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Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia

Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and evaluate...

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Autores principales: Choi, Minsol, Yoon, Jiwon, Yang, So Hwi, Kim, Jae Kwang, Park, Sang Un
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675400/
https://www.ncbi.nlm.nih.gov/pubmed/38005737
http://dx.doi.org/10.3390/plants12223840
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author Choi, Minsol
Yoon, Jiwon
Yang, So Hwi
Kim, Jae Kwang
Park, Sang Un
author_facet Choi, Minsol
Yoon, Jiwon
Yang, So Hwi
Kim, Jae Kwang
Park, Sang Un
author_sort Choi, Minsol
collection PubMed
description Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and evaluated its antioxidant activities. Rosmarinic acid and five phenylpropanoids were detected using high-performance liquid chromatography. The hairy roots grown in 1/2 SH medium with 1 mg/L NAA had a high level of rosmarinic acid content. Hairy roots cultured in 1 mg/L NAA had the highest total content of five phenylpropanoids. Compared to wild-type roots grown in the field, hairy roots (NAA 1) expressed similar levels of rosmarinic acid but significantly enhanced phenylpropanoid accumulation. Furthermore, the total phenolic content and total flavonoid content of hairy roots (NAA 1) were 2.22 and 1.73 times higher than those of wild-type roots. In the results of DPPH, ABTS, and reducing power assays, the hairy roots (NAA 1) showed higher free radical scavenging effects and reduction potential than the wild-type roots. These results suggest that S. plebeia hairy roots cultured under optimal conditions, which exhibit enhanced phenolic compound accumulation and antioxidant activity, can potentially be used as sources of antioxidants.
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spelling pubmed-106754002023-11-13 Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia Choi, Minsol Yoon, Jiwon Yang, So Hwi Kim, Jae Kwang Park, Sang Un Plants (Basel) Article Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and evaluated its antioxidant activities. Rosmarinic acid and five phenylpropanoids were detected using high-performance liquid chromatography. The hairy roots grown in 1/2 SH medium with 1 mg/L NAA had a high level of rosmarinic acid content. Hairy roots cultured in 1 mg/L NAA had the highest total content of five phenylpropanoids. Compared to wild-type roots grown in the field, hairy roots (NAA 1) expressed similar levels of rosmarinic acid but significantly enhanced phenylpropanoid accumulation. Furthermore, the total phenolic content and total flavonoid content of hairy roots (NAA 1) were 2.22 and 1.73 times higher than those of wild-type roots. In the results of DPPH, ABTS, and reducing power assays, the hairy roots (NAA 1) showed higher free radical scavenging effects and reduction potential than the wild-type roots. These results suggest that S. plebeia hairy roots cultured under optimal conditions, which exhibit enhanced phenolic compound accumulation and antioxidant activity, can potentially be used as sources of antioxidants. MDPI 2023-11-13 /pmc/articles/PMC10675400/ /pubmed/38005737 http://dx.doi.org/10.3390/plants12223840 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Minsol
Yoon, Jiwon
Yang, So Hwi
Kim, Jae Kwang
Park, Sang Un
Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title_full Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title_fullStr Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title_full_unstemmed Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title_short Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia
title_sort production of phenolic compounds and antioxidant activity in hairy root cultures of salvia plebeia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675400/
https://www.ncbi.nlm.nih.gov/pubmed/38005737
http://dx.doi.org/10.3390/plants12223840
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