Cargando…

OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice

Reactive oxygen species (ROS) play dual roles in plant stress response, but how plants modulate the dual roles of ROS in stress response is still obscure. OsJAB1 (JUN-activation-domain-binding protein 1) encodes the rice CSN5 (COP9 signalsome subunit 5). This study showed that, similar to the Arabid...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jiayi, Zhang, Chuanyu, Li, Hua, Xu, Yuejun, Zhang, Bo, Zheng, Fuyu, Zhao, Beiping, Zhang, Haiwen, Zhao, Hui, Liu, Baohai, Xiao, Minggang, Zhang, Zhijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675544/
https://www.ncbi.nlm.nih.gov/pubmed/38005759
http://dx.doi.org/10.3390/plants12223859
_version_ 1785149828332781568
author Wang, Jiayi
Zhang, Chuanyu
Li, Hua
Xu, Yuejun
Zhang, Bo
Zheng, Fuyu
Zhao, Beiping
Zhang, Haiwen
Zhao, Hui
Liu, Baohai
Xiao, Minggang
Zhang, Zhijin
author_facet Wang, Jiayi
Zhang, Chuanyu
Li, Hua
Xu, Yuejun
Zhang, Bo
Zheng, Fuyu
Zhao, Beiping
Zhang, Haiwen
Zhao, Hui
Liu, Baohai
Xiao, Minggang
Zhang, Zhijin
author_sort Wang, Jiayi
collection PubMed
description Reactive oxygen species (ROS) play dual roles in plant stress response, but how plants modulate the dual roles of ROS in stress response is still obscure. OsJAB1 (JUN-activation-domain-binding protein 1) encodes the rice CSN5 (COP9 signalsome subunit 5). This study showed that, similar to the Arabidopsis homolog gene CSN5B, OsJAB1-overexpressing (driven by a CaMV 35S promoter) plants (OEs) impaired rice salt stress tolerance; in contrast, OsJAB1-inhibited-expression (using RNA-interfering technology) plants (RIs) enhanced rice salt stress tolerance. Differing from CSN5B that negatively regulated ascorbate (Asc) biosynthesis, Asc content increased in OEs and decreased in RIs. ROS analysis showed that RIs clearly increased, but OEs inhibited ROS accumulation at the early stage of salt treatment; in contrast, RIs clearly decreased, but OEs promoted ROS accumulation at the late stage of salt treatment. The qPCR revealed that OEs decreased but RIs enhanced the expressions of ROS-scavenging genes. This indicated that OsJAB1 negatively regulated rice salt stress tolerance by suppressing the expression of ROS-scavenging genes. This study provided new insights into the CSN5 homologous protein named OsJAB1 in rice, which developed different functions during long-term evolution. How OsJAB1 regulates the Asc biosynthesis that coordinates the balance between cell redox signaling and ROS scavenging needs to be investigated in the future.
format Online
Article
Text
id pubmed-10675544
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106755442023-11-15 OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice Wang, Jiayi Zhang, Chuanyu Li, Hua Xu, Yuejun Zhang, Bo Zheng, Fuyu Zhao, Beiping Zhang, Haiwen Zhao, Hui Liu, Baohai Xiao, Minggang Zhang, Zhijin Plants (Basel) Article Reactive oxygen species (ROS) play dual roles in plant stress response, but how plants modulate the dual roles of ROS in stress response is still obscure. OsJAB1 (JUN-activation-domain-binding protein 1) encodes the rice CSN5 (COP9 signalsome subunit 5). This study showed that, similar to the Arabidopsis homolog gene CSN5B, OsJAB1-overexpressing (driven by a CaMV 35S promoter) plants (OEs) impaired rice salt stress tolerance; in contrast, OsJAB1-inhibited-expression (using RNA-interfering technology) plants (RIs) enhanced rice salt stress tolerance. Differing from CSN5B that negatively regulated ascorbate (Asc) biosynthesis, Asc content increased in OEs and decreased in RIs. ROS analysis showed that RIs clearly increased, but OEs inhibited ROS accumulation at the early stage of salt treatment; in contrast, RIs clearly decreased, but OEs promoted ROS accumulation at the late stage of salt treatment. The qPCR revealed that OEs decreased but RIs enhanced the expressions of ROS-scavenging genes. This indicated that OsJAB1 negatively regulated rice salt stress tolerance by suppressing the expression of ROS-scavenging genes. This study provided new insights into the CSN5 homologous protein named OsJAB1 in rice, which developed different functions during long-term evolution. How OsJAB1 regulates the Asc biosynthesis that coordinates the balance between cell redox signaling and ROS scavenging needs to be investigated in the future. MDPI 2023-11-15 /pmc/articles/PMC10675544/ /pubmed/38005759 http://dx.doi.org/10.3390/plants12223859 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jiayi
Zhang, Chuanyu
Li, Hua
Xu, Yuejun
Zhang, Bo
Zheng, Fuyu
Zhao, Beiping
Zhang, Haiwen
Zhao, Hui
Liu, Baohai
Xiao, Minggang
Zhang, Zhijin
OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title_full OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title_fullStr OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title_full_unstemmed OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title_short OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice
title_sort osjab1 positively regulates ascorbate biosynthesis and negatively regulates salt tolerance due to inhibiting early-stage salt-induced ros accumulation in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675544/
https://www.ncbi.nlm.nih.gov/pubmed/38005759
http://dx.doi.org/10.3390/plants12223859
work_keys_str_mv AT wangjiayi osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhangchuanyu osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT lihua osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT xuyuejun osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhangbo osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhengfuyu osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhaobeiping osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhanghaiwen osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhaohui osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT liubaohai osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT xiaominggang osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice
AT zhangzhijin osjab1positivelyregulatesascorbatebiosynthesisandnegativelyregulatessalttoleranceduetoinhibitingearlystagesaltinducedrosaccumulationinrice