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Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells
Graphene and its derivatives have attracted much attention as nanomaterials in bone tissue engineering because of their remarkable ability to induce cell osteogenic differentiation. However, graphene quantum dots (GQDs), as graphene derivatives, little is known about their osteodifferentiation- and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679356/ https://www.ncbi.nlm.nih.gov/pubmed/38025053 http://dx.doi.org/10.3389/fchem.2023.1213507 |
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author | Liang, Yan Gao, Wanshan Deng, Sicheng Wu, Dongyan Jiang, Yiling Zhang, Yishan Qiu, Rongmin |
author_facet | Liang, Yan Gao, Wanshan Deng, Sicheng Wu, Dongyan Jiang, Yiling Zhang, Yishan Qiu, Rongmin |
author_sort | Liang, Yan |
collection | PubMed |
description | Graphene and its derivatives have attracted much attention as nanomaterials in bone tissue engineering because of their remarkable ability to induce cell osteogenic differentiation. However, graphene quantum dots (GQDs), as graphene derivatives, little is known about their osteodifferentiation- and osteoinduction-promoting capabilities, especially in the restoration of bone defect caused by periodontitis. Therefore, there is a growing need to investigate the effect of GQDs on periodontal ligament stem cells (PDLSCs). Here, we postulated that GQDs are a promising biocompatible nanomaterial that facilitated the migration and differentiation of PDLSCs, and use laboratory methods like CCK-8, transwell experiments, qRT-PCR, Alizarin red staining and immunofluorescence staining to evaluate. Our experiments confirmed that GQDs did not inhibit cell viability, with most cells remaining viable even at GQDs concentrations of up to 30 μg mL(-1). Moreover, GQDs were found to significantly enhance PDLSC migration, with the peak effect observed at concentrations of 5 and 10 μg mL(-1). Furthermore, GQDs accelerated osteoblastic differentiation in PDLSCs and induced the mineralization of calcium nodules. Additionally, GQDs were shown to promote fibroblast differentiation in PDLSCs compared to the control group. Thus, GQDs not only possessed low cytotoxicity and good biocompatibility, but also displayed the beneficial capability to migration and differentiation of PDLSCs, which indicated GQDs might be a potential nanomaterial for bone regeneration. |
format | Online Article Text |
id | pubmed-10679356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106793562023-01-01 Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells Liang, Yan Gao, Wanshan Deng, Sicheng Wu, Dongyan Jiang, Yiling Zhang, Yishan Qiu, Rongmin Front Chem Chemistry Graphene and its derivatives have attracted much attention as nanomaterials in bone tissue engineering because of their remarkable ability to induce cell osteogenic differentiation. However, graphene quantum dots (GQDs), as graphene derivatives, little is known about their osteodifferentiation- and osteoinduction-promoting capabilities, especially in the restoration of bone defect caused by periodontitis. Therefore, there is a growing need to investigate the effect of GQDs on periodontal ligament stem cells (PDLSCs). Here, we postulated that GQDs are a promising biocompatible nanomaterial that facilitated the migration and differentiation of PDLSCs, and use laboratory methods like CCK-8, transwell experiments, qRT-PCR, Alizarin red staining and immunofluorescence staining to evaluate. Our experiments confirmed that GQDs did not inhibit cell viability, with most cells remaining viable even at GQDs concentrations of up to 30 μg mL(-1). Moreover, GQDs were found to significantly enhance PDLSC migration, with the peak effect observed at concentrations of 5 and 10 μg mL(-1). Furthermore, GQDs accelerated osteoblastic differentiation in PDLSCs and induced the mineralization of calcium nodules. Additionally, GQDs were shown to promote fibroblast differentiation in PDLSCs compared to the control group. Thus, GQDs not only possessed low cytotoxicity and good biocompatibility, but also displayed the beneficial capability to migration and differentiation of PDLSCs, which indicated GQDs might be a potential nanomaterial for bone regeneration. Frontiers Media S.A. 2023-11-13 /pmc/articles/PMC10679356/ /pubmed/38025053 http://dx.doi.org/10.3389/fchem.2023.1213507 Text en Copyright © 2023 Liang, Gao, Deng, Wu, Jiang, Zhang and Qiu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Liang, Yan Gao, Wanshan Deng, Sicheng Wu, Dongyan Jiang, Yiling Zhang, Yishan Qiu, Rongmin Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title | Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title_full | Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title_fullStr | Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title_full_unstemmed | Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title_short | Graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
title_sort | graphene quantum dots promote migration and differentiation of periodontal ligament stem cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679356/ https://www.ncbi.nlm.nih.gov/pubmed/38025053 http://dx.doi.org/10.3389/fchem.2023.1213507 |
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