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713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii

BACKGROUND: The persistence of Acinetobacter on different surfaces and the continuous exposure of bacteria to disinfectants such as quaternary ammonium compounds (QACs) in the healthcare environment have led to microbicide tolerance. Acinetobacter baumannii have also rapidly acquired carbapenem resi...

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Autores principales: Chatterjee, Piyali, Hwang, Munok, Choi, Hosoon, Dhar, Sorabh, Kaye, Keith S, Donskey, Curtis, Jinadatha, Chetan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676967/
http://dx.doi.org/10.1093/ofid/ofad500.775
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author Chatterjee, Piyali
Hwang, Munok
Choi, Hosoon
Dhar, Sorabh
Kaye, Keith S
Donskey, Curtis
Jinadatha, Chetan
author_facet Chatterjee, Piyali
Hwang, Munok
Choi, Hosoon
Dhar, Sorabh
Kaye, Keith S
Donskey, Curtis
Jinadatha, Chetan
author_sort Chatterjee, Piyali
collection PubMed
description BACKGROUND: The persistence of Acinetobacter on different surfaces and the continuous exposure of bacteria to disinfectants such as quaternary ammonium compounds (QACs) in the healthcare environment have led to microbicide tolerance. Acinetobacter baumannii have also rapidly acquired carbapenem resistance. Here we determined the prevalence of microbicide resistance genes in carbapenem susceptible and resistant clinical isolates of Acinetobacter. METHODS: All clinical isolates were collected from two separate hospitals in Detroit between 2017-2021. Following DNA isolation using Qiagen kit, Nextera Flex kit was used for library preparation of Acinetobacter clinical isolates. Whole genome sequencing (WGS) was performed via Illumina NextSeq 550 using the prepared DNA library. The FASTQ files from the sequencing run were then subjected to bioinformatic analysis with Bionumerics software v7.6. ResFinder was used for determination of presence or absence of QAC resistant genes and results were tabulated by Carbapenem-Resistant Acinetobacter baumannii (CRAb) or Carbapenem-Susceptible Acinetobacter baumannii (CSAb) with or without the QAC resistant genes. RESULTS: Out of the 139 Acinetobacter patient isolates, 70/139 (50.4%) were CRAb containing carbapenemase blaOXA-23 and 69/139 (49.6%) belonged to CSAb (Table 1). Figure 1. shows the prevalence of antimicrobial resistance (AMR) genes in CRAb and CSAb isolates with qacE genes. For CRAb isolates, 56% had qacE genes while 39% of CSAb had qacE genes. About 42% of CRAb isolates were multi-drug resistant (MDR), indicating a prevalence of qacE genes among the MDR isolates. Table 2 shows the presence of qacE genes by sequence type (ST). In the ST2 (Pasteur) and ST406 (Pasteur) strains, qacE gene was mainly present. Interestingly, most of the Acinetobacter isolates that belong to ST2 (ST195 and ST281, Oxford) are CRAb with qacE gene. [Figure: see text] [Figure: see text] [Figure: see text] CONCLUSION: Clinical isolates in our study had an even distribution of both CRAb and CSAb. The presence of qacE gene was more prevalent in the CRAb isolates compared to the CSAb isolates. While different STs harbored the qacE gene, the prevalence of qacE gene was highest in ST2 among all isolates. Future studies are needed to determine the reduced susceptibility to other commonly used hospital disinfectants. DISCLOSURES: Keith S. Kaye, MD, MPH, Abbvie: Advisor/Consultant|Abbvie: Honoraria|Entasis: Advisor/Consultant|Entasis: Honoraria|GSK: Advisor/Consultant|GSK: Honoraria|Merck: Advisor/Consultant|Merck: Honoraria|Shionogi: Advisor/Consultant|Shionogi: Honoraria|VenatoRx: Advisor/Consultant|VenatoRx: Honoraria
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spelling pubmed-106769672023-11-27 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii Chatterjee, Piyali Hwang, Munok Choi, Hosoon Dhar, Sorabh Kaye, Keith S Donskey, Curtis Jinadatha, Chetan Open Forum Infect Dis Abstract BACKGROUND: The persistence of Acinetobacter on different surfaces and the continuous exposure of bacteria to disinfectants such as quaternary ammonium compounds (QACs) in the healthcare environment have led to microbicide tolerance. Acinetobacter baumannii have also rapidly acquired carbapenem resistance. Here we determined the prevalence of microbicide resistance genes in carbapenem susceptible and resistant clinical isolates of Acinetobacter. METHODS: All clinical isolates were collected from two separate hospitals in Detroit between 2017-2021. Following DNA isolation using Qiagen kit, Nextera Flex kit was used for library preparation of Acinetobacter clinical isolates. Whole genome sequencing (WGS) was performed via Illumina NextSeq 550 using the prepared DNA library. The FASTQ files from the sequencing run were then subjected to bioinformatic analysis with Bionumerics software v7.6. ResFinder was used for determination of presence or absence of QAC resistant genes and results were tabulated by Carbapenem-Resistant Acinetobacter baumannii (CRAb) or Carbapenem-Susceptible Acinetobacter baumannii (CSAb) with or without the QAC resistant genes. RESULTS: Out of the 139 Acinetobacter patient isolates, 70/139 (50.4%) were CRAb containing carbapenemase blaOXA-23 and 69/139 (49.6%) belonged to CSAb (Table 1). Figure 1. shows the prevalence of antimicrobial resistance (AMR) genes in CRAb and CSAb isolates with qacE genes. For CRAb isolates, 56% had qacE genes while 39% of CSAb had qacE genes. About 42% of CRAb isolates were multi-drug resistant (MDR), indicating a prevalence of qacE genes among the MDR isolates. Table 2 shows the presence of qacE genes by sequence type (ST). In the ST2 (Pasteur) and ST406 (Pasteur) strains, qacE gene was mainly present. Interestingly, most of the Acinetobacter isolates that belong to ST2 (ST195 and ST281, Oxford) are CRAb with qacE gene. [Figure: see text] [Figure: see text] [Figure: see text] CONCLUSION: Clinical isolates in our study had an even distribution of both CRAb and CSAb. The presence of qacE gene was more prevalent in the CRAb isolates compared to the CSAb isolates. While different STs harbored the qacE gene, the prevalence of qacE gene was highest in ST2 among all isolates. Future studies are needed to determine the reduced susceptibility to other commonly used hospital disinfectants. DISCLOSURES: Keith S. Kaye, MD, MPH, Abbvie: Advisor/Consultant|Abbvie: Honoraria|Entasis: Advisor/Consultant|Entasis: Honoraria|GSK: Advisor/Consultant|GSK: Honoraria|Merck: Advisor/Consultant|Merck: Honoraria|Shionogi: Advisor/Consultant|Shionogi: Honoraria|VenatoRx: Advisor/Consultant|VenatoRx: Honoraria Oxford University Press 2023-11-27 /pmc/articles/PMC10676967/ http://dx.doi.org/10.1093/ofid/ofad500.775 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Abstract
Chatterjee, Piyali
Hwang, Munok
Choi, Hosoon
Dhar, Sorabh
Kaye, Keith S
Donskey, Curtis
Jinadatha, Chetan
713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title_full 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title_fullStr 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title_full_unstemmed 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title_short 713. Prevalence of Microbicide Resistance Genes in Clinical Isolates of Acinetobacter baumannii
title_sort 713. prevalence of microbicide resistance genes in clinical isolates of acinetobacter baumannii
topic Abstract
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676967/
http://dx.doi.org/10.1093/ofid/ofad500.775
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