Cargando…
1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes
BACKGROUND: Current methods for reprocessing duodenoscopes, including double high-level disinfection, are not effective in eliminating bacterial contamination. Thus, there is a need to develop effective and safe sterilization technologies for duodenoscopes. METHODS: We examined the effectiveness of...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10678175/ http://dx.doi.org/10.1093/ofid/ofad500.1256 |
_version_ | 1785150300405891072 |
---|---|
author | Varghese, Martin M Cadnum, Jennifer Memic, Samir Torres-Teran, Maria M Rutala, William Donskey, Curtis |
author_facet | Varghese, Martin M Cadnum, Jennifer Memic, Samir Torres-Teran, Maria M Rutala, William Donskey, Curtis |
author_sort | Varghese, Martin M |
collection | PubMed |
description | BACKGROUND: Current methods for reprocessing duodenoscopes, including double high-level disinfection, are not effective in eliminating bacterial contamination. Thus, there is a need to develop effective and safe sterilization technologies for duodenoscopes. METHODS: We examined the effectiveness of a new hydrogen peroxide gas plasma sterilizer designed for terminal sterilization of flexible endoscopes. The technology directs vaporized hydrogen peroxide into all internal endoscope channels with a short exposure time and includes a sterilization container that facilitates sterile storage after processing. Test organisms included Bacillus atrophaeus and Clostridioides difficile spores, Escherichia coli, Enterococcus faecium, and Candida auris inoculated on steel wires or dispersed throughout the internal channels with 5% fetal calf serum. RESULTS: The technology reduced >10(8) colony-forming units (CFU) of the vegetative organisms to undetectable levels. B. atrophaeus and C. difficile spores on steel wires were eliminated by the technology ( >6 log(10) CFU reduction) and spores disperse throughout the channels were reduced by >5 log(10) CFU. No damage to the endoscopes was observed. CONCLUSION: The sterilization technology was highly effective in reducing contamination of duodenoscopes in laboratory testing. Our results support further investigation of this technology in reducing contamination of in-use endoscopes. DISCLOSURES: William Rutala, MS, MPH, PhD, Ideate Medical: Advisor/Consultant|Ideate Medical: Stocks/Bonds|Kinnos: Advisor/Consultant|Kinnos: Stocks/Bonds|PDI: Advisor/Consultant|PDI: Honoraria |
format | Online Article Text |
id | pubmed-10678175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-106781752023-11-27 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes Varghese, Martin M Cadnum, Jennifer Memic, Samir Torres-Teran, Maria M Rutala, William Donskey, Curtis Open Forum Infect Dis Abstract BACKGROUND: Current methods for reprocessing duodenoscopes, including double high-level disinfection, are not effective in eliminating bacterial contamination. Thus, there is a need to develop effective and safe sterilization technologies for duodenoscopes. METHODS: We examined the effectiveness of a new hydrogen peroxide gas plasma sterilizer designed for terminal sterilization of flexible endoscopes. The technology directs vaporized hydrogen peroxide into all internal endoscope channels with a short exposure time and includes a sterilization container that facilitates sterile storage after processing. Test organisms included Bacillus atrophaeus and Clostridioides difficile spores, Escherichia coli, Enterococcus faecium, and Candida auris inoculated on steel wires or dispersed throughout the internal channels with 5% fetal calf serum. RESULTS: The technology reduced >10(8) colony-forming units (CFU) of the vegetative organisms to undetectable levels. B. atrophaeus and C. difficile spores on steel wires were eliminated by the technology ( >6 log(10) CFU reduction) and spores disperse throughout the channels were reduced by >5 log(10) CFU. No damage to the endoscopes was observed. CONCLUSION: The sterilization technology was highly effective in reducing contamination of duodenoscopes in laboratory testing. Our results support further investigation of this technology in reducing contamination of in-use endoscopes. DISCLOSURES: William Rutala, MS, MPH, PhD, Ideate Medical: Advisor/Consultant|Ideate Medical: Stocks/Bonds|Kinnos: Advisor/Consultant|Kinnos: Stocks/Bonds|PDI: Advisor/Consultant|PDI: Honoraria Oxford University Press 2023-11-27 /pmc/articles/PMC10678175/ http://dx.doi.org/10.1093/ofid/ofad500.1256 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Abstract Varghese, Martin M Cadnum, Jennifer Memic, Samir Torres-Teran, Maria M Rutala, William Donskey, Curtis 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title | 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title_full | 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title_fullStr | 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title_full_unstemmed | 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title_short | 1419. Evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
title_sort | 1419. evaluation of a vaporized hydrogen peroxide sterilization technology for duodenoscopes |
topic | Abstract |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10678175/ http://dx.doi.org/10.1093/ofid/ofad500.1256 |
work_keys_str_mv | AT varghesemartinm 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes AT cadnumjennifer 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes AT memicsamir 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes AT torresteranmariam 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes AT rutalawilliam 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes AT donskeycurtis 1419evaluationofavaporizedhydrogenperoxidesterilizationtechnologyforduodenoscopes |