Cargando…

Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy

BACKGROUND: Photothermal therapy (PTT) has gained considerable interest as an emerging modality for cancer treatment in recent years. Radiation therapy (RT) has been widely used in the clinic as a traditional treatment method. However, RT and PTT treatments are limited by side effects and penetratio...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Han, Chen, Ji, Qi, Lu He, Lyu, Meng, Quan, Hong, Tan, Zhi Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674778/
https://www.ncbi.nlm.nih.gov/pubmed/38026515
http://dx.doi.org/10.2147/IJN.S422348
_version_ 1785149748600111104
author Tang, Han
Chen, Ji
Qi, Lu He
Lyu, Meng
Quan, Hong
Tan, Zhi Jie
author_facet Tang, Han
Chen, Ji
Qi, Lu He
Lyu, Meng
Quan, Hong
Tan, Zhi Jie
author_sort Tang, Han
collection PubMed
description BACKGROUND: Photothermal therapy (PTT) has gained considerable interest as an emerging modality for cancer treatment in recent years. Radiation therapy (RT) has been widely used in the clinic as a traditional treatment method. However, RT and PTT treatments are limited by side effects and penetration depth, respectively. In addition, hypoxia within the tumor can lead to increased resistance to treatment. METHODS: We synthesized multiple sizes of AuPt by modulating the reaction conditions. The smallest size of AuPt was selected and modified with folic acid (FA) for PTT and RT synergy therapy. Various methods including transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FITR) are used to determine the structure and composition of AuPt-FA (AF). In addition, we researched the photothermal properties of AF with IR cameras and infrared lasers. Flow cytometry, colony formation assays, CCK8, and fluorescent staining for probing the treatment effect in vitro. Also, we explored the targeting of AF by TEM and In Vivo Imaging Systems (IVIS). In vivo experiments, we record changes in tumor volume and weight as well as staining of tumor sections (ROS, Ki67, and hematoxylin and eosin). RESULTS: The AuPt with particle size of 16 nm endows it with remarkably high photothermal conversion efficiency (46.84%) and catalase activity compared to other sizes of AuPt (30 nm and 100 nm). AF alleviates hypoxia in the tumor microenvironment, leading to the production of more reactive oxygen species (ROS) during the treatment. In addition, the therapeutic effect was significantly enhanced by combining RT and PTT, with an apoptosis rate of 81.1% in vitro and an in vivo tumor volume reduction rate of 94.0% in vivo. CONCLUSION: These results demonstrate that AF potentiates the synergistic effect of PTT and RT and has the potential for clinical translation.
format Online
Article
Text
id pubmed-10674778
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-106747782023-11-20 Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy Tang, Han Chen, Ji Qi, Lu He Lyu, Meng Quan, Hong Tan, Zhi Jie Int J Nanomedicine Original Research BACKGROUND: Photothermal therapy (PTT) has gained considerable interest as an emerging modality for cancer treatment in recent years. Radiation therapy (RT) has been widely used in the clinic as a traditional treatment method. However, RT and PTT treatments are limited by side effects and penetration depth, respectively. In addition, hypoxia within the tumor can lead to increased resistance to treatment. METHODS: We synthesized multiple sizes of AuPt by modulating the reaction conditions. The smallest size of AuPt was selected and modified with folic acid (FA) for PTT and RT synergy therapy. Various methods including transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FITR) are used to determine the structure and composition of AuPt-FA (AF). In addition, we researched the photothermal properties of AF with IR cameras and infrared lasers. Flow cytometry, colony formation assays, CCK8, and fluorescent staining for probing the treatment effect in vitro. Also, we explored the targeting of AF by TEM and In Vivo Imaging Systems (IVIS). In vivo experiments, we record changes in tumor volume and weight as well as staining of tumor sections (ROS, Ki67, and hematoxylin and eosin). RESULTS: The AuPt with particle size of 16 nm endows it with remarkably high photothermal conversion efficiency (46.84%) and catalase activity compared to other sizes of AuPt (30 nm and 100 nm). AF alleviates hypoxia in the tumor microenvironment, leading to the production of more reactive oxygen species (ROS) during the treatment. In addition, the therapeutic effect was significantly enhanced by combining RT and PTT, with an apoptosis rate of 81.1% in vitro and an in vivo tumor volume reduction rate of 94.0% in vivo. CONCLUSION: These results demonstrate that AF potentiates the synergistic effect of PTT and RT and has the potential for clinical translation. Dove 2023-11-20 /pmc/articles/PMC10674778/ /pubmed/38026515 http://dx.doi.org/10.2147/IJN.S422348 Text en © 2023 Tang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Tang, Han
Chen, Ji
Qi, Lu He
Lyu, Meng
Quan, Hong
Tan, Zhi Jie
Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title_full Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title_fullStr Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title_full_unstemmed Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title_short Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy
title_sort multifunctional aupt nanoparticles for synergistic photothermal and radiation therapy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674778/
https://www.ncbi.nlm.nih.gov/pubmed/38026515
http://dx.doi.org/10.2147/IJN.S422348
work_keys_str_mv AT tanghan multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy
AT chenji multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy
AT qiluhe multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy
AT lyumeng multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy
AT quanhong multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy
AT tanzhijie multifunctionalauptnanoparticlesforsynergisticphotothermalandradiationtherapy