Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction

Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sor...

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Detalles Bibliográficos
Autores principales: Liu, Qianglin, Li, Chaoyang, Li, Yuxia, Wang, Leshan, Zhang, Xujia, Deng, Buhao, Gao, Peidong, Shiri, Mohammad, Alkaifi, Fozi, Zhao, Junxing, Stephens, Jacqueline M., Simintiras, Constantine A., Francis, Joseph, Sun, Jiangwen, Fu, Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679935/
https://www.ncbi.nlm.nih.gov/pubmed/37948186
http://dx.doi.org/10.1016/j.xpro.2023.102703

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