Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies

The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis stud...

Descripción completa

Detalles Bibliográficos
Autores principales: Giannou, Anastasios D., Ohm, Birte, Zazara, Dimitra E., Lücke, Jöran, Zhang, Tao, Sabihi, Morsal, Seeger, Philipp, Oh, Jun, Grotelüschen, Rainer, Busch, Philipp, Mann, Oliver, Hackert, Thilo, Izbicki, Jakob R., Yamada, Yoshito, Huber, Samuel, Jungraithmayr, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679946/
https://www.ncbi.nlm.nih.gov/pubmed/37950863
http://dx.doi.org/10.1016/j.xpro.2023.102701
Descripción
Sumario:The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).(1)

Ejemplares similares