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Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics

Although regenerative medicine necessitates advanced three-dimensional (3D) scaffolds for organ and tissue applications, creating intricate structures across scales, from nano- to meso-like biological tissues, remains a challenge. Electrospinning of nanofibers offers promise due to its capacity to c...

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Detalles Bibliográficos
Autores principales: Raje, Komal, Ohashi, Keisuke, Fujita, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675084/
https://www.ncbi.nlm.nih.gov/pubmed/37999268
http://dx.doi.org/10.3390/nano13222913
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author Raje, Komal
Ohashi, Keisuke
Fujita, Satoshi
author_facet Raje, Komal
Ohashi, Keisuke
Fujita, Satoshi
author_sort Raje, Komal
collection PubMed
description Although regenerative medicine necessitates advanced three-dimensional (3D) scaffolds for organ and tissue applications, creating intricate structures across scales, from nano- to meso-like biological tissues, remains a challenge. Electrospinning of nanofibers offers promise due to its capacity to craft not only the dimensions and surfaces of individual fibers but also intricate attributes, such as anisotropy and porosity, across various materials. In this study, we used a 3D printer to design a mold with polylactic acid for gel modeling. This gel template, which was mounted on a metal wire, facilitated microfiber electrospinning. After spinning, these structures were treated with EDTA to remove the template and were then cleansed and dried, resulting in 3D microfibrous (3DMF) structures, with average fiber diameters of approximately 1 µm on the outer and inner surfaces. Notably, these structures matched their intended design dimensions without distortion or shrinkage, demonstrating the adaptability of this method for various template sizes. The cylindrical structures showed high elasticity and stretchability with an elastic modulus of 6.23 MPa. Furthermore, our method successfully mimicked complex biological tissue structures, such as the inner architecture of the voice box and the hollow partitioned structure of the heart’s tricuspid valve. Achieving specific intricate shapes required multiple spinning sessions and subsequent assemblies. In essence, our approach holds potential for crafting artificial organs and forming the foundational materials for cell culture scaffolds, addressing the challenges of crafting intricate multiscale structures.
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spelling pubmed-106750842023-11-08 Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics Raje, Komal Ohashi, Keisuke Fujita, Satoshi Nanomaterials (Basel) Article Although regenerative medicine necessitates advanced three-dimensional (3D) scaffolds for organ and tissue applications, creating intricate structures across scales, from nano- to meso-like biological tissues, remains a challenge. Electrospinning of nanofibers offers promise due to its capacity to craft not only the dimensions and surfaces of individual fibers but also intricate attributes, such as anisotropy and porosity, across various materials. In this study, we used a 3D printer to design a mold with polylactic acid for gel modeling. This gel template, which was mounted on a metal wire, facilitated microfiber electrospinning. After spinning, these structures were treated with EDTA to remove the template and were then cleansed and dried, resulting in 3D microfibrous (3DMF) structures, with average fiber diameters of approximately 1 µm on the outer and inner surfaces. Notably, these structures matched their intended design dimensions without distortion or shrinkage, demonstrating the adaptability of this method for various template sizes. The cylindrical structures showed high elasticity and stretchability with an elastic modulus of 6.23 MPa. Furthermore, our method successfully mimicked complex biological tissue structures, such as the inner architecture of the voice box and the hollow partitioned structure of the heart’s tricuspid valve. Achieving specific intricate shapes required multiple spinning sessions and subsequent assemblies. In essence, our approach holds potential for crafting artificial organs and forming the foundational materials for cell culture scaffolds, addressing the challenges of crafting intricate multiscale structures. MDPI 2023-11-08 /pmc/articles/PMC10675084/ /pubmed/37999268 http://dx.doi.org/10.3390/nano13222913 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raje, Komal
Ohashi, Keisuke
Fujita, Satoshi
Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title_full Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title_fullStr Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title_full_unstemmed Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title_short Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics
title_sort three-dimensional printer-assisted electrospinning for fabricating intricate biological tissue mimics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675084/
https://www.ncbi.nlm.nih.gov/pubmed/37999268
http://dx.doi.org/10.3390/nano13222913
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