Cargando…
Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for in...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675509/ https://www.ncbi.nlm.nih.gov/pubmed/38005719 http://dx.doi.org/10.3390/plants12223821 |
_version_ | 1785141081851035648 |
---|---|
author | Ferreira, Patrick Chahed, Abdessalem Estevinho, Letícia M. Seixas, Natália Costa, Rodrigo Choupina, Altino |
author_facet | Ferreira, Patrick Chahed, Abdessalem Estevinho, Letícia M. Seixas, Natália Costa, Rodrigo Choupina, Altino |
author_sort | Ferreira, Patrick |
collection | PubMed |
description | Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi. |
format | Online Article Text |
id | pubmed-10675509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106755092023-11-10 Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi Ferreira, Patrick Chahed, Abdessalem Estevinho, Letícia M. Seixas, Natália Costa, Rodrigo Choupina, Altino Plants (Basel) Article Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi. MDPI 2023-11-10 /pmc/articles/PMC10675509/ /pubmed/38005719 http://dx.doi.org/10.3390/plants12223821 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferreira, Patrick Chahed, Abdessalem Estevinho, Letícia M. Seixas, Natália Costa, Rodrigo Choupina, Altino Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title | Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title_full | Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title_fullStr | Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title_full_unstemmed | Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title_short | Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi |
title_sort | post-transcriptional gene silencing of glucanase inhibitor protein in phytophthora cinnamomi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675509/ https://www.ncbi.nlm.nih.gov/pubmed/38005719 http://dx.doi.org/10.3390/plants12223821 |
work_keys_str_mv | AT ferreirapatrick posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi AT chahedabdessalem posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi AT estevinholeticiam posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi AT seixasnatalia posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi AT costarodrigo posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi AT choupinaaltino posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi |