593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales

BACKGROUND: The rapid and wide spread of carbapenemase-producing Enterobacterales (CPE) has become a global concern. Currently, β-lactams with β-lactamase inhibitors (such as ceftazidime/avibactam, relebactam/imipenem) are used for treatment of CPE infection as one of the effective agents. However,...

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Autores principales: Sakanashi, Daisuke, Suematsu, Hiroyuki, Takayama, Mina, Kawamoto, Yuzuka, Miyazaki, Narimi, Ohno, Tomoko, Yamada, Atsuko, Nakamura, Akiko, Ohta, Hirotoshi, Koita, Isao, Hirai, Jun, Asai, Nobuhiro, Mori, Nobuaki, Mikamo, Hiroshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10678555/
http://dx.doi.org/10.1093/ofid/ofad500.661
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author Sakanashi, Daisuke
Suematsu, Hiroyuki
Takayama, Mina
Kawamoto, Yuzuka
Miyazaki, Narimi
Ohno, Tomoko
Yamada, Atsuko
Nakamura, Akiko
Ohta, Hirotoshi
Koita, Isao
Hirai, Jun
Asai, Nobuhiro
Mori, Nobuaki
Mikamo, Hiroshige
author_facet Sakanashi, Daisuke
Suematsu, Hiroyuki
Takayama, Mina
Kawamoto, Yuzuka
Miyazaki, Narimi
Ohno, Tomoko
Yamada, Atsuko
Nakamura, Akiko
Ohta, Hirotoshi
Koita, Isao
Hirai, Jun
Asai, Nobuhiro
Mori, Nobuaki
Mikamo, Hiroshige
author_sort Sakanashi, Daisuke
collection PubMed
description BACKGROUND: The rapid and wide spread of carbapenemase-producing Enterobacterales (CPE) has become a global concern. Currently, β-lactams with β-lactamase inhibitors (such as ceftazidime/avibactam, relebactam/imipenem) are used for treatment of CPE infection as one of the effective agents. However, some of combinations do not have activity against MBLs. Thus, we believe that the discriminating between MBL and serine-CPE is important. The eCIM has been published by Clinical & Laboratory Standards Institute (CLSI) to differentiate to MBL from serine-CPE. The eCIM is required EDTA, that is not clinical diagnostic agent. In this study, we examined simplifying the eCIM procedure by using the EDTA blood collection tube (BCT-eCIM). METHODS: A total of 53 CPE (39 MBL and 14 Serin-CPE) were tested. The test population included 39 clinical isolates, and 14 reference strains from NCTC or ATCC (Table 1). The eCIM were performed and interpreted by following CLSI procedures. For BCT-eCIM, whole quantity of 2-mL TSB was inoculated into a BD Vacutainer EDTA Tube (2-mL) (Becton-Dickinson) (Vacutainer-eCIM) or an EDTA-2K Insepack II-D 2-mL tube (Kyokuto Pharmaceutical Industrial, Tokyo, Japan) (Insepack-eCIM); the tubes conform to International Organization for Standardization 6710, hence the 2-mL TSB inoculated tubes obtain final concentration of 4.1 to 6.8 mM EDTA. The BCT-eCIM used these tubes as a substitute for 0.5 mM EDTA TSB tube of eCIM, and any other procedures followed the eCIM procedure. Additionally, we performed the 4 mM- and 7 mM-eCIM to verify the effect of EDTA concentration (variable region of BCT-eCIM). Table 1 [Figure: see text] Bacterial strains RESULTS: Table 2 and Figure 1 show the performance data of each eCIMs and the positive and negative Quality control results, respectively. The sensitivity of 4 mM-eCIM, 5 mM-eCIM, 7 mM-eCIM, Vacutainer-eCIM and Insepack-eCIM were 37/40 (93%), 36/40 (90%), 36/40 (90%), 39/40 (98%) and 39/40 (98%), respectively. The specificity of 4 mM-eCIM, 5 mM-eCIM, 7 mM-eCIM, Vacutainer-eCIM and Insepack-eCIM were 11/13 (85%), 13/13 (100%), 13/13 (100%), 12/13 (92%) and 12/13 (92%), respectively. Table 2 [Figure: see text] Results obtained using the eCIM and BCT-eCIM [Figure: see text] CONCLUSION: Our methods (BCT-eCIMs) were superior to sensitivity compared to conventional eCIMs. Therefore, BCT-eCIM may be useful in clinical settings as the simplifying method. DISCLOSURES: Hiroshige Mikamo, M.D, Ph.D, Asahi Kasei Pharma Corporation: Grant/Research Support|Merck Sharp & Dohme: Honoraria|Pfizer Inc.: Grant/Research Support|Pfizer R&D Japan: Honoraria|Sumitomo Pharma Co., Ltd.: Grant/Research Support|Sumitomo Pharma Co., Ltd.: Honoraria
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spelling pubmed-106785552023-11-27 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales Sakanashi, Daisuke Suematsu, Hiroyuki Takayama, Mina Kawamoto, Yuzuka Miyazaki, Narimi Ohno, Tomoko Yamada, Atsuko Nakamura, Akiko Ohta, Hirotoshi Koita, Isao Hirai, Jun Asai, Nobuhiro Mori, Nobuaki Mikamo, Hiroshige Open Forum Infect Dis Abstract BACKGROUND: The rapid and wide spread of carbapenemase-producing Enterobacterales (CPE) has become a global concern. Currently, β-lactams with β-lactamase inhibitors (such as ceftazidime/avibactam, relebactam/imipenem) are used for treatment of CPE infection as one of the effective agents. However, some of combinations do not have activity against MBLs. Thus, we believe that the discriminating between MBL and serine-CPE is important. The eCIM has been published by Clinical & Laboratory Standards Institute (CLSI) to differentiate to MBL from serine-CPE. The eCIM is required EDTA, that is not clinical diagnostic agent. In this study, we examined simplifying the eCIM procedure by using the EDTA blood collection tube (BCT-eCIM). METHODS: A total of 53 CPE (39 MBL and 14 Serin-CPE) were tested. The test population included 39 clinical isolates, and 14 reference strains from NCTC or ATCC (Table 1). The eCIM were performed and interpreted by following CLSI procedures. For BCT-eCIM, whole quantity of 2-mL TSB was inoculated into a BD Vacutainer EDTA Tube (2-mL) (Becton-Dickinson) (Vacutainer-eCIM) or an EDTA-2K Insepack II-D 2-mL tube (Kyokuto Pharmaceutical Industrial, Tokyo, Japan) (Insepack-eCIM); the tubes conform to International Organization for Standardization 6710, hence the 2-mL TSB inoculated tubes obtain final concentration of 4.1 to 6.8 mM EDTA. The BCT-eCIM used these tubes as a substitute for 0.5 mM EDTA TSB tube of eCIM, and any other procedures followed the eCIM procedure. Additionally, we performed the 4 mM- and 7 mM-eCIM to verify the effect of EDTA concentration (variable region of BCT-eCIM). Table 1 [Figure: see text] Bacterial strains RESULTS: Table 2 and Figure 1 show the performance data of each eCIMs and the positive and negative Quality control results, respectively. The sensitivity of 4 mM-eCIM, 5 mM-eCIM, 7 mM-eCIM, Vacutainer-eCIM and Insepack-eCIM were 37/40 (93%), 36/40 (90%), 36/40 (90%), 39/40 (98%) and 39/40 (98%), respectively. The specificity of 4 mM-eCIM, 5 mM-eCIM, 7 mM-eCIM, Vacutainer-eCIM and Insepack-eCIM were 11/13 (85%), 13/13 (100%), 13/13 (100%), 12/13 (92%) and 12/13 (92%), respectively. Table 2 [Figure: see text] Results obtained using the eCIM and BCT-eCIM [Figure: see text] CONCLUSION: Our methods (BCT-eCIMs) were superior to sensitivity compared to conventional eCIMs. Therefore, BCT-eCIM may be useful in clinical settings as the simplifying method. DISCLOSURES: Hiroshige Mikamo, M.D, Ph.D, Asahi Kasei Pharma Corporation: Grant/Research Support|Merck Sharp & Dohme: Honoraria|Pfizer Inc.: Grant/Research Support|Pfizer R&D Japan: Honoraria|Sumitomo Pharma Co., Ltd.: Grant/Research Support|Sumitomo Pharma Co., Ltd.: Honoraria Oxford University Press 2023-11-27 /pmc/articles/PMC10678555/ http://dx.doi.org/10.1093/ofid/ofad500.661 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Abstract
Sakanashi, Daisuke
Suematsu, Hiroyuki
Takayama, Mina
Kawamoto, Yuzuka
Miyazaki, Narimi
Ohno, Tomoko
Yamada, Atsuko
Nakamura, Akiko
Ohta, Hirotoshi
Koita, Isao
Hirai, Jun
Asai, Nobuhiro
Mori, Nobuaki
Mikamo, Hiroshige
593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title_full 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title_fullStr 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title_full_unstemmed 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title_short 593. Evaluation of the EDTA-modified Carbapenem Inactivation Method (eCIM) with Using the EDTA Blood Collection Tube for Differentiating the Serine- and Metallo-β-lactamase (MBL) Producing Enterobacterales
title_sort 593. evaluation of the edta-modified carbapenem inactivation method (ecim) with using the edta blood collection tube for differentiating the serine- and metallo-β-lactamase (mbl) producing enterobacterales
topic Abstract
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10678555/
http://dx.doi.org/10.1093/ofid/ofad500.661
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