Cargando…
Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes
Skeletal muscle is the key tissue for maintaining protein and glucose homeostasis, having a profound impact on the development of diabetes. Diabetes causes deleterious changes in terms of loss of muscle mass, which will contribute to reduced glucose uptake and therefore progression of the disease. N...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674618/ https://www.ncbi.nlm.nih.gov/pubmed/38004100 http://dx.doi.org/10.3390/nu15224706 |
_version_ | 1785140870481182720 |
---|---|
author | Manzano, Manuel Girón, María D. Salto, Rafael Burgio, Chiara Reinoso, Antonio Cabrera, Elena Rueda, Ricardo López-Pedrosa, Jose M. |
author_facet | Manzano, Manuel Girón, María D. Salto, Rafael Burgio, Chiara Reinoso, Antonio Cabrera, Elena Rueda, Ricardo López-Pedrosa, Jose M. |
author_sort | Manzano, Manuel |
collection | PubMed |
description | Skeletal muscle is the key tissue for maintaining protein and glucose homeostasis, having a profound impact on the development of diabetes. Diabetes causes deleterious changes in terms of loss of muscle mass, which will contribute to reduced glucose uptake and therefore progression of the disease. Nutritional approaches in diabetes have been directed to increase muscle glucose uptake, and improving protein turnover has been at least partially an oversight. In muscle, β-hydroxy β-methyl butyrate (HMB) promotes net protein synthesis, while arginine and lysine increase glucose uptake, albeit their effects on promoting protein synthesis are limited. This study evaluates if the combination of HMB, lysine, and arginine could prevent the loss of muscle mass and function, reducing the progression of diabetes. Therefore, the combination of these ingredients was tested in vitro and in vivo. In muscle cell cultures, the supplementation enhances glucose uptake and net protein synthesis due to an increase in the amount of GLUT4 transporter and stimulation of the insulin-dependent signaling pathway involving IRS-1 and Akt. In vivo, using a rat model of diabetes, the supplementation increases lean body mass and insulin sensitivity and decreases blood glucose and serum glycosylated hemoglobin. In treated animals, an increase in GLUT4, creatine kinase, and Akt phosphorylation was detected, demonstrating the synergic effects of the three ingredients. Our findings showed that nutritional formulations based on the combination of HMB, lysine, and arginine are effective, not only to control blood glucose levels but also to prevent skeletal muscle atrophy associated with the progression of diabetes. |
format | Online Article Text |
id | pubmed-10674618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106746182023-11-07 Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes Manzano, Manuel Girón, María D. Salto, Rafael Burgio, Chiara Reinoso, Antonio Cabrera, Elena Rueda, Ricardo López-Pedrosa, Jose M. Nutrients Article Skeletal muscle is the key tissue for maintaining protein and glucose homeostasis, having a profound impact on the development of diabetes. Diabetes causes deleterious changes in terms of loss of muscle mass, which will contribute to reduced glucose uptake and therefore progression of the disease. Nutritional approaches in diabetes have been directed to increase muscle glucose uptake, and improving protein turnover has been at least partially an oversight. In muscle, β-hydroxy β-methyl butyrate (HMB) promotes net protein synthesis, while arginine and lysine increase glucose uptake, albeit their effects on promoting protein synthesis are limited. This study evaluates if the combination of HMB, lysine, and arginine could prevent the loss of muscle mass and function, reducing the progression of diabetes. Therefore, the combination of these ingredients was tested in vitro and in vivo. In muscle cell cultures, the supplementation enhances glucose uptake and net protein synthesis due to an increase in the amount of GLUT4 transporter and stimulation of the insulin-dependent signaling pathway involving IRS-1 and Akt. In vivo, using a rat model of diabetes, the supplementation increases lean body mass and insulin sensitivity and decreases blood glucose and serum glycosylated hemoglobin. In treated animals, an increase in GLUT4, creatine kinase, and Akt phosphorylation was detected, demonstrating the synergic effects of the three ingredients. Our findings showed that nutritional formulations based on the combination of HMB, lysine, and arginine are effective, not only to control blood glucose levels but also to prevent skeletal muscle atrophy associated with the progression of diabetes. MDPI 2023-11-07 /pmc/articles/PMC10674618/ /pubmed/38004100 http://dx.doi.org/10.3390/nu15224706 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Manzano, Manuel Girón, María D. Salto, Rafael Burgio, Chiara Reinoso, Antonio Cabrera, Elena Rueda, Ricardo López-Pedrosa, Jose M. Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title | Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title_full | Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title_fullStr | Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title_full_unstemmed | Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title_short | Arginine and Lysine Supplementation Potentiates the Beneficial β-Hydroxy ß-Methyl Butyrate (HMB) Effects on Skeletal Muscle in a Rat Model of Diabetes |
title_sort | arginine and lysine supplementation potentiates the beneficial β-hydroxy ß-methyl butyrate (hmb) effects on skeletal muscle in a rat model of diabetes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674618/ https://www.ncbi.nlm.nih.gov/pubmed/38004100 http://dx.doi.org/10.3390/nu15224706 |
work_keys_str_mv | AT manzanomanuel arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT gironmariad arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT saltorafael arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT burgiochiara arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT reinosoantonio arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT cabreraelena arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT ruedaricardo arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes AT lopezpedrosajosem arginineandlysinesupplementationpotentiatesthebeneficialbhydroxyßmethylbutyratehmbeffectsonskeletalmuscleinaratmodelofdiabetes |