Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells

Several recombinant proteins have been successfully produced in plants. This usually requires Agrobacterium-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery v...

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Autores principales: Navarre, Catherine, Orval, Rik, Peeters, Marie, Bailly, Nicolas, Chaumont, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679441/
https://www.ncbi.nlm.nih.gov/pubmed/38023881
http://dx.doi.org/10.3389/fpls.2023.1266775
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author Navarre, Catherine
Orval, Rik
Peeters, Marie
Bailly, Nicolas
Chaumont, François
author_facet Navarre, Catherine
Orval, Rik
Peeters, Marie
Bailly, Nicolas
Chaumont, François
author_sort Navarre, Catherine
collection PubMed
description Several recombinant proteins have been successfully produced in plants. This usually requires Agrobacterium-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via A. tumefaciens, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of A. tumefaciens LBA4404 into the expression cassette when using the widely adopted 35S promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in Nicotiana tabacum BY-2 suspension cells.
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spelling pubmed-106794412023-01-01 Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells Navarre, Catherine Orval, Rik Peeters, Marie Bailly, Nicolas Chaumont, François Front Plant Sci Plant Science Several recombinant proteins have been successfully produced in plants. This usually requires Agrobacterium-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via A. tumefaciens, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of A. tumefaciens LBA4404 into the expression cassette when using the widely adopted 35S promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in Nicotiana tabacum BY-2 suspension cells. Frontiers Media S.A. 2023-11-13 /pmc/articles/PMC10679441/ /pubmed/38023881 http://dx.doi.org/10.3389/fpls.2023.1266775 Text en Copyright © 2023 Navarre, Orval, Peeters, Bailly and Chaumont https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Navarre, Catherine
Orval, Rik
Peeters, Marie
Bailly, Nicolas
Chaumont, François
Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title_full Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title_fullStr Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title_full_unstemmed Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title_short Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
title_sort issue when expressing a recombinant protein under the control of p35s in nicotiana tabacum by-2 cells
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679441/
https://www.ncbi.nlm.nih.gov/pubmed/38023881
http://dx.doi.org/10.3389/fpls.2023.1266775
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