Cargando…
Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors
Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675031/ https://www.ncbi.nlm.nih.gov/pubmed/38005856 http://dx.doi.org/10.3390/v15112179 |
_version_ | 1785140968286060544 |
---|---|
author | Eggers, Maren Schwebke, Ingeborg Blümel, Johannes Brandt, Franziska Fickenscher, Helmut Gebel, Jürgen Hübner, Nils Müller, Janis A. Rabenau, Holger F. Rapp, Ingrid Reiche, Sven Steinmann, Eike Steinmann, Jochen Zwicker, Paula Suchomel, Miranda |
author_facet | Eggers, Maren Schwebke, Ingeborg Blümel, Johannes Brandt, Franziska Fickenscher, Helmut Gebel, Jürgen Hübner, Nils Müller, Janis A. Rabenau, Holger F. Rapp, Ingrid Reiche, Sven Steinmann, Eike Steinmann, Jochen Zwicker, Paula Suchomel, Miranda |
author_sort | Eggers, Maren |
collection | PubMed |
description | Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by their activity against test organisms (i.e., viruses, bacteria, and/or yeasts). This activity is usually determined using a suspension test in which the test organism is incubated with the respective disinfectant in solution to assess its bactericidal, yeasticidal, or virucidal activity. In addition, carrier methods that more closely reflect real-world applications have been developed, in which microorganisms are applied to the surface of a carrier (e.g., stainless steel frosted glass, or polyvinyl chloride (PVC)) and then dried. However, to date, no standardized methods have become available for addressing genetically modified vectors or disinfection-resistant oncolytic viruses such as the H1-parvovirus. Particularly, such non-enveloped viruses, which are highly resistant to disinfectants, are not taken into account in European standards. This article proposes a new activity claim known as “virucidal activity PLUS”, summarizes the available methods for evaluating the virucidal activity of chemical disinfectants against genetically modified organisms (GMOs) using current European standards, including the activity against highly resistant parvoviridae such as the adeno-associated virus (AAV), and provides guidance on the selection of disinfectants for pharmaceutical manufacturers, laboratories, and clinical users. |
format | Online Article Text |
id | pubmed-10675031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106750312023-10-30 Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors Eggers, Maren Schwebke, Ingeborg Blümel, Johannes Brandt, Franziska Fickenscher, Helmut Gebel, Jürgen Hübner, Nils Müller, Janis A. Rabenau, Holger F. Rapp, Ingrid Reiche, Sven Steinmann, Eike Steinmann, Jochen Zwicker, Paula Suchomel, Miranda Viruses Review Viral disinfection is important for medical facilities, the food industry, and the veterinary field, especially in terms of controlling virus outbreaks. Therefore, standardized methods and activity levels are available for these areas. Usually, disinfectants used in these areas are characterized by their activity against test organisms (i.e., viruses, bacteria, and/or yeasts). This activity is usually determined using a suspension test in which the test organism is incubated with the respective disinfectant in solution to assess its bactericidal, yeasticidal, or virucidal activity. In addition, carrier methods that more closely reflect real-world applications have been developed, in which microorganisms are applied to the surface of a carrier (e.g., stainless steel frosted glass, or polyvinyl chloride (PVC)) and then dried. However, to date, no standardized methods have become available for addressing genetically modified vectors or disinfection-resistant oncolytic viruses such as the H1-parvovirus. Particularly, such non-enveloped viruses, which are highly resistant to disinfectants, are not taken into account in European standards. This article proposes a new activity claim known as “virucidal activity PLUS”, summarizes the available methods for evaluating the virucidal activity of chemical disinfectants against genetically modified organisms (GMOs) using current European standards, including the activity against highly resistant parvoviridae such as the adeno-associated virus (AAV), and provides guidance on the selection of disinfectants for pharmaceutical manufacturers, laboratories, and clinical users. MDPI 2023-10-30 /pmc/articles/PMC10675031/ /pubmed/38005856 http://dx.doi.org/10.3390/v15112179 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Eggers, Maren Schwebke, Ingeborg Blümel, Johannes Brandt, Franziska Fickenscher, Helmut Gebel, Jürgen Hübner, Nils Müller, Janis A. Rabenau, Holger F. Rapp, Ingrid Reiche, Sven Steinmann, Eike Steinmann, Jochen Zwicker, Paula Suchomel, Miranda Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title | Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title_full | Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title_fullStr | Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title_full_unstemmed | Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title_short | Suitable Disinfectants with Proven Efficacy for Genetically Modified Viruses and Viral Vectors |
title_sort | suitable disinfectants with proven efficacy for genetically modified viruses and viral vectors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675031/ https://www.ncbi.nlm.nih.gov/pubmed/38005856 http://dx.doi.org/10.3390/v15112179 |
work_keys_str_mv | AT eggersmaren suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT schwebkeingeborg suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT blumeljohannes suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT brandtfranziska suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT fickenscherhelmut suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT gebeljurgen suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT hubnernils suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT mullerjanisa suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT rabenauholgerf suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT rappingrid suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT reichesven suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT steinmanneike suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT steinmannjochen suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT zwickerpaula suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors AT suchomelmiranda suitabledisinfectantswithprovenefficacyforgeneticallymodifiedvirusesandviralvectors |