Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction

Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sor...

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Detalles Bibliográficos
Autores principales: Liu, Qianglin, Li, Chaoyang, Li, Yuxia, Wang, Leshan, Zhang, Xujia, Deng, Buhao, Gao, Peidong, Shiri, Mohammad, Alkaifi, Fozi, Zhao, Junxing, Stephens, Jacqueline M., Simintiras, Constantine A., Francis, Joseph, Sun, Jiangwen, Fu, Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679935/
https://www.ncbi.nlm.nih.gov/pubmed/37948186
http://dx.doi.org/10.1016/j.xpro.2023.102703
Descripción
Sumario:Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).(1)

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