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Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos
A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Japan Poultry Science Association
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679837/ https://www.ncbi.nlm.nih.gov/pubmed/38034482 http://dx.doi.org/10.2141/jpsa.2023028 |
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author | Nakajima, Yuki Tagami, Takahiro Tajima, Atsushi |
author_facet | Nakajima, Yuki Tagami, Takahiro Tajima, Atsushi |
author_sort | Nakajima, Yuki |
collection | PubMed |
description | A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes. |
format | Online Article Text |
id | pubmed-10679837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Japan Poultry Science Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-106798372023-11-30 Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos Nakajima, Yuki Tagami, Takahiro Tajima, Atsushi J Poult Sci Full Paper A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes. Japan Poultry Science Association 2023-11-29 /pmc/articles/PMC10679837/ /pubmed/38034482 http://dx.doi.org/10.2141/jpsa.2023028 Text en 2023 Japan Poultry Science Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License. |
spellingShingle | Full Paper Nakajima, Yuki Tagami, Takahiro Tajima, Atsushi Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos |
title | Gonadal Germ Cell Migration and Proliferation after Transfer in Developing
Chicken Embryos |
title_full | Gonadal Germ Cell Migration and Proliferation after Transfer in Developing
Chicken Embryos |
title_fullStr | Gonadal Germ Cell Migration and Proliferation after Transfer in Developing
Chicken Embryos |
title_full_unstemmed | Gonadal Germ Cell Migration and Proliferation after Transfer in Developing
Chicken Embryos |
title_short | Gonadal Germ Cell Migration and Proliferation after Transfer in Developing
Chicken Embryos |
title_sort | gonadal germ cell migration and proliferation after transfer in developing
chicken embryos |
topic | Full Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679837/ https://www.ncbi.nlm.nih.gov/pubmed/38034482 http://dx.doi.org/10.2141/jpsa.2023028 |
work_keys_str_mv | AT nakajimayuki gonadalgermcellmigrationandproliferationaftertransferindevelopingchickenembryos AT tagamitakahiro gonadalgermcellmigrationandproliferationaftertransferindevelopingchickenembryos AT tajimaatsushi gonadalgermcellmigrationandproliferationaftertransferindevelopingchickenembryos |