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Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves

Small non-coding RNAs, such as microRNAs (miRNA) and tRNA-derived fragments (tRF), are known to be involved in post-transcriptional gene regulation. Research has provided evidence that small RNAs may influence immune development in calves. Bovine leukosis is a disease in cattle caused by Bovine Leuk...

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Autores principales: Goldkamp, Anna K., Lahuis, Ciarra H., Hagen, Darren E., Taxis, Tasia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674961/
https://www.ncbi.nlm.nih.gov/pubmed/38003777
http://dx.doi.org/10.3390/pathogens12111312
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author Goldkamp, Anna K.
Lahuis, Ciarra H.
Hagen, Darren E.
Taxis, Tasia M.
author_facet Goldkamp, Anna K.
Lahuis, Ciarra H.
Hagen, Darren E.
Taxis, Tasia M.
author_sort Goldkamp, Anna K.
collection PubMed
description Small non-coding RNAs, such as microRNAs (miRNA) and tRNA-derived fragments (tRF), are known to be involved in post-transcriptional gene regulation. Research has provided evidence that small RNAs may influence immune development in calves. Bovine leukosis is a disease in cattle caused by Bovine Leukemia Virus (BLV) that leads to increased susceptibility to opportunistic pathogens. No research has addressed the potential influence that a maternal BLV infection may have on gene regulation through the differential expression of miRNAs or tRFs in progeny. Blood samples from 14-day old Holstein calves born to BLV-infected dams were collected. Antibodies for BLV were assessed using ELISA and levels of BLV provirus were assessed using qPCR. Total RNA was extracted from whole blood samples for small RNA sequencing. Five miRNAs (bta-miR-1, bta-miR-206, bta-miR-133a, bta-miR-133b, and bta-miR-2450d) and five tRFs (tRF-36-8JZ8RN58X2NF79E, tRF-20-0PF05B2I, tRF-27-W4R951KHZKK, tRF-22-S3M8309NF, and tRF-26-M87SFR2W9J0) were dysregulated in calves born to BLV-infected dams. The miRNAs appear to be involved in the gene regulation of immunological responses and muscle development. The tRF subtypes and parental tRNA profiles in calves born to infected dams appear to be consistent with previous publications in adult cattle with BLV infection. These findings offer insight into how maternal BLV infection status may impact the development of offspring.
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spelling pubmed-106749612023-11-03 Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves Goldkamp, Anna K. Lahuis, Ciarra H. Hagen, Darren E. Taxis, Tasia M. Pathogens Article Small non-coding RNAs, such as microRNAs (miRNA) and tRNA-derived fragments (tRF), are known to be involved in post-transcriptional gene regulation. Research has provided evidence that small RNAs may influence immune development in calves. Bovine leukosis is a disease in cattle caused by Bovine Leukemia Virus (BLV) that leads to increased susceptibility to opportunistic pathogens. No research has addressed the potential influence that a maternal BLV infection may have on gene regulation through the differential expression of miRNAs or tRFs in progeny. Blood samples from 14-day old Holstein calves born to BLV-infected dams were collected. Antibodies for BLV were assessed using ELISA and levels of BLV provirus were assessed using qPCR. Total RNA was extracted from whole blood samples for small RNA sequencing. Five miRNAs (bta-miR-1, bta-miR-206, bta-miR-133a, bta-miR-133b, and bta-miR-2450d) and five tRFs (tRF-36-8JZ8RN58X2NF79E, tRF-20-0PF05B2I, tRF-27-W4R951KHZKK, tRF-22-S3M8309NF, and tRF-26-M87SFR2W9J0) were dysregulated in calves born to BLV-infected dams. The miRNAs appear to be involved in the gene regulation of immunological responses and muscle development. The tRF subtypes and parental tRNA profiles in calves born to infected dams appear to be consistent with previous publications in adult cattle with BLV infection. These findings offer insight into how maternal BLV infection status may impact the development of offspring. MDPI 2023-11-03 /pmc/articles/PMC10674961/ /pubmed/38003777 http://dx.doi.org/10.3390/pathogens12111312 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Goldkamp, Anna K.
Lahuis, Ciarra H.
Hagen, Darren E.
Taxis, Tasia M.
Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title_full Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title_fullStr Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title_full_unstemmed Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title_short Influence of Maternal BLV Infection on miRNA and tRF Expression in Calves
title_sort influence of maternal blv infection on mirna and trf expression in calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10674961/
https://www.ncbi.nlm.nih.gov/pubmed/38003777
http://dx.doi.org/10.3390/pathogens12111312
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