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Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides

Amblyomma sculptum is a relevant tick species from a One Health perspective, playing an important role as a vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. In this study, we evaluated the susceptibility of two A. sculptum populations from Goiás state (midwes...

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Autores principales: Cardoso, Ennya Rafaella Neves, Carvalho, Stephani Félix, Dias, Sarah Alves, Santos, Rayane Almeida, Tavares, Mariana Avelar, Neves, Lucianne Cardoso, Paula, Warley Vieira de Freitas, Pádua, Gracielle Teles, de Lima, Nicolas Jalowitzki, Paludo, Raquel Loren dos Reis, Silva, Isabela Santos, Bittencourt, Raphaela Bueno Mendes, dos Santos, Gabriel Cândido, Nascimento, Flavia Giovana de Jesus, de Paula, Luiza Gabriella Ferreira, Dantas-Torres, Filipe, Monteiro, Caio Marcio De Oliveira, Krawczak, Felipe da Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675591/
https://www.ncbi.nlm.nih.gov/pubmed/38003769
http://dx.doi.org/10.3390/pathogens12111304
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author Cardoso, Ennya Rafaella Neves
Carvalho, Stephani Félix
Dias, Sarah Alves
Santos, Rayane Almeida
Tavares, Mariana Avelar
Neves, Lucianne Cardoso
Paula, Warley Vieira de Freitas
Pádua, Gracielle Teles
de Lima, Nicolas Jalowitzki
Paludo, Raquel Loren dos Reis
Silva, Isabela Santos
Bittencourt, Raphaela Bueno Mendes
dos Santos, Gabriel Cândido
Nascimento, Flavia Giovana de Jesus
de Paula, Luiza Gabriella Ferreira
Dantas-Torres, Filipe
Monteiro, Caio Marcio De Oliveira
Krawczak, Felipe da Silva
author_facet Cardoso, Ennya Rafaella Neves
Carvalho, Stephani Félix
Dias, Sarah Alves
Santos, Rayane Almeida
Tavares, Mariana Avelar
Neves, Lucianne Cardoso
Paula, Warley Vieira de Freitas
Pádua, Gracielle Teles
de Lima, Nicolas Jalowitzki
Paludo, Raquel Loren dos Reis
Silva, Isabela Santos
Bittencourt, Raphaela Bueno Mendes
dos Santos, Gabriel Cândido
Nascimento, Flavia Giovana de Jesus
de Paula, Luiza Gabriella Ferreira
Dantas-Torres, Filipe
Monteiro, Caio Marcio De Oliveira
Krawczak, Felipe da Silva
author_sort Cardoso, Ennya Rafaella Neves
collection PubMed
description Amblyomma sculptum is a relevant tick species from a One Health perspective, playing an important role as a vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. In this study, we evaluated the susceptibility of two A. sculptum populations from Goiás state (midwestern Brazil) to different acaricides. The first tick population (GYN strain) originated from an experimental farm, where the ticks are annually exposed to acaricides. The second (PNE strain) was collected in a national park (Emas National Park), where the ticks had not been exposed to acaricides. Immersion tests were conducted with 21-day-old laboratory-reared larvae and nymphs originating from adult ticks collected in the areas mentioned above. The chosen acaricides were two synthetic pyrethroids (cypermethrin and deltamethrin), one organophosphate (chlorfenvinphos), one formamidine (amitraz), and two combinations of pyrethroids and organophosphates (cypermethrin, chlorpyrifos and citronellal; cypermethrin, fenthion and chlorpyrifos). Mortality data were used to determine the lethal concentration (LC) values at which 50%, 90%, and 99% of the ticks died (LC(50), LC(90), and LC(99), respectively), and resistance ratios (RR) were calculated based on the LC values. The RR revealed differences between the acaricide-exposed (GYN) and unexposed (PNE) tick strains. The PNE strain larvae and nymphs were susceptible to all the tested acaricides. The GYN strain larvae were tolerant to cypermethrin, whereas the nymphs were tolerant to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal (2 < RR ≤ 10). The GYN strain nymphs were resistant to amitraz (RR > 10). This is the first report of A. sculptum nymphs with resistance to amitraz and tolerance to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal.
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spelling pubmed-106755912023-10-31 Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides Cardoso, Ennya Rafaella Neves Carvalho, Stephani Félix Dias, Sarah Alves Santos, Rayane Almeida Tavares, Mariana Avelar Neves, Lucianne Cardoso Paula, Warley Vieira de Freitas Pádua, Gracielle Teles de Lima, Nicolas Jalowitzki Paludo, Raquel Loren dos Reis Silva, Isabela Santos Bittencourt, Raphaela Bueno Mendes dos Santos, Gabriel Cândido Nascimento, Flavia Giovana de Jesus de Paula, Luiza Gabriella Ferreira Dantas-Torres, Filipe Monteiro, Caio Marcio De Oliveira Krawczak, Felipe da Silva Pathogens Article Amblyomma sculptum is a relevant tick species from a One Health perspective, playing an important role as a vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. In this study, we evaluated the susceptibility of two A. sculptum populations from Goiás state (midwestern Brazil) to different acaricides. The first tick population (GYN strain) originated from an experimental farm, where the ticks are annually exposed to acaricides. The second (PNE strain) was collected in a national park (Emas National Park), where the ticks had not been exposed to acaricides. Immersion tests were conducted with 21-day-old laboratory-reared larvae and nymphs originating from adult ticks collected in the areas mentioned above. The chosen acaricides were two synthetic pyrethroids (cypermethrin and deltamethrin), one organophosphate (chlorfenvinphos), one formamidine (amitraz), and two combinations of pyrethroids and organophosphates (cypermethrin, chlorpyrifos and citronellal; cypermethrin, fenthion and chlorpyrifos). Mortality data were used to determine the lethal concentration (LC) values at which 50%, 90%, and 99% of the ticks died (LC(50), LC(90), and LC(99), respectively), and resistance ratios (RR) were calculated based on the LC values. The RR revealed differences between the acaricide-exposed (GYN) and unexposed (PNE) tick strains. The PNE strain larvae and nymphs were susceptible to all the tested acaricides. The GYN strain larvae were tolerant to cypermethrin, whereas the nymphs were tolerant to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal (2 < RR ≤ 10). The GYN strain nymphs were resistant to amitraz (RR > 10). This is the first report of A. sculptum nymphs with resistance to amitraz and tolerance to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal. MDPI 2023-10-31 /pmc/articles/PMC10675591/ /pubmed/38003769 http://dx.doi.org/10.3390/pathogens12111304 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cardoso, Ennya Rafaella Neves
Carvalho, Stephani Félix
Dias, Sarah Alves
Santos, Rayane Almeida
Tavares, Mariana Avelar
Neves, Lucianne Cardoso
Paula, Warley Vieira de Freitas
Pádua, Gracielle Teles
de Lima, Nicolas Jalowitzki
Paludo, Raquel Loren dos Reis
Silva, Isabela Santos
Bittencourt, Raphaela Bueno Mendes
dos Santos, Gabriel Cândido
Nascimento, Flavia Giovana de Jesus
de Paula, Luiza Gabriella Ferreira
Dantas-Torres, Filipe
Monteiro, Caio Marcio De Oliveira
Krawczak, Felipe da Silva
Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title_full Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title_fullStr Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title_full_unstemmed Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title_short Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides
title_sort susceptibility of amblyomma sculptum, vector of rickettsia rickettsii, ticks from a national park and an experimental farm to different synthetic acaricides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10675591/
https://www.ncbi.nlm.nih.gov/pubmed/38003769
http://dx.doi.org/10.3390/pathogens12111304
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