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Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury
[Image: see text] INTRODUCTION: For cell-based therapies of lung injury, several cell sources have been extensively studied. However, the potential of human fetal respiratory cells has not been systematically explored for this purpose. Here, we hypothesize that these cells could be one of the top so...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences (TUOMS Publishing Group)
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676525/ https://www.ncbi.nlm.nih.gov/pubmed/38022383 http://dx.doi.org/10.34172/bi.2023.26456 |
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author | Ganji, Fatemeh Ebrahimi, Marzieh Shirani, Ali Golmohammadi, Mahtab Gholipourmalekabadi, Mazaher Kashanian, Maryam Koolaeinezhad, Kiana Davari, Hamid Reza Mousavi, Seyed Ali Javad Aghayan, Hamid Reza Arjmand, Babak Heshmat, Ramin Karkuki Osguei, Nushin Samadikuchaksaraei, Ali |
author_facet | Ganji, Fatemeh Ebrahimi, Marzieh Shirani, Ali Golmohammadi, Mahtab Gholipourmalekabadi, Mazaher Kashanian, Maryam Koolaeinezhad, Kiana Davari, Hamid Reza Mousavi, Seyed Ali Javad Aghayan, Hamid Reza Arjmand, Babak Heshmat, Ramin Karkuki Osguei, Nushin Samadikuchaksaraei, Ali |
author_sort | Ganji, Fatemeh |
collection | PubMed |
description | [Image: see text] INTRODUCTION: For cell-based therapies of lung injury, several cell sources have been extensively studied. However, the potential of human fetal respiratory cells has not been systematically explored for this purpose. Here, we hypothesize that these cells could be one of the top sources and hence, we extensively updated the definition of their phenotype. METHODS: Human fetal lower respiratory tissues from pseudoglandular and canalicular stages and their isolated epithelial cells were evaluated by immunostaining, electron microscopy, flow cytometry, organoid assay, and gene expression studies. The regenerative potential of the isolated cells has been evaluated in a rat model of bleomycin-induced pulmonary injury by tracheal instillation on days 0 and 14 after injury and harvest of the lungs on day 28. RESULTS: We determined the relative and temporal, and spatial pattern of expression of markers of basal (KRT5, KRT14, TRP63), non-basal (AQP3 and pro-SFTPC), and early progenitor (NKX2.1, SOX2, SOX9) cells. Also, we showed the potential of respiratory-derived cells to contribute to in vitro formation of alveolar and airway-like structures in organoids. Cell therapy decreased fibrosis formation in rat lungs and improved the alveolar structures. It also upregulated the expression of IL-10 (up to 17.22 folds) and surfactant protein C (up to 2.71 folds) and downregulated the expression of TGF-β (up to 5.89 folds) and AQP5 (up to 3.28 folds). CONCLUSION: We provide substantial evidence that human fetal respiratory tract cells can improve the regenerative process after lung injury. Also, our extensive characterization provides an updated phenotypic profile of these cells. |
format | Online Article Text |
id | pubmed-10676525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Tabriz University of Medical Sciences (TUOMS Publishing Group) |
record_format | MEDLINE/PubMed |
spelling | pubmed-106765252023-01-01 Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury Ganji, Fatemeh Ebrahimi, Marzieh Shirani, Ali Golmohammadi, Mahtab Gholipourmalekabadi, Mazaher Kashanian, Maryam Koolaeinezhad, Kiana Davari, Hamid Reza Mousavi, Seyed Ali Javad Aghayan, Hamid Reza Arjmand, Babak Heshmat, Ramin Karkuki Osguei, Nushin Samadikuchaksaraei, Ali Bioimpacts Original Article [Image: see text] INTRODUCTION: For cell-based therapies of lung injury, several cell sources have been extensively studied. However, the potential of human fetal respiratory cells has not been systematically explored for this purpose. Here, we hypothesize that these cells could be one of the top sources and hence, we extensively updated the definition of their phenotype. METHODS: Human fetal lower respiratory tissues from pseudoglandular and canalicular stages and their isolated epithelial cells were evaluated by immunostaining, electron microscopy, flow cytometry, organoid assay, and gene expression studies. The regenerative potential of the isolated cells has been evaluated in a rat model of bleomycin-induced pulmonary injury by tracheal instillation on days 0 and 14 after injury and harvest of the lungs on day 28. RESULTS: We determined the relative and temporal, and spatial pattern of expression of markers of basal (KRT5, KRT14, TRP63), non-basal (AQP3 and pro-SFTPC), and early progenitor (NKX2.1, SOX2, SOX9) cells. Also, we showed the potential of respiratory-derived cells to contribute to in vitro formation of alveolar and airway-like structures in organoids. Cell therapy decreased fibrosis formation in rat lungs and improved the alveolar structures. It also upregulated the expression of IL-10 (up to 17.22 folds) and surfactant protein C (up to 2.71 folds) and downregulated the expression of TGF-β (up to 5.89 folds) and AQP5 (up to 3.28 folds). CONCLUSION: We provide substantial evidence that human fetal respiratory tract cells can improve the regenerative process after lung injury. Also, our extensive characterization provides an updated phenotypic profile of these cells. Tabriz University of Medical Sciences (TUOMS Publishing Group) 2023 2023-07-22 /pmc/articles/PMC10676525/ /pubmed/38022383 http://dx.doi.org/10.34172/bi.2023.26456 Text en © 2023 The Author(s). https://creativecommons.org/licenses/by-nc/4.0/This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Ganji, Fatemeh Ebrahimi, Marzieh Shirani, Ali Golmohammadi, Mahtab Gholipourmalekabadi, Mazaher Kashanian, Maryam Koolaeinezhad, Kiana Davari, Hamid Reza Mousavi, Seyed Ali Javad Aghayan, Hamid Reza Arjmand, Babak Heshmat, Ramin Karkuki Osguei, Nushin Samadikuchaksaraei, Ali Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title | Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title_full | Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title_fullStr | Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title_full_unstemmed | Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title_short | Epithelial cells/progenitor cells in developing human lower respiratory tract: Characterization and transplantation to rat model of pulmonary injury |
title_sort | epithelial cells/progenitor cells in developing human lower respiratory tract: characterization and transplantation to rat model of pulmonary injury |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676525/ https://www.ncbi.nlm.nih.gov/pubmed/38022383 http://dx.doi.org/10.34172/bi.2023.26456 |
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