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miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice

Plant miRNAs are a class of noncoding RNA with a length of 21–24 nt that play an important role in plant responses to biotic and abiotic stresses. Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases in rice. Our previous work showed that...

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Autores principales: Zhu, Xiumei, Kuang, Yongjie, Chen, Yutong, Shi, Jia, Cao, Yaqian, Hu, Jixiang, Yu, Chao, Yang, Fenghuan, Tian, Fang, Chen, Huamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675396/
https://www.ncbi.nlm.nih.gov/pubmed/38005712
http://dx.doi.org/10.3390/plants12223815
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author Zhu, Xiumei
Kuang, Yongjie
Chen, Yutong
Shi, Jia
Cao, Yaqian
Hu, Jixiang
Yu, Chao
Yang, Fenghuan
Tian, Fang
Chen, Huamin
author_facet Zhu, Xiumei
Kuang, Yongjie
Chen, Yutong
Shi, Jia
Cao, Yaqian
Hu, Jixiang
Yu, Chao
Yang, Fenghuan
Tian, Fang
Chen, Huamin
author_sort Zhu, Xiumei
collection PubMed
description Plant miRNAs are a class of noncoding RNA with a length of 21–24 nt that play an important role in plant responses to biotic and abiotic stresses. Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases in rice. Our previous work showed that osa-miR2118b/n was induced by Xoo infection. However, the biological function of miR2118 has not yet been characterized in experiments. Herein, we constructed MIR2118b OE, as well as single and double mutants of MIR2118b/n using CRISPR/Cas9. Further results showed that osa-MIR2118b OE plants exhibited longer lesion lengths than the wild type after Xoo inoculation, while MIR2118 CRISPR plants exhibited shorter lesion lengths than the wild type after Xoo inoculation. Co-transformation experiments in rice protoplasts indicated that osa-miR2118 negatively regulated the transcripts of three nucleotide-binding sites and leucine-rich repeat (NLR) genes (LOC_Os08g42700.1, LOC_Os01g05600.1, and LOC_Os12g37290.1) which are predicted target genes of miR2118, but not the mutated NLR genes with a 3 bp insertion at the center of the binding sites. The transcriptional level of the three NLR genes was reversed relative to osa-miR2118 in the MIR2118b OE and MIR2118b CRISPR plants. The above results demonstrate that osa-miR2118b/n negatively regulates the resistance to bacterial blight through negatively regulating several NLR genes.
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spelling pubmed-106753962023-11-09 miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice Zhu, Xiumei Kuang, Yongjie Chen, Yutong Shi, Jia Cao, Yaqian Hu, Jixiang Yu, Chao Yang, Fenghuan Tian, Fang Chen, Huamin Plants (Basel) Brief Report Plant miRNAs are a class of noncoding RNA with a length of 21–24 nt that play an important role in plant responses to biotic and abiotic stresses. Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases in rice. Our previous work showed that osa-miR2118b/n was induced by Xoo infection. However, the biological function of miR2118 has not yet been characterized in experiments. Herein, we constructed MIR2118b OE, as well as single and double mutants of MIR2118b/n using CRISPR/Cas9. Further results showed that osa-MIR2118b OE plants exhibited longer lesion lengths than the wild type after Xoo inoculation, while MIR2118 CRISPR plants exhibited shorter lesion lengths than the wild type after Xoo inoculation. Co-transformation experiments in rice protoplasts indicated that osa-miR2118 negatively regulated the transcripts of three nucleotide-binding sites and leucine-rich repeat (NLR) genes (LOC_Os08g42700.1, LOC_Os01g05600.1, and LOC_Os12g37290.1) which are predicted target genes of miR2118, but not the mutated NLR genes with a 3 bp insertion at the center of the binding sites. The transcriptional level of the three NLR genes was reversed relative to osa-miR2118 in the MIR2118b OE and MIR2118b CRISPR plants. The above results demonstrate that osa-miR2118b/n negatively regulates the resistance to bacterial blight through negatively regulating several NLR genes. MDPI 2023-11-09 /pmc/articles/PMC10675396/ /pubmed/38005712 http://dx.doi.org/10.3390/plants12223815 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Zhu, Xiumei
Kuang, Yongjie
Chen, Yutong
Shi, Jia
Cao, Yaqian
Hu, Jixiang
Yu, Chao
Yang, Fenghuan
Tian, Fang
Chen, Huamin
miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title_full miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title_fullStr miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title_full_unstemmed miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title_short miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice
title_sort mir2118 negatively regulates bacterial blight resistance through targeting several disease resistance genes in rice
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675396/
https://www.ncbi.nlm.nih.gov/pubmed/38005712
http://dx.doi.org/10.3390/plants12223815
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