Cargando…

Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management

The purpose of the study is to develop a novel peptide for caries management. Gallic-Acid-Polyphemusin-I (GAPI) was synthesised by grafting Polyphemusin I (PI) and gallic acid (GA). Biocompatibility was evaluated using a Cell Counting Kit-8 Assay. Antimicrobial properties were assessed using minimum...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Olivia Lili, Niu, John Yun, Yu, Ollie Yiru, Mei, May Lei, Jakubovics, Nicholas Stephen, Chu, Chun Hung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675526/
https://www.ncbi.nlm.nih.gov/pubmed/38004539
http://dx.doi.org/10.3390/pharmaceutics15112560
_version_ 1785141085780049920
author Zhang, Olivia Lili
Niu, John Yun
Yu, Ollie Yiru
Mei, May Lei
Jakubovics, Nicholas Stephen
Chu, Chun Hung
author_facet Zhang, Olivia Lili
Niu, John Yun
Yu, Ollie Yiru
Mei, May Lei
Jakubovics, Nicholas Stephen
Chu, Chun Hung
author_sort Zhang, Olivia Lili
collection PubMed
description The purpose of the study is to develop a novel peptide for caries management. Gallic-Acid-Polyphemusin-I (GAPI) was synthesised by grafting Polyphemusin I (PI) and gallic acid (GA). Biocompatibility was evaluated using a Cell Counting Kit-8 Assay. Antimicrobial properties were assessed using minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The bacterial and fungal morphology after GAPI treatment was investigated using transmission electron microscopy (TEM). The architecture of a consortium biofilm consisting of Streptococcus mutans, Lacticaseibacillus casei and Candida albicans was evaluated using scanning electron microscopy (SEM) and confocal laser scanning microscopy. The growth kinetics of the biofilm was examined using a propidium monoazide–quantitative polymerase chain reaction. The surface and calcium-to-phosphorus molar ratio of GAPI-treated enamel after pH cycling were examined with SEM and energy-dispersive X-ray spectroscopy. Enamel crystal characteristics were analysed using X-ray diffraction. Lesion depths representing the enamel’s mineral loss were assessed using micro-computed tomography. The MIC of GAPI against S. mutans, L. casei and C. albicans were 40 μM, 40 μM and 20 μM, respectively. GAPI destroyed the biofilm’s three-dimensional structure and inhibited the growth of the biofilm. SEM showed that enamel treated with GAPI had a relatively smooth surface compared to that treated with water. The calcium-to-phosphorus molar ratio of enamel treated with GAPI was higher than that of the control. The lesion depths and mineral loss of the GAPI-treated enamel were less than the control. The crystallinity of the GAPI-treated enamel was higher than the control. This study developed a biocompatible, mineralising and antimicrobial peptide GAPI, which may have potential as an anti-caries agent.
format Online
Article
Text
id pubmed-10675526
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106755262023-10-31 Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management Zhang, Olivia Lili Niu, John Yun Yu, Ollie Yiru Mei, May Lei Jakubovics, Nicholas Stephen Chu, Chun Hung Pharmaceutics Article The purpose of the study is to develop a novel peptide for caries management. Gallic-Acid-Polyphemusin-I (GAPI) was synthesised by grafting Polyphemusin I (PI) and gallic acid (GA). Biocompatibility was evaluated using a Cell Counting Kit-8 Assay. Antimicrobial properties were assessed using minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The bacterial and fungal morphology after GAPI treatment was investigated using transmission electron microscopy (TEM). The architecture of a consortium biofilm consisting of Streptococcus mutans, Lacticaseibacillus casei and Candida albicans was evaluated using scanning electron microscopy (SEM) and confocal laser scanning microscopy. The growth kinetics of the biofilm was examined using a propidium monoazide–quantitative polymerase chain reaction. The surface and calcium-to-phosphorus molar ratio of GAPI-treated enamel after pH cycling were examined with SEM and energy-dispersive X-ray spectroscopy. Enamel crystal characteristics were analysed using X-ray diffraction. Lesion depths representing the enamel’s mineral loss were assessed using micro-computed tomography. The MIC of GAPI against S. mutans, L. casei and C. albicans were 40 μM, 40 μM and 20 μM, respectively. GAPI destroyed the biofilm’s three-dimensional structure and inhibited the growth of the biofilm. SEM showed that enamel treated with GAPI had a relatively smooth surface compared to that treated with water. The calcium-to-phosphorus molar ratio of enamel treated with GAPI was higher than that of the control. The lesion depths and mineral loss of the GAPI-treated enamel were less than the control. The crystallinity of the GAPI-treated enamel was higher than the control. This study developed a biocompatible, mineralising and antimicrobial peptide GAPI, which may have potential as an anti-caries agent. MDPI 2023-10-31 /pmc/articles/PMC10675526/ /pubmed/38004539 http://dx.doi.org/10.3390/pharmaceutics15112560 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Olivia Lili
Niu, John Yun
Yu, Ollie Yiru
Mei, May Lei
Jakubovics, Nicholas Stephen
Chu, Chun Hung
Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title_full Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title_fullStr Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title_full_unstemmed Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title_short Development of a Novel Peptide with Antimicrobial and Mineralising Properties for Caries Management
title_sort development of a novel peptide with antimicrobial and mineralising properties for caries management
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675526/
https://www.ncbi.nlm.nih.gov/pubmed/38004539
http://dx.doi.org/10.3390/pharmaceutics15112560
work_keys_str_mv AT zhangolivialili developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement
AT niujohnyun developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement
AT yuollieyiru developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement
AT meimaylei developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement
AT jakubovicsnicholasstephen developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement
AT chuchunhung developmentofanovelpeptidewithantimicrobialandmineralisingpropertiesforcariesmanagement