Time-lapse live-cell imaging of pyroptosis by confocal microscopy

Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic...

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Detalles Bibliográficos
Autores principales: Jiang, Shuai, Qin, Kunpeng, Sun, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679943/
https://www.ncbi.nlm.nih.gov/pubmed/37948183
http://dx.doi.org/10.1016/j.xpro.2023.102708
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author Jiang, Shuai
Qin, Kunpeng
Sun, Li
author_facet Jiang, Shuai
Qin, Kunpeng
Sun, Li
author_sort Jiang, Shuai
collection PubMed
description Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).(1)
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spelling pubmed-106799432023-11-09 Time-lapse live-cell imaging of pyroptosis by confocal microscopy Jiang, Shuai Qin, Kunpeng Sun, Li STAR Protoc Protocol Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).(1) Elsevier 2023-11-09 /pmc/articles/PMC10679943/ /pubmed/37948183 http://dx.doi.org/10.1016/j.xpro.2023.102708 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jiang, Shuai
Qin, Kunpeng
Sun, Li
Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title_full Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title_fullStr Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title_full_unstemmed Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title_short Time-lapse live-cell imaging of pyroptosis by confocal microscopy
title_sort time-lapse live-cell imaging of pyroptosis by confocal microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679943/
https://www.ncbi.nlm.nih.gov/pubmed/37948183
http://dx.doi.org/10.1016/j.xpro.2023.102708
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AT qinkunpeng timelapselivecellimagingofpyroptosisbyconfocalmicroscopy
AT sunli timelapselivecellimagingofpyroptosisbyconfocalmicroscopy

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