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Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling

Sunflower (Helianthus annuus L.) is the second most important oil seed crop in Europe. The seeds are used as confection seeds and, more importantly, to generate an edible vegetable oil, which in normal varieties is rich in the polyunsaturated fatty acid linoleic acid. Linoleic acid is biosynthesized...

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Autores principales: Rozhon, Wilfried, Ramirez, Veronica E., Wieckhorst, Silke, Hahn, Volker, Poppenberger, Brigitte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679672/
https://www.ncbi.nlm.nih.gov/pubmed/38023891
http://dx.doi.org/10.3389/fpls.2023.1138603
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author Rozhon, Wilfried
Ramirez, Veronica E.
Wieckhorst, Silke
Hahn, Volker
Poppenberger, Brigitte
author_facet Rozhon, Wilfried
Ramirez, Veronica E.
Wieckhorst, Silke
Hahn, Volker
Poppenberger, Brigitte
author_sort Rozhon, Wilfried
collection PubMed
description Sunflower (Helianthus annuus L.) is the second most important oil seed crop in Europe. The seeds are used as confection seeds and, more importantly, to generate an edible vegetable oil, which in normal varieties is rich in the polyunsaturated fatty acid linoleic acid. Linoleic acid is biosynthesized from oleic acid through activity of the oleate desaturase FATTY ACID DESATURASE 2 (FAD2), which in seeds is encoded by FAD2-1, a gene that’s present in single copy in sunflowers. Defective FAD2-1 expression enriches oleic acid, yielding the high oleic (HO) acid trait, which is of great interest in oil seed crops, since HO oil bears benefits for both food and non-food applications. Chemical mutagenesis has previously been used to generate sunflower mutants with reduced FAD2-1 expression and here it was aimed to produce further genetic material in which FAD2-1 activity is lost and the HO trait is stably expressed. For this purpose, a sunflower mutant population was created using gamma irradiation and screened for fad2-1 mutants with a newly developed HPLC-based fatty-acid profiling system that’s suitable for high-throughput analyses. With this approach fad2-1 knock-out mutants could be isolated, which stably hyper-accumulate oleic acid in concentrations of 85-90% of the total fatty acid pool. The genetic nature of these new sunflower lines was characterized and will facilitate marker development, for the rapid introgression of the trait into elite sunflower breeding material.
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spelling pubmed-106796722023-01-01 Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling Rozhon, Wilfried Ramirez, Veronica E. Wieckhorst, Silke Hahn, Volker Poppenberger, Brigitte Front Plant Sci Plant Science Sunflower (Helianthus annuus L.) is the second most important oil seed crop in Europe. The seeds are used as confection seeds and, more importantly, to generate an edible vegetable oil, which in normal varieties is rich in the polyunsaturated fatty acid linoleic acid. Linoleic acid is biosynthesized from oleic acid through activity of the oleate desaturase FATTY ACID DESATURASE 2 (FAD2), which in seeds is encoded by FAD2-1, a gene that’s present in single copy in sunflowers. Defective FAD2-1 expression enriches oleic acid, yielding the high oleic (HO) acid trait, which is of great interest in oil seed crops, since HO oil bears benefits for both food and non-food applications. Chemical mutagenesis has previously been used to generate sunflower mutants with reduced FAD2-1 expression and here it was aimed to produce further genetic material in which FAD2-1 activity is lost and the HO trait is stably expressed. For this purpose, a sunflower mutant population was created using gamma irradiation and screened for fad2-1 mutants with a newly developed HPLC-based fatty-acid profiling system that’s suitable for high-throughput analyses. With this approach fad2-1 knock-out mutants could be isolated, which stably hyper-accumulate oleic acid in concentrations of 85-90% of the total fatty acid pool. The genetic nature of these new sunflower lines was characterized and will facilitate marker development, for the rapid introgression of the trait into elite sunflower breeding material. Frontiers Media S.A. 2023-11-13 /pmc/articles/PMC10679672/ /pubmed/38023891 http://dx.doi.org/10.3389/fpls.2023.1138603 Text en Copyright © 2023 Rozhon, Ramirez, Wieckhorst, Hahn and Poppenberger https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Rozhon, Wilfried
Ramirez, Veronica E.
Wieckhorst, Silke
Hahn, Volker
Poppenberger, Brigitte
Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title_full Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title_fullStr Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title_full_unstemmed Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title_short Generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
title_sort generation of high oleic acid sunflower lines using gamma radiation mutagenesis and high-throughput fatty acid profiling
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679672/
https://www.ncbi.nlm.nih.gov/pubmed/38023891
http://dx.doi.org/10.3389/fpls.2023.1138603
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