfection procedures in healthcare facilities are essential to prevent transmission. Chemical disinfectants, hydrogen peroxide vapour (HPV) systems and ultraviolet (UV) light are commonly used methods. An emerging method, violet-blue light at 405 nm, has shown promise for surface disinfection. Its antimicrobial properties are based on producing reactive oxygen species (ROS) that lead to the inactivation of pathogens. Studies have shown significant efficacy in reducing bacterial levels on surfaces and in the air, reducing nosocomial infections. The aim of this study was to evaluate the antimicrobial effectiveness of violet-blue (405 nm) LED lamps on high-contact surfaces in a hospital infection-control laboratory. High-contact surfaces were sampled before and after 7 days of exposure to violet-blue light. In addition, the effect of violet-blue light on MRSA-contaminated surfaces was investigated. Exposure to violet-blue light significantly reduced the number of bacteria, yeasts and moulds on the sampled surfaces. The incubator handle showed a low microbial load and no growth after irradiation. The worktable and sink showed an inconsistent reduction due to shaded areas. In the second experiment, violet-blue light significantly reduced the microbial load of MRSA on surfaces, with a greater reduction on steel surfaces than on plastic surfaces. Violet-blue light at 405 nm has proven to be an effective tool for pathogen inactivation in healthcare settings Violet-blue light shows promise as an additional and integrated tool to reduce microbial contamination in hospital environments but must be used in combination with standard cleaning practices and infection control protocols. Further research is needed to optimise the violet-blue, 405 nm disinfection method. |
format | Online Article Text |
id | pubmed-10674356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106743562023-11-10 Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination Amodeo, Davide Manzi, Pietro De Palma, Isa Puccio, Alessandro Nante, Nicola Barcaccia, Mariella Marini, Daniele Pietrella, Donatella Pathogens Article Effective disinfection procedures in healthcare facilities are essential to prevent transmission. Chemical disinfectants, hydrogen peroxide vapour (HPV) systems and ultraviolet (UV) light are commonly used methods. An emerging method, violet-blue light at 405 nm, has shown promise for surface disinfection. Its antimicrobial properties are based on producing reactive oxygen species (ROS) that lead to the inactivation of pathogens. Studies have shown significant efficacy in reducing bacterial levels on surfaces and in the air, reducing nosocomial infections. The aim of this study was to evaluate the antimicrobial effectiveness of violet-blue (405 nm) LED lamps on high-contact surfaces in a hospital infection-control laboratory. High-contact surfaces were sampled before and after 7 days of exposure to violet-blue light. In addition, the effect of violet-blue light on MRSA-contaminated surfaces was investigated. Exposure to violet-blue light significantly reduced the number of bacteria, yeasts and moulds on the sampled surfaces. The incubator handle showed a low microbial load and no growth after irradiation. The worktable and sink showed an inconsistent reduction due to shaded areas. In the second experiment, violet-blue light significantly reduced the microbial load of MRSA on surfaces, with a greater reduction on steel surfaces than on plastic surfaces. Violet-blue light at 405 nm has proven to be an effective tool for pathogen inactivation in healthcare settings Violet-blue light shows promise as an additional and integrated tool to reduce microbial contamination in hospital environments but must be used in combination with standard cleaning practices and infection control protocols. Further research is needed to optimise the violet-blue, 405 nm disinfection method. MDPI 2023-11-10 /pmc/articles/PMC10674356/ /pubmed/38003802 http://dx.doi.org/10.3390/pathogens12111338 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Amodeo, Davide Manzi, Pietro De Palma, Isa Puccio, Alessandro Nante, Nicola Barcaccia, Mariella Marini, Daniele Pietrella, Donatella Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title | Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title_full | Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title_fullStr | Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title_full_unstemmed | Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title_short | Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination |
title_sort | efficacy of violet-blue (405 nm) led lamps for disinfection of high-environmental-contact surfaces in healthcare facilities: leading to the inactivation of microorganisms and reduction of mrsa contamination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674356/ https://www.ncbi.nlm.nih.gov/pubmed/38003802 http://dx.doi.org/10.3390/pathogens12111338 |
work_keys_str_mv | AT amodeodavide efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT manzipietro efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT depalmaisa efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT puccioalessandro efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT nantenicola efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT barcacciamariella efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT marinidaniele efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination AT pietrelladonatella efficacyofvioletblue405nmledlampsfordisinfectionofhighenvironmentalcontactsurfacesinhealthcarefacilitiesleadingtotheinactivationofmicroorganismsandreductionofmrsacontamination |