Cargando…

Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi

Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for in...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferreira, Patrick, Chahed, Abdessalem, Estevinho, Letícia M., Seixas, Natália, Costa, Rodrigo, Choupina, Altino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675509/
https://www.ncbi.nlm.nih.gov/pubmed/38005719
http://dx.doi.org/10.3390/plants12223821
_version_ 1785141081851035648
author Ferreira, Patrick
Chahed, Abdessalem
Estevinho, Letícia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
author_facet Ferreira, Patrick
Chahed, Abdessalem
Estevinho, Letícia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
author_sort Ferreira, Patrick
collection PubMed
description Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi.
format Online
Article
Text
id pubmed-10675509
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106755092023-11-10 Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi Ferreira, Patrick Chahed, Abdessalem Estevinho, Letícia M. Seixas, Natália Costa, Rodrigo Choupina, Altino Plants (Basel) Article Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Castanea sativa Mill can be attributed to two main species: Phytophthora cinnamomi and Phytophthora cambivora, with the first being the main pathogen responsible for ink disease in Portugal. P. cinnamomi is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut’s economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between Phytophthora species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by P. cinnamomi during infection. The technique of RNA interference (RNAi) was employed to suppress the GIP gene of P. cinnamomi. The resulting transformants, carrying the silenced gene, were used to infect C. sativa, allowing for the assessment of the effects of gene silencing on the plant’s phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing P. cinnamomi. MDPI 2023-11-10 /pmc/articles/PMC10675509/ /pubmed/38005719 http://dx.doi.org/10.3390/plants12223821 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferreira, Patrick
Chahed, Abdessalem
Estevinho, Letícia M.
Seixas, Natália
Costa, Rodrigo
Choupina, Altino
Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title_full Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title_fullStr Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title_full_unstemmed Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title_short Post-Transcriptional Gene Silencing of Glucanase Inhibitor Protein in Phytophthora cinnamomi
title_sort post-transcriptional gene silencing of glucanase inhibitor protein in phytophthora cinnamomi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675509/
https://www.ncbi.nlm.nih.gov/pubmed/38005719
http://dx.doi.org/10.3390/plants12223821
work_keys_str_mv AT ferreirapatrick posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi
AT chahedabdessalem posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi
AT estevinholeticiam posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi
AT seixasnatalia posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi
AT costarodrigo posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi
AT choupinaaltino posttranscriptionalgenesilencingofglucanaseinhibitorproteininphytophthoracinnamomi