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Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos

A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipi...

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Autores principales: Nakajima, Yuki, Tagami, Takahiro, Tajima, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679837/
https://www.ncbi.nlm.nih.gov/pubmed/38034482
http://dx.doi.org/10.2141/jpsa.2023028
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author Nakajima, Yuki
Tagami, Takahiro
Tajima, Atsushi
author_facet Nakajima, Yuki
Tagami, Takahiro
Tajima, Atsushi
author_sort Nakajima, Yuki
collection PubMed
description A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes.
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spelling pubmed-106798372023-11-30 Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos Nakajima, Yuki Tagami, Takahiro Tajima, Atsushi J Poult Sci Full Paper A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes. Japan Poultry Science Association 2023-11-29 /pmc/articles/PMC10679837/ /pubmed/38034482 http://dx.doi.org/10.2141/jpsa.2023028 Text en 2023 Japan Poultry Science Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.
spellingShingle Full Paper
Nakajima, Yuki
Tagami, Takahiro
Tajima, Atsushi
Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title_full Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title_fullStr Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title_full_unstemmed Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title_short Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
title_sort gonadal germ cell migration and proliferation after transfer in developing chicken embryos
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679837/
https://www.ncbi.nlm.nih.gov/pubmed/38034482
http://dx.doi.org/10.2141/jpsa.2023028
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