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Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors

Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by...

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Autores principales: Eggers, Maren, Schwebke, Ingeborg, Blümel, Johannes, Brandt, Franziska, Fickenscher, Helmut, Gebel, Jürgen, Hübner, Nils, Müller, Janis A., Rabenau, Holger F., Rapp, Ingrid, Reiche, Sven, Steinmann, Eike, Steinmann, Jochen, Zwicker, Paula, Suchomel, Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675031/
https://www.ncbi.nlm.nih.gov/pubmed/38005856
http://dx.doi.org/10.3390/v15112179
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author Eggers, Maren
Schwebke, Ingeborg
Blümel, Johannes
Brandt, Franziska
Fickenscher, Helmut
Gebel, Jürgen
Hübner, Nils
Müller, Janis A.
Rabenau, Holger F.
Rapp, Ingrid
Reiche, Sven
Steinmann, Eike
Steinmann, Jochen
Zwicker, Paula
Suchomel, Miranda
author_facet Eggers, Maren
Schwebke, Ingeborg
Blümel, Johannes
Brandt, Franziska
Fickenscher, Helmut
Gebel, Jürgen
Hübner, Nils
Müller, Janis A.
Rabenau, Holger F.
Rapp, Ingrid
Reiche, Sven
Steinmann, Eike
Steinmann, Jochen
Zwicker, Paula
Suchomel, Miranda
author_sort Eggers, Maren
collection PubMed
description Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by their activity against test organisms (i.e., viruses, bacteria, and/or yeasts). This activity is usually determined using a suspension test in which the test organism is incubated with the respective disinfectant in solution to assess its bactericidal, yeasticidal, or virucidal activity. In addition, carrier methods that more closely reflect real-world applications have been developed, in which microorganisms are applied to the surface of a carrier (e.g., stainless steel frosted glass, or polyvinyl chloride (PVC)) and then dried. However, to date, no standardized methods have become available for addressing genetically modified vectors or disinfection-resistant oncolytic viruses such as the H1-parvovirus. Particularly, such non-enveloped viruses, which are highly resistant to disinfectants, are not taken into account in European standards. This article proposes a new activity claim known as “virucidal activity PLUS”, summarizes the available methods for evaluating the virucidal activity of chemical disinfectants against genetically modified organisms (GMOs) using current European standards, including the activity against highly resistant parvoviridae such as the adeno-associated virus (AAV), and provides guidance on the selection of disinfectants for pharmaceutical manufacturers, laboratories, and clinical users.
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spelling pubmed-106750312023-10-30 Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors Eggers, Maren Schwebke, Ingeborg Blümel, Johannes Brandt, Franziska Fickenscher, Helmut Gebel, Jürgen Hübner, Nils Müller, Janis A. Rabenau, Holger F. Rapp, Ingrid Reiche, Sven Steinmann, Eike Steinmann, Jochen Zwicker, Paula Suchomel, Miranda Viruses Review Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by their activity against test organisms (i.e., viruses, bacteria, and/or yeasts). This activity is usually determined using a suspension test in which the test organism is incubated with the respective disinfectant in solution to assess its bactericidal, yeasticidal, or virucidal activity. In addition, carrier methods that more closely reflect real-world applications have been developed, in which microorganisms are applied to the surface of a carrier (e.g., stainless steel frosted glass, or polyvinyl chloride (PVC)) and then dried. However, to date, no standardized methods have become available for addressing genetically modified vectors or disinfection-resistant oncolytic viruses such as the H1-parvovirus. Particularly, such non-enveloped viruses, which are highly resistant to disinfectants, are not taken into account in European standards. This article proposes a new activity claim known as “virucidal activity PLUS”, summarizes the available methods for evaluating the virucidal activity of chemical disinfectants against genetically modified organisms (GMOs) using current European standards, including the activity against highly resistant parvoviridae such as the adeno-associated virus (AAV), and provides guidance on the selection of disinfectants for pharmaceutical manufacturers, laboratories, and clinical users. MDPI 2023-10-30 /pmc/articles/PMC10675031/ /pubmed/38005856 http://dx.doi.org/10.3390/v15112179 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Eggers, Maren
Schwebke, Ingeborg
Blümel, Johannes
Brandt, Franziska
Fickenscher, Helmut
Gebel, Jürgen
Hübner, Nils
Müller, Janis A.
Rabenau, Holger F.
Rapp, Ingrid
Reiche, Sven
Steinmann, Eike
Steinmann, Jochen
Zwicker, Paula
Suchomel, Miranda
Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title_full Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title_fullStr Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title_full_unstemmed Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title_short Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
title_sort suitable disinfectants with proven efficacy for genetically modified viruses and viral vectors
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675031/
https://www.ncbi.nlm.nih.gov/pubmed/38005856
http://dx.doi.org/10.3390/v15112179
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