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Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies
The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis stud...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679946/ https://www.ncbi.nlm.nih.gov/pubmed/37950863 http://dx.doi.org/10.1016/j.xpro.2023.102701 |
| _version_ | 1785142283353456640 |
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| author | Giannou, Anastasios D. Ohm, Birte Zazara, Dimitra E. Lücke, Jöran Zhang, Tao Sabihi, Morsal Seeger, Philipp Oh, Jun Grotelüschen, Rainer Busch, Philipp Mann, Oliver Hackert, Thilo Izbicki, Jakob R. Yamada, Yoshito Huber, Samuel Jungraithmayr, Wolfgang |
| author_facet | Giannou, Anastasios D. Ohm, Birte Zazara, Dimitra E. Lücke, Jöran Zhang, Tao Sabihi, Morsal Seeger, Philipp Oh, Jun Grotelüschen, Rainer Busch, Philipp Mann, Oliver Hackert, Thilo Izbicki, Jakob R. Yamada, Yoshito Huber, Samuel Jungraithmayr, Wolfgang |
| author_sort | Giannou, Anastasios D. |
| collection | PubMed |
| description | The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).(1) |
| format | Online Article Text |
| id | pubmed-10679946 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-106799462023-11-10 Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies Giannou, Anastasios D. Ohm, Birte Zazara, Dimitra E. Lücke, Jöran Zhang, Tao Sabihi, Morsal Seeger, Philipp Oh, Jun Grotelüschen, Rainer Busch, Philipp Mann, Oliver Hackert, Thilo Izbicki, Jakob R. Yamada, Yoshito Huber, Samuel Jungraithmayr, Wolfgang STAR Protoc Protocol The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).(1) Elsevier 2023-11-10 /pmc/articles/PMC10679946/ /pubmed/37950863 http://dx.doi.org/10.1016/j.xpro.2023.102701 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Protocol Giannou, Anastasios D. Ohm, Birte Zazara, Dimitra E. Lücke, Jöran Zhang, Tao Sabihi, Morsal Seeger, Philipp Oh, Jun Grotelüschen, Rainer Busch, Philipp Mann, Oliver Hackert, Thilo Izbicki, Jakob R. Yamada, Yoshito Huber, Samuel Jungraithmayr, Wolfgang Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title | Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title_full | Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title_fullStr | Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title_full_unstemmed | Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title_short | Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| title_sort | protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679946/ https://www.ncbi.nlm.nih.gov/pubmed/37950863 http://dx.doi.org/10.1016/j.xpro.2023.102701 |
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